Abstract
Production of the artificial sweetener, lactosucrose, by various microorganisms containing levansucrase activity was investigated. Of the tested bacteria, Bacillus subtilis was the most effective producer using lactose as an acceptor and sucrose as a fructosyl donor. Lactosucrose production by this strain was optimal at pH 6.0 and 55 °C whereupon 181 g lactosucrose l−1 was produced from 225 g lactose l−1 and 225 g sucrose l−1 in 10 h.
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G Avigad (1957) ArticleTitleEnzymatic synthesis and characterization of a new trisaccharide, α-lactosy-β-fructofuranoside J. Biol. Chem. 229 121–129 Occurrence Handle1:CAS:528:DyaG1cXjsFKlsw%3D%3D Occurrence Handle13491565
HJ Choi CS Kim P Kim HC Jung DK Oh (2004) ArticleTitleLactosucrose bioconversion from lactose and sucrose by whole cells of Paenibacillus polymyxa harboring levansucrase activity Biotechnol. Prog. 20 1876–1879 Occurrence Handle10.1021/bp049770v Occurrence Handle1:CAS:528:DC%2BD2cXoslSju7g%3D Occurrence Handle15575726
K Fujita K Hara H Hashimoto S Kitahata (1990) ArticleTitleTransfructosylation catalyzed by β–fructofuranosidase I from Arthrobacter sp. K-1 Agric. Biol. Chem. 54 2655–2661 Occurrence Handle1:CAS:528:DyaK3MXjvVartw%3D%3D
K Fujita K Hara S Sakai T Miyake M Yamashita Y Tsunetomi T. Mutsuoka (1991a) ArticleTitleEffect of 4G-β-D-galactosylsucrose (lactosucrose) on intestinal flora and its digestibility in human Denpun Kagaku 38 249–255 Occurrence Handle1:CAS:528:DyaK38Xht1Onu78%3D
K Fujita T Osawa K Mikuni K Hara H Hashimoto S Kitahara (1991b) ArticleTitleProduction of lactosucrose by β-fructofuranosidase and its some physical properties Denpun Kagaku 38 1–7 Occurrence Handle1:CAS:528:DyaK3MXlvVWkt78%3D
M Kawase A Pilgrim T Araki K Hashimoto (2001) ArticleTitleLactosucrose production using a simulated moving bed reactor Chem. Eng. Sci. 56 453–458 Occurrence Handle10.1016/S0009-2509(00)00248-7 Occurrence Handle1:CAS:528:DC%2BD3MXht1yktL8%3D
T Ohkusa Y Ozaki C Sato K Mikuni H Ikeda (1995) ArticleTitleLong-term ingestion of lactosucrose increases Bifidobacterium sp. in human fecal flora Digestion 56 415–420 Occurrence Handle1:CAS:528:DyaK2MXptl2qtrg%3D Occurrence Handle8549886
A Pilgrim M Kawase M Ohashi K Fujita K Murakami K Hashimoto (2001) ArticleTitleReaction kinetics and modeling of the enzyme-catalyzed production of lactosucrose using β-fructofuranosidase from Arthrobacter sp. K-1 Biosci. Biotechnol. Biochem. 65 758–765 Occurrence Handle10.1271/bbb.65.758 Occurrence Handle1:CAS:528:DC%2BD3MXjsVags7k%3D Occurrence Handle11388450
A Takahama J Kuze S Okano K Akiyama T Nakane H Takahashi T Kobayashi (1991) ArticleTitleProduction of lactosucrose by Bacillus natto levansucrase and some properties of the enzyme Nippon Shokuhin Kogyo Gakkaishi 38 789–796 Occurrence Handle1:CAS:528:DyaK38XhslGrug%3D%3D
A Terada H Hara T Oishi S Matsui T Mitsuoka S Nakajyo I Fujimori K Hara (1992) ArticleTitleEffect of dietary lactosucrose on fecal flora and fecal metabolites of dogs Microb. Ecol. Health Dis. 5 87–92
A Terada H Hara S Kato T Kimura I Fujimori K Hara T Maruyama T Mitsuoka (1993) ArticleTitleEffect of lactosucrose (4G-β-D-galactosylsucrose) on fecal flora and fecal putrefactive products of cats J. Vet. Med. Sci. 55 291–295 Occurrence Handle1:CAS:528:DyaK3sXksVSrtbY%3D Occurrence Handle8513012
A Terada H Hara J Sakamoto N Sato S Takagi T Mitsuoka R Mino K Hara I Fujimori T Yamada (1994) ArticleTitleEffects of dietary supplementation with lactosucrose (4G-β-d-galactosylsucrose) on cecal flora, cecal metabolites, and performance in broiler chickens Polut. Sci. 73 1663–1672 Occurrence Handle1:CAS:528:DyaK2MXit1arsbY%3D
F Teramoto K Rokutan Y Kawakami Y Fujimura J Uchida K Oku M Oka M Yoneyama (1996) ArticleTitleEffect of 4G-β-d-galactosylsucrose (lactosucrose) on fecal microflora in patients with chronic inflammatory bowel disease J. Gastroenterol. 31 33–39 Occurrence Handle10.1007/BF01211184 Occurrence Handle1:CAS:528:DyaK28Xhtlynt7k%3D
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Park, NH., Choi &, HJ. & Oh, DK. Lactosucrose production by various microorganisms harboring levansucrase activity. Biotechnol Lett 27, 495–497 (2005). https://doi.org/10.1007/s10529-005-2539-6
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DOI: https://doi.org/10.1007/s10529-005-2539-6