biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:187-191, 2012 | DOI: 10.1007/s10535-012-0040-3

Nitric oxide is involved in the regulation of ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress

C. Shan1,2, F. He3, G. Xu2, R. Han1,4, Z. Liang1,*
1 College of Life Science, Northwest A & F University, Yangling, P.R. China
2 Henan Institute of Science and Technology, Xinxiang, P.R. China
3 China Institute of Water Resources and Hydropower Research, Beijing, P.R. China
4 Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, P.R. China

This study investigated the regulation of ascorbate and glutathione metabolism by nitric oxide in Agropyron cristatum leaves under water stress. The activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), L-galactono-1,4-lactone dehydrogenase (GalLDH) and γ-glutamylcysteine synthetase (γ-ECS), and the contents of NO, reduced ascorbic acid (AsA), reduced glutathione (GSH), total ascorbate and total glutathione increased under water stress. These increases were suppressed by pretreatments with NO synthesis inhibitors N G-nitro-L-arginine methyl ester (L-NAME) and 4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). However, application of L-NAME and cPTIO to plants sufficiently supplied with water did not affect the activities of above mentioned enzymes and the contents of NO and above mentioned antioxidants. Pretreatments with L-NAME and cPTIO increased the malondialdehyde (MDA) content and electrolyte leakage of plants under water stress. Our results suggested that water stress-induced NO is a signal that leads to the upregulation of ascorbate and glutathione metabolism and has important role for acquisition of water stress tolerance.

Keywords: ascorbate peroxidase; cPTIO; electrolyte leakage; glutathione reductase; L-NAME; malondialdehyde
Subjects: ascorbate peroxidase; cPTIO; electrolyte leakage; glutathione reductase; L-NAME; malondialdehyde; nitric oxide; ascorbate; glutathione; water stress

Received: December 31, 2010; Accepted: February 10, 2011; Published: March 1, 2012  Show citation

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Shan, C., He, F., Xu, G., Han, R., & Liang, Z. (2012). Nitric oxide is involved in the regulation of ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Biologia plantarum, 56(1), 187-191. doi: 10.1007/s10535-012-0040-3
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