Abstract
Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3′-hydroxyl group of quercetin and SOMT-2, which methylates at the 4′-hydroxyl group. Both OMT genes were driven by T7 promoters and had ribosome binding sites. Both ROMT-9 and SOMT-2 were successfully expressed in E. coli transformant harboring RSOMT. Reaction products of quercetin with E. coli transformant containing RSOMT showed two methylation products that corresponded to the 3′-methylated and the␣3′,4′-dimethylated quercetin, which were confirmed by NMR. More than 90% of quercetin was converted into the 3′,4′-dimethylated quercetin after 24 h incubation with E. coli transformant harboring RSOMT.
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Kim, B.G., Shin, K.H., Lee, Y. et al. Multiple Regiospecific Methylations of a Flavonoid by Plant O-Methyltransferases Expressed in E. coli. Biotechnol Lett 27, 1861–1864 (2005). https://doi.org/10.1007/s10529-005-3893-0
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DOI: https://doi.org/10.1007/s10529-005-3893-0