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Genetic Diversity and Geographic Differentiation of the Giant Tiger Shrimp (Penaeus monodon) in Thailand Analyzed by Mitochondrial COI Sequences

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Abstract

Genetic diversity and geographic differentiation of the giant tiger shrimp, Penaeus monodon, in Thai waters (Satun, Trang, Phangnga, and Ranong in the Andaman Sea and Chumphon and Trat in the Gulf of Thailand) were examined by COI polymorphism (N = 128). We observed 28 COI mitotypes across all investigated individuals. The sequence divergence between pairs of mitotypes was 0.00–20.76%. A neighbor-joining tree clearly indicated lineage separation of Thai P. monodon and large nucleotide divergence between interlineage mitotypes but limited divergence between intralineage mitotypes. High genetic diversity was found (mean sequence divergence = 6.604%, haplotype diversity = 0.716–0.927, π = 2.936–8.532%). F-statistics (F ST) and an analysis of molecular variance (AMOVA) indicated that the gene pool of Thai P. monodon was not homogeneous but genetically differentiated intraspecifically (P < 0.05). Six samples of P. monodon could be allocated into three different genetic populations: Trat (A), Chumphon (B), and the Andaman samples Satun, Trang, Phangnga, and Ranong (C). Contradictory results regarding patterns of geographic differentiation previously reported by various molecular approaches were clarified by this study.

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Acknowledgment

This research was supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand, awarded to S.K. and B.K.

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Authors and Affiliations

  1. Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani, 12120, Thailand

    Bavornlak Khamnamtong & Sirawut Klinbunga

  2. Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Sirawut Klinbunga & Piamsak Menasveta

  3. Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Piamsak Menasveta

Authors
  1. Bavornlak Khamnamtong
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  2. Sirawut Klinbunga
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  3. Piamsak Menasveta
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Correspondence to Bavornlak Khamnamtong.

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Khamnamtong, B., Klinbunga, S. & Menasveta, P. Genetic Diversity and Geographic Differentiation of the Giant Tiger Shrimp (Penaeus monodon) in Thailand Analyzed by Mitochondrial COI Sequences. Biochem Genet 47, 42–55 (2009). https://doi.org/10.1007/s10528-008-9205-3

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  • DOI: https://doi.org/10.1007/s10528-008-9205-3

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