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Rapid analysis of mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass by HPLC

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Abstract

HPLC using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyse mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass. PMP derivatives, including those of mannose, rhamnose, cellobiose, glucose, xylose and arabinose, were separated within 14 min with detection at 254 nm. The method was also suitable for xylo-oligosaccharides (XOS): PMP derivatives of xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose were well separated under the same conditions. The method was used to determine the mono-saccharide composition of Miscanthus and evaluate the production of XOS from enzymatic hydrolysis of crude xylan.

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References

  • Agblevor FA, Murden A, Hames BR (2004) Improved method of biomass sugars using high-performance liquid chromatography. Biotechnol Lett 26:1207–1210

    Article  PubMed  CAS  Google Scholar 

  • Akpinar O, Erdogan K, Bostanci S (2009) Enzymatic production of xylooligosaccharide from selected agricultural wastes. Food Bioprod Process 87:145–151

    Article  CAS  Google Scholar 

  • Brienzo M, Carvalho W, Milagres AM (2010) Xylooligosaccharides production from alkali-pretreated sugarcane bagasse using xylanases from Thermoascus aurantiacus. Appl Biochem Biotechnol 162:1195–1205

    Article  PubMed  CAS  Google Scholar 

  • Honda S, Akao E, Suzuki S, Okuda M, Kakehi K, Nakamura J (1989) High performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives. Anal Chem 180:351–357

    CAS  Google Scholar 

  • Krull LH, Inglett GE (1980) Analysis of neutral carbohydrates in agricultural residues by gas-liquid chromatography. J Agric Food Chem 28(5):917–919

    Article  PubMed  CAS  Google Scholar 

  • Lewandowski I, Clifton-Brown JC, Scurlock JMO, Huisman W (2000) Miscanthus: European experience with a novel energy crop. Biomass Bioenergy 19:209–227

    Article  CAS  Google Scholar 

  • Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69(6):627–642

    Article  PubMed  CAS  Google Scholar 

  • Lv Y, Yang XB, Zhao Y, Ruan Y, Yang Y, Wang ZZ (2009) Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection. Food Chem 112:742–746

    Article  CAS  Google Scholar 

  • Oliver RJ, Finch JW, Taylor G (2009) Second generation bioenergy crops and climate change: a review of the effects of elevated atmospheric Co2 and drought on water use and the implications for yield. GCB Bioenergy 1:97–114

    Article  CAS  Google Scholar 

  • Saha BC (2003) Hemicellulose bioconversion. J Ind Microbiol Biotechnol 30(5):279–291

    Article  PubMed  CAS  Google Scholar 

  • Sevcik RS, Mowery RA, Becker C, Chambliss CK (2011) Rapid analysis of carbohydrates in aqueous extracts and hydrolysates of biomass using a carbonate-modified anion-exchange column. J Chromatogr A 1218:1236–1243

    Article  PubMed  CAS  Google Scholar 

  • Slavin JL, Marlett JA (1983) Evaluation of high-performance liquid chromatography for measurement of the neutral saccharides in neutral detergent fiber. J Agric Food Chem 31(3):467–471

    Article  PubMed  CAS  Google Scholar 

  • Sluiter A, Hames B, Ruiz B, Scarlata C, Sluiter J, Templeton D (2006) Determination of sugars, byproducts and degradation products in liquid fraction process samples. NREL Technical Report NREL/TP-510-4263, p 11

  • Tamaki Y, Mazza G (2011) Rapid determination of carbohydrates, ash, and extractives contents of straw using attenuated total reflectance fourier transform mid-infrared spectroscopy. J Agric Food Chem 59(12):6346–6352

    Article  PubMed  CAS  Google Scholar 

  • Vázquez MJ, Alonso JL, Domínguez H, Parajó JC (2000) Xylooligosaccharides: manufacture and applications. Trends Food Sci Technol 11:387–393

    Article  Google Scholar 

  • Yang HY, Wang K, Song XL, Xu F (2011) Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa. Bioresource Technol 102:7171–7176

    Article  CAS  Google Scholar 

  • Zhang LY, Xu J, Zhang LH, Zhang WB, Zhang YK (2003) Determination of 1-phenyl-3-methyl-5-pyrazolone-labeled carbohydrates by liquid chromatography and micellar electrokinetic chromatography. J Chromatogr B 793:159–165

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31170067), the National Basic Research Program of China (973 Program) (Grant No: 2010CB732201) and the Fundamental Research Funds for the Central Universities (Grant No. 201112G026)

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Authors and Affiliations

  1. School of Energy Research, Xiamen University, Xiamen, 361005, People’s Republic of China

    Hailong Li, Chuannan Long, Jian Liu & Minnan Long

  2. School of Life Sciences, Xiamen University, Xiamen, 361005, People’s Republic of China

    Juan Zhou & Xiaobing Wu

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  1. Hailong Li
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  2. Chuannan Long
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  3. Juan Zhou
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  4. Jian Liu
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  5. Xiaobing Wu
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  6. Minnan Long
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Correspondence to Minnan Long.

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Li, H., Long, C., Zhou, J. et al. Rapid analysis of mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass by HPLC. Biotechnol Lett 35, 1405–1409 (2013). https://doi.org/10.1007/s10529-013-1224-4

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  • DOI: https://doi.org/10.1007/s10529-013-1224-4

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