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Fate and Transport of Organic Nitrogen in Minimally Disturbed Montane Streams of Colorado, USA

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Abstract

In two montane watersheds that receive minimal deposition of atmospheric nitrogen, 15–71% of dissolved organic nitrogen (DON) was bioavailable in stream water over a 2-year period. Discharge-weighted concentrations of bulk DON were between 102 and 135 μg/l, and the C:N ratio differed substantially between humic and non-humic fractions of DON. Approximately 70% of DON export occurred during snowmelt, and 40% of that DON was biologically available to microbes in stream sediments. Concentrations of bioavailable DON in stream water were 2–16 times greater than dissolved inorganic nitrogen (DIN) during the growing season, and bioavailable DON was depleted within 2–14 days during experimental incubations. Uptake of DON was influenced by the concentration of inorganic N in stream water, the concentration of non-humic DON in stream water, and the C:N ratio of the non-humic fraction of dissolved organic matter (DOM). Uptake of DON declined logarithmically as the concentration of inorganic N in stream water increased. Experimental additions of inorganic N also caused a decline in uptake of DON and net production of DON when the C:N ratio of non-humic DOM was high. This study indicates that the relative and absolute amount of bioavailable DON can vary greatly within and across years due to interactions between the availability of inorganic nutrients and composition of DOM. DOM has the potential to be used biotically at a high rate in nitrogen-poor streams, and it may be generated by heterotrophic microbes when DIN and labile DOM with low relative nitrogen content become abundant.

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References

  • M.A. Baker H.M. Valett C.N. Dahm (2000) ArticleTitleOrganic carbon supply and metabolism in a shallow groundwater ecosystem Ecology 81 3133–3148

    Google Scholar 

  • J.S. Baron D.H. Campbell (1997) ArticleTitleNitrogen fluxes in a high-elevation Colorado Rocky Mountain Basin Hydrol. Process. 11 783–799 Occurrence Handle10.1002/(SICI)1099-1085(199706)11:7<783::AID-HYP519>3.3.CO;2-L

    Article  Google Scholar 

  • T. Berman (2001) ArticleTitleThe role of DON and the effect of N:P ratios on occurence of cyanobacterial blooms: Implications from the outgrowth of Aphanizomenon in Lake Kinneret. Limnology and Oceanography 46 443–447

    Google Scholar 

  • E.S. Bernhardt G.E. Likens (2002) ArticleTitleDissolved organic carbon enrichment alters nitrogen dynamics in a forest stream Ecology 83 1689–1700

    Google Scholar 

  • J.N. Boyer P.M. Groffman (1996) ArticleTitleBioavailability of water extractable organic carbon fractions in forest and agricultural soil profiles Soil Biol. Biochem. 28 783–790 Occurrence Handle10.1016/0038-0717(96)00015-6

    Article  Google Scholar 

  • P.D. Brooks D.M. McKnight K.E. Bencala (1999) ArticleTitleThe relationship between soil heterotrophic activity, soil dissolved organic carbon (DOC) leachateand catchment-scale DOC export in headwater catchments Water Resour. Res. 35 1895–1902 Occurrence Handle10.1029/1998WR900125

    Article  Google Scholar 

  • D.A. Bronk P.M. Glibert T.C. Malone S. Banahan E Sahlsten (1998) ArticleTitleInorganic and organic nitrogen cycling in Chesapeake Bay: autotrophic versus heterotrophic processes and relationships to carbon flux Aquat. Microb. Ecol. 15 177–189

    Google Scholar 

  • D.A. Bronk B.B. Ward (1999) ArticleTitleGross and net nitrogen uptake and DON release in the euphotic zone of Monterey Bay, California Limnol. Oceanogr. 44 573–585

    Google Scholar 

  • D.A. Bronk B.B. Ward (2000) ArticleTitleMagnitude of dissolved organic nitrogen release relative to gross nitrogen uptake in marine systems Limnol. Oceanogr. 45 1879–1883

    Google Scholar 

  • I. Buffam J.N. Galloway L.K. Blum K.J. McGlathery (2001) ArticleTitleA stormflow/baseflow comparison of dissolved organic matter concentrations and bioavailability in an Appalachian stream Biogeochemistry 53 269–306 Occurrence Handle10.1023/A:1010643432253

    Article  Google Scholar 

  • K.L. Bushaw R.G. Zepp M.A. Tarr D. SchulzJander R.A. Bourbonniere R.E. Hodson W.L. Miller D.A. Bronk M.A. Moran (1996) ArticleTitlePhotochemical release of biologically available nitrogen from aquatic dissolved organic matter Nature 381 404–407 Occurrence Handle10.1038/381404a0

    Article  Google Scholar 

  • J.L. Campbell J.W. Hornbeck W.H. McDowell D.C. Buso J.B. Shanley G.E. Likens (2000) ArticleTitleDissolved organic nitrogen budgets for upland forested ecosystems in New England Biogeochemistry 49 123–142 Occurrence Handle10.1023/A:1006383731753

    Article  Google Scholar 

  • N.F. Caraco J.J. Cole (2003) The importance of organic nitrogen production in aquatic systems: a landscape perspective S.E.G. Findlay R.L. Sinsabaugh (Eds) Aquatic Ecosystems: Interactivity of Dissolved Organic Matter Elsevier Science USA 263–279

    Google Scholar 

  • N.F. Caraco G. Lampman J.J. Cole K.E. Limburg M.L. Pace D Fischer (1998) ArticleTitleMicrobial assimilation of DIN in a nitrogen rich estuary: implications for food quality and isotope studies Marine Ecol. Prog. Ser. 167 59–71

    Google Scholar 

  • W.S. Currie J.D. Aber W.H. McDowell R.D. Boone A.H. Magill (1996) ArticleTitleVertical transport of dissolved organic C and N under long-term N amendments in pine and hardwood forests Biogeochemistry 35 471–505 Occurrence Handle10.1007/BF02183037

    Article  Google Scholar 

  • M.L. Davi S. Bignami C. Milan M. Liboni M.G. Malfatto (1993) ArticleTitleDetermination of nitrate in surface waters by ion-exchange chromatography after oxidation of total organic nitrogen to nitrate J. Chromatogr. 644 345–348 Occurrence Handle10.1016/0021-9673(93)80718-N

    Article  Google Scholar 

  • W.K. Dodds (2003) ArticleTitleMisuse of inorganic N and soluble reactive P concentrations to indicate nutrient status of surface waters J. Nor. Am. Benthol. Soc. 22 171–181

    Google Scholar 

  • S Findlay (2003) Bacterial response to variation in dissolved organic matter S.E.G. Findlay R.L. Sinsabaugh (Eds) Aquatic Ecosystems: Interactivity of Dissolved Organic Matter Elsevier Science USA 363–377

    Google Scholar 

  • S. Findlay J.M. Quinn C.W. Hickey G. Burrell M Downes (2001) ArticleTitleEffects of land use and riparian flowpath on delivery of dissolved organic carbon to streams Limnol. Oceanogr. 46 345–355

    Google Scholar 

  • C.L. Goodale J.D. Aber W.H. McDowell (2000) ArticleTitleThe long-term effects of disturbance on organic and inorganic nitrogen export in the White Mountains, New Hampshire Ecosystems 3 433–450 Occurrence Handle10.1007/s100210000039

    Article  Google Scholar 

  • K. Grashoff (1976) Methods of Seawater Analysis Verlag Chmimie Weinheim 126–137

    Google Scholar 

  • P.M. Groffman D.R. Zak S. Christensen A. Mosier J.M. Tiedje (1993) ArticleTitleEarly spring nitrogen dynamics in a temperate forest landscape Ecology 74 1579–1585

    Google Scholar 

  • G. Guggenberger W. Zech H.R. Schulten (1994) ArticleTitleFormation and mobilization pathways of dissolved organic matter: evidence from chemical structural studies of organic matter fractions in acid forest floor solutions Org. Geochem. 21 51–66 Occurrence Handle10.1016/0146-6380(94)90087-6

    Article  Google Scholar 

  • L.B. Guldberg K. Finster N.O.G. Jorgenson M. Middelboe B.A. Lomstein (2002) ArticleTitleUtilization of marine sedimentary dissolved organic nitrogen by native anaerobic bacteria Limnol. Oceanogr. 47 1712–1722

    Google Scholar 

  • L.O. Hedin J.J. Armesto A.H. Johnson (1995) ArticleTitlePatterns of nutrient loss from unpollutedold-growth temperate forests: evaluation of biogeochemical theory Ecology 76 493–509

    Google Scholar 

  • E. Hood D.M. McKnight M.W. Williams (2003) ArticleTitleSources and chemical character of dissolved organic carbon across an alpine/subalpine ecotoneGreen Lakes Valley, Colorado Front RangeUnited States Water Resour. Res. 39 1188 Occurrence Handle10.1029/2002WR001738

    Article  Google Scholar 

  • K. Kaiser W. Zech (2000) ArticleTitleSorption of dissolved organic nitrogen by acid subsoil horizons and individual mineral phases Eur. J. Soil Sci. 51 403–411 Occurrence Handle10.1046/j.1365-2389.2000.00320.x

    Article  Google Scholar 

  • S. Kaushal M.W. Binford (1999) ArticleTitleRelationship between C:N ratios of lake sediments, organic matter sources, and historical deforestation in Lake PleasantMassachusetts J. Paleolimnol. 22 439–442 Occurrence Handle10.1023/A:1008027028029

    Article  Google Scholar 

  • S.S. Kaushal W.M. Lewis SuffixJr. (2003) ArticleTitlePatterns in the chemical fractionation of organic nitrogen in Rocky Mountain streams Ecosystems 6 483–492 Occurrence Handle10.1007/s10021-003-0175-3

    Article  Google Scholar 

  • C.L. Lagler P.F. Hendrix (1982) ArticleTitleEvaluation of persulfate digestion method for particulate nitrogen and phosphorus Water Res. 16 1451–1454 Occurrence Handle10.1016/0043-1354(82)90243-3

    Article  Google Scholar 

  • K. Lajtha B. Seely I Valiela (1995) ArticleTitleRetention and leaching losses of atmospherically-derived nitrogen in the aggrading coastal watershed of Waquoit Bay, MA Biogeochemistry 28 33–54 Occurrence Handle10.1007/BF02178060

    Article  Google Scholar 

  • W.M. Lewis SuffixJr. (1986) ArticleTitleNitrogen and phosphorus runoff losses from a nutrient-poor tropical moist forest Ecology 67 1275–1282

    Google Scholar 

  • W.M. Lewis SuffixJr. (2002) ArticleTitleYield of nitrogen from minimally disturbed watersheds of the United States Biogeochemistry 57 375–385 Occurrence Handle10.1023/A:1015709128245

    Article  Google Scholar 

  • W.M. Lewis Geoderma SuffixJr. M.C. Grant J.F. Saunders SuffixIII (1984a) ArticleTitleChemical patterns of atmospheric deposition in the state of Colorado Water Resour. Res. 10 161–1704

    Google Scholar 

  • W.M. Lewis SuffixJr. J.M. Melack W.H. McDowell M. McClain J.E. Richey (1999) ArticleTitleNitrogen yields from undisturbed watersheds in the Americas Biogeochemistry 46 149–162 Occurrence Handle10.1007/BF01007577

    Article  Google Scholar 

  • W.M. Lewis SuffixJr. J.F. Saunders SuffixIII D.W. Crumpacker SuffixSr. C. Brendecke (1984b) Eutrophication and Land Use: Lake Dillon, Colorado Springer-Verlag New York

    Google Scholar 

  • D.A. Lipson S.K. Schmidt R.K. Monson (1999) ArticleTitleLinks between microbial population dynamics and nitrogen availability in an alpine ecosystem Ecology 80 1623–1631

    Google Scholar 

  • G.M. Lovett K.C. Weathers W.V. Sobczak (1998) ArticleTitleNitrogen saturation and retention in forested watersheds of the Catskill Mountains, NY Ecol. Appl. 10 73–84 Occurrence Handle10.1016/S0929-1393(98)00039-0

    Article  Google Scholar 

  • J.H. McCutchan SuffixJr. W.M. Lewis SuffixJr. (2002) ArticleTitleRelative importance of carbon sources for macroinvertebrates in a Rocky Mountain stream Limnol. Oceanogr. 47 742–752

    Google Scholar 

  • W.H. McDowell W.S. Currie J.D. Aber Y. Yano (1998) ArticleTitleEffects of chronic nitrogen amendments on production of dissolved organic carbon and nitrogen in forest soils Water Air Soil Pollut. 105 175–182 Occurrence Handle10.1023/A:1005032904590

    Article  Google Scholar 

  • McDowell W.H., Magill A., Aitkenhead-Peterson J.A., Aber J.D., Merriam J. and Kaushal S.S. (In Press) Effects of chronic nitrogen amendment on dissolved organic matter and inorganic nitrogen in soil solution. Forest Ecol. Manage.

  • D.M. McKnight R. Harnish R.L. Wershaw J.S. Baron S. Schiff (1997) ArticleTitleChemical characteristics of particulatecolloidal, and dissolved organic material in Loch ValeWatershedRocky Mountain National Park Biogeochemistry 36 99–124 Occurrence Handle10.1023/A:1005783812730

    Article  Google Scholar 

  • G.J. Michaelson C.L. Ping G.W. Kling J.E. Hobbie (1998) ArticleTitleThe character and bioactivity of dissolved organic matter at thaw and in the spring runoff waters of the arctic tundra north slopeAlaska J. Geophys. Res. 103 28939–28946 Occurrence Handle10.1029/98JD02650

    Article  Google Scholar 

  • M.A. Moran R.E. Hodson (1990) ArticleTitleBacterial production on humic and nonhumic components of dissolved organic carbon Limnol. Oceanogr. 35 1744–1756

    Google Scholar 

  • P.J. Mulholland J.L. Tank D.M. Sanzone W.M. Wollheim B.J. Peterson J.R. Webster J.L. Meyer (2000) ArticleTitleNitrogen cycling in a forest stream determined by a N-15 tracer addition Ecol. Monogr. 70 471–493

    Google Scholar 

  • J. Murphy J.P. Riley (1962) ArticleTitleA modified single solution method for the determination of phosphate in natural waters Anal. Chim. Acta 27 31–36 Occurrence Handle10.1016/S0003-2670(00)88444-5

    Article  Google Scholar 

  • J.C. Neff D.U. Hooper (2002) ArticleTitleVegetation and climate controls on potential CO2DOC and DON production in northern latitude soils Global Change Biol. 8 872–884 Occurrence Handle10.1046/j.1365-2486.2002.00517.x

    Article  Google Scholar 

  • J.C. Neff F.S. Chapin P.M. Vitousek (2003) ArticleTitleThe role of dissolved organic nitrogen in nutrient retention and plant mineral nutrition; reconciling observations with ecological theory Front. Ecol. Environ. Sci. 1 205–211

    Google Scholar 

  • B.L. Peierls H.W. Paerl (1997) ArticleTitleBioavailability of atmospheric organic nitrogen deposition to coastal phytoplankton Limnol. Oceanogr. 42 181–1823

    Google Scholar 

  • S.S. Perakis L.O. Hedin (2002) ArticleTitleNitrogen losses from unpolluted South American forests mainly via dissolved organic compounds Nature 415 416–419 Occurrence Handle10.1038/415416a Occurrence Handle11807551

    Article  PubMed  Google Scholar 

  • B.J. Peterson M. Bahr G.W. Kling (1997) ArticleTitleA tracer investigation of nitrogen cycling in a pristine tundra river Can. J. Fish. Aquat. Sci. 54 2361–2367 Occurrence Handle10.1139/cjfas-54-10-2361

    Article  Google Scholar 

  • R.G. Qualls B.L. Haines (1991) ArticleTitleGeochemistry of dissolved organic nutrients in water percolating through a forested ecosystem Soil Sci. Soc. Am. J. 55 1112–1123

    Google Scholar 

  • R.G. Qualls B.L. Haines (1992) ArticleTitleBiodegradability of dissolved organic-matter in forest throughfall, soil solution, and stream water Soil Sci. Soc. Am. J. 56 578–586

    Google Scholar 

  • R.G. Qualls B.L. Haines W.T. Swank S.W. Tyler (2002) ArticleTitleRetention of soluble organic nutrients by a forested ecosystem Biogeochemistry 61 135–171 Occurrence Handle10.1023/A:1020239112586

    Article  Google Scholar 

  • R.G. Qualls C.J. Richardson (2003) ArticleTitleFactors controlling concentration, exportand decomposition of dissolved organic nutrients in the Everglades of Florida Biogeochemistry 62 197–229 Occurrence Handle10.1023/A:1021150503664

    Article  Google Scholar 

  • H.M. Rueth J.S. Baron (2002) ArticleTitleDifferences in Englemann spruce forest biogeochemistry east and west of the Continental Divide in ColoradoUSA Ecosystems 5 45–57 Occurrence Handle10.1007/s10021-001-0054-8

    Article  Google Scholar 

  • S.P. Seitzinger R.W. Sanders (1997) ArticleTitleContribution of dissolved organic nitrogen from rivers to estuarine eutrophication Marine Ecol. Prog. Ser. 159 1–12

    Google Scholar 

  • S.P. Seitzinger R.W. Sanders (1999) ArticleTitleAtmospheric inputs of dissolved organic nitrogen stimulate estuarine bacteria and phytoplankton Limnol. Oceanogr. 44 721–730

    Google Scholar 

  • S.P. Seitzinger R.W. Sanders R Styles (2002) ArticleTitleBioavailability of DON from natural and anthropogenic sources to estuarine phytoplankton Limnol. Oceanogr. 47 353–366

    Google Scholar 

  • J.O. Sickman J.M. Melack J.L. Stoddard (2002) ArticleTitleRegional analysis of inorganic nitrogen yield and retention in high-elevation ecosystems of the Sierra Nevada and Rocky Mountains Biogeochemistry 57 341–374 Occurrence Handle10.1023/A:1016564816701

    Article  Google Scholar 

  • P. Sollins F.M. McCorison (1981) ArticleTitleNitrogen and carbon solution chemistry of an old growth coniferous forest watershed before and after cutting Water Resour. Res. 17 1409–1418

    Google Scholar 

  • R. Stepanauskas H. Edling L.J. Tranvik (1999) ArticleTitleDifferential dissolved organic nitrogen availability and bacterial aminopeptidase activity in limnic and marine waters Microb. Ecol. 38 264–272 Occurrence Handle10.1007/s002489900176 Occurrence Handle10541788

    Article  PubMed  Google Scholar 

  • R. Stepanauskas N.O.G. Jorgenson O.R. Eigaard A. Zvikas L.J. Tranvik L. Leonardson (2002) ArticleTitleSummer inputs of riverine nutrients to the Baltic Sea: bioavailability and eutrophication relevance Ecol. Monogr. 72 579–597

    Google Scholar 

  • R. Stepanauskas H. Laudon N.O.G. Jorgenson (2000) ArticleTitleHigh DON bioavailability in boreal streams during a spring flood Limnol. Oceanogr. 45 1298–1307

    Google Scholar 

  • E.M. Thurman R.L. Malcolm (1981) ArticleTitlePreparative isolation of aquatic humic substances Environ. Sci. Technol. 15 463–466 Occurrence Handle10.1021/es00086a012

    Article  Google Scholar 

  • A.V. Vahatalo K. Salonen U. Munster M. Jarvinen R.G. Wetzel (2003) ArticleTitlePhotochemical transformation of allocthonous organic matter provides bioavailable nutrients in a humic lake Arch. Hydrobiol. 156 287–314 Occurrence Handle10.1127/0003-9136/2003/0156-0287

    Article  Google Scholar 

  • J.C. Valderrama (1981) ArticleTitleThe simultaneous analysis of total nitrogen and phosphorus in natural waters Marine Chem. 10 109–122 Occurrence Handle10.1016/0304-4203(81)90027-X

    Article  Google Scholar 

  • K.L. Vanderbilt K. Lajtha F.J. Swanson (2003) ArticleTitleBiogeochemistry of unpolluted forested watersheds in the Oregon Cascades: temporal patterns of precipitation and stream nitrogen fluxes Biogeochemistry 62 87–117 Occurrence Handle10.1023/A:1021171016945

    Article  Google Scholar 

  • P.M. Vitousek L.O. Hedin P.A. Matson J.H. Fownes J.C. Neff (1998) Within-system element cycles, input-output budgets and nutrient limitation P.M. Groffman M.L. Pace (Eds) Successes, Limitation and Frontiers in Ecosystem Science Springer New York 432–451

    Google Scholar 

  • T.N. Wiegner S.P. Seitzinger (2001) ArticleTitlePhotochemical and microbial degradation of external dissolved organic matter inputs to rivers Aquat. Microb. Ecol. 24 27–40

    Google Scholar 

  • J. Wikner R. Cuadros M. Jansson (1999) ArticleTitleDifferences in consumption of allocthonous DOC under limnic and estuarine conditions in a watershed Aquat. Microb. Ecol. 17 289–299

    Google Scholar 

  • M.W. Williams J.S. Baron N. Caine R. Sommerfeld R. Sanford (1996) ArticleTitleNitrogen saturation in the Rocky Mountains Environ. Sci. Technol. 30 640–646 Occurrence Handle10.1021/es950383e

    Article  Google Scholar 

  • A.P. Wolfe S.S. Kaushal J.R. Fulton D.M. McKnight (2002) ArticleTitleSpectrofluorescence of sediment humic substances and historical changes of lacustrine organic matter provenance in response to atmospheric nutrient enrichment Environ. Sci. Technol. 36 3217–3223 Occurrence Handle10.1021/es011215r Occurrence Handle12188343

    Article  PubMed  Google Scholar 

  • G.P. Zogg D.R. Zak K.S. Pregitzer A.J. Burton (2000) ArticleTitleMicrobial immobilization and the retention of anthropogenic nitrate in a northern hardwood forest Ecology 81 1856–1866

    Google Scholar 

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  1. Center for Limnology, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO, 80309-0216, USA

    Sujay S. Kaushal & William M. Lewis Jr.

  2. Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, 80309-0334, USA

    Sujay S. Kaushal

  3. Institute of Ecosystem Studies, Box AB (Route 44A), Millbrook, NY, 12545-0129, USA

    Sujay S. Kaushal

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Kaushal, S.S., Lewis, W.M. Fate and Transport of Organic Nitrogen in Minimally Disturbed Montane Streams of Colorado, USA. Biogeochemistry 74, 303–321 (2005). https://doi.org/10.1007/s10533-004-4723-5

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