biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:181-186, 2005 | DOI: 10.1007/s10535-005-1186-z

The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum

D. Kopecký1, J. Vagera1,2,*
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
2 Department of Cell Biology and Genetics, Faculty of Sciences, Palacky University, CZ-78371, Czech Republic

Pollen embryogenesis was successfully induced in Solanum nigrum L. (2n=6×=72). Stimulation of androgenesis expressed as the frequency of androgenic responsive anthers was observed after 10 and 20 mM ethyl methanesulphonate (EMS), 10 and 20 mM sodium azide (NaN3) and 0.2 mM N-nitroso-N-methylurea (MNU) treatment applied on seeds for 24 h. The frequency of androgenesis on the medium with sucrose was higher than on the medium with maltose. Androgenic regenerants originated also in the anthers collected from donor plants where survival after mutagenic treatment was lower than 50 %. Green haploid (3x), aneuploid (to 8x) and dihaploid (6x) plants were obtained. The high frequency of aneuploids among androgenic plants is explained by cell division irregularities in microsporial calli.

Keywords: enhanced androgenesis; mutagenesis; induced haploidy; anther culture; black nightshade
Subjects: androgenesis, anther culture, androgenic embryos; auxins; black nightshade, enhanced androgenesis; cDNA cloning; cytokinins; haploidy, induced; immunoblotting; intercellular spaces; Lupinus albus; mutagenesis; nutrient medium MS (Murashige and Skoog); pollen embryogenesis; polymerase chain reaction (PCR); protein, expression, differential, PR-10; Solanum nigrum; vascular tissues; white lupin, protein expression, PR-10

Received: May 10, 2004; Accepted: October 27, 2004; Published: June 1, 2005  Show citation

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Kopecký, D., & Vagera, J. (2005). The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum. Biologia plantarum, 49(2), 181-186. doi: 10.1007/s10535-005-1186-z
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