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Characterization of Rhodococcus wratislaviensis strain J3 that degrades 4-nitrocatechol and other nitroaromatic compounds

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Abstract

The bacterial strain J3 was isolated from soil by selective enrichment on mineral medium containing 4-nitrocatechol as the sole carbon and energy source. This strain was identified as Rhodococcus wratislaviensis on the basis of morphology, biochemical, physiological and chemotaxonomic characterization and complete sequencing of the 16S rDNA gene. The isolated bacterium could utilize 4-nitrocatechol, 3-nitrophenol and 5-nitroguaiacol as sole carbon and energy sources. Stoichiometric release of nitrites was measured during degradation of 4-nitrocatechol both in growing cultures and for stationary phase cells. The J3 strain was unable to degrade 4-nitroguaiacol, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-difluoro-6-nitrophenol. The J3 strain is deposited in the Czech Collection of Microorganisms as CCM 4930.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic

    Jitka Navrátilová, Ludmila Tvrzová & Miroslav Němec

  2. Centre of Microbiology, Immunology and Parasitology, Regional Institute of Public Health, Partyzánské náměstí 7, 702 00, Ostrava, Czech Republic

    Eva Durnová

  3. DSMZ – German Collection of Microorganisms and Cell Cultures, Mascheroder Weg 1b, 38124, Braunschweig, Germany

    Cathrin Spröer

  4. Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic

    Ivo Sedláček

  5. Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic

    Jiří Neča

Authors
  1. Jitka Navrátilová
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  2. Ludmila Tvrzová
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  3. Eva Durnová
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  4. Cathrin Spröer
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  5. Ivo Sedláček
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  6. Jiří Neča
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  7. Miroslav Němec
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Correspondence to Jitka Navrátilová.

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Navrátilová, J., Tvrzová, L., Durnová, E. et al. Characterization of Rhodococcus wratislaviensis strain J3 that degrades 4-nitrocatechol and other nitroaromatic compounds. Antonie Van Leeuwenhoek 87, 149–153 (2005). https://doi.org/10.1007/s10482-004-2480-z

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  • DOI: https://doi.org/10.1007/s10482-004-2480-z

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