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Biomimetic hydrogels for biosensor implant biocompatibility: electrochemical characterization using micro-disc electrode arrays (MDEAs)

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Abstract

Our interest is in the development of engineered microdevices for continuous remote monitoring of intramuscular lactate, glucose, pH and temperature during post-traumatic hemorrhaging. Two important design considerations in the development of such devices for in vivo diagnostics are discussed; the utility of micro-disc electrode arrays (MDEAs) for electrochemical biosensing and the application of biomimetic, bioactive poly(HEMA)-based hydrogel composites for implant biocompatibility. A poly(HEMA)-based hydrogel membrane containing polyethylene glycol (PEG) was UV cross-linked with tetraethyleneglycol diacrylate following application to MDEAs (50 μm discs) and to 250 μm diameter gold electrodes within 8-well culture ware. Cyclic voltammetry (CV) of the MDEAs revealed a reduction in the apparent diffusion coefficient of ferrocenemonocarboxylic acid (FcCO2H), from 6.68 × 10−5 to 6.74 × 10−6 cm2/s for the uncoated and 6 μm thick hydrogel coated devices, respectively. Single frequency (4 kHz) temporal impedance measurements of the hydrogels in the 8-well culture ware showed a reversible 5% change in the absolute impedance of the hydrogels when exposed to a pH change between 6.1 to 7.2 and a 20% drop between pH 6.1 and 8.8.

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Funding

This work was supported by the US Department of Defense (DoDPRMRP) grant PR023081/DAMD17-03-1-0172 and by the Consortium of the Clemson University Center for Bioelectronics, Biosensors and Biochips (C3B).

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Authors and Affiliations

  1. Center for Bioelectronics, Biosensors and Biochips (C3B), Clemson University Advanced Materials Center, 100 Technology Drive, Anderson, SC, 29625, USA

    Gusphyl Justin, Stephen Finley, Abdur Rub Abdur Rahman & Anthony Guiseppi-Elie

  2. Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC, 29634, USA

    Stephen Finley & Anthony Guiseppi-Elie

  3. Department of Bioengineering, Clemson University, Clemson, SC, 29634, USA

    Anthony Guiseppi-Elie

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  1. Gusphyl Justin
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  2. Stephen Finley
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  3. Abdur Rub Abdur Rahman
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  4. Anthony Guiseppi-Elie
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Correspondence to Anthony Guiseppi-Elie.

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Justin, G., Finley, S., Abdur Rahman, A.R. et al. Biomimetic hydrogels for biosensor implant biocompatibility: electrochemical characterization using micro-disc electrode arrays (MDEAs). Biomed Microdevices 11, 103–115 (2009). https://doi.org/10.1007/s10544-008-9214-3

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