Abstract
Biphasic processes are used in whole-cell biotransformation to overcome the low water solubility of substrates and products as well as their inhibitory effects on the biocatalyst. Commercially available [NTf2]- and [PF6]-based ionic liquids (ILs) were used in a biphasic system for the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii. With the substrate at 5 g l−1 and a volume ratio of IL to buffer, buffer pH and cell density at, 1:9, 6.5, 16.8 gDW l−1, respectively, the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione was achieved with a yield of 70 % after 72 h using [BMIm][NTf2] in a 50 ml biphasic system. This is compared to a 30 % yield in a monophasic aqueous system. This suggests the potential industrial application of ILs-based biphasic systems for steroid biotransformation.
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Acknowledgments
This work was financially supported by Grants from the National High Technology Research and Development Program of China (863 Program) (No. 2011AA02A211), Applied Basic Research Programs of Science and Technology Commission Foundation of Tianjin (No.12JCQNJC06400) and the Foundation (No. 201004) of Tianjin Key Laboratory of Marine Resources and Chemistry (Tianjin University of Science & Technology).
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Mao, S., Hu, X., Hua, B. et al. 15α-Hydroxylation of a steroid (13-ethyl-gon-4-en-3,17-dione) by Penicillium raistrickii in an ionic liquid/aqueous biphasic system. Biotechnol Lett 34, 2113–2117 (2012). https://doi.org/10.1007/s10529-012-1016-2
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DOI: https://doi.org/10.1007/s10529-012-1016-2