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Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip

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Abstract

We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

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References

  • K.O. Andresen, M. Hansen, M. Matschuk, S.T. Jepsen, H.S. Sorensen, P. Utko, D. Selmeczi, T.S. Hansen, N.B. Larsen, N. Rozlosnik, R. Taboryski, J Micromech Microeng 20, 055010 (2010)

    Article  Google Scholar 

  • J. Banchereau, R.M. Steinman, Nature 392, 245 (1998)

    Article  Google Scholar 

  • J.N. Blattman, P.D. Greenberg, Science 305, 200 (2004)

    Article  Google Scholar 

  • T. Geng, Y. Zhan, H.Y. Wang, S.R. Witting, K.G. Cornetta, C. Lu, J. Control. Release (2010)

  • P. Guermonprez, J. Valladeau, L. Zitvogel, C. Thery, S. Amigorena, Annu Rev Immunol 20, 621 (2002)

    Article  Google Scholar 

  • E.G. Guignet, T. Meyer, Nat Meth 5, 393 (2008)

    Article  Google Scholar 

  • N.J. Hallab, S. Anderson, M. Caicedo, A. Brasher, K. Mikecz, J.J. Jacobs, J Biomed Mater Res Part A 74A, 124 (2005)

    Article  Google Scholar 

  • T.S. Hansen, D. Selmeczi, N.B. Larsen, J Micromech Microeng 20, 015020 (2010)

    Article  Google Scholar 

  • A. Hosoi, Y. Takeda, K. Sakuta, S. Ueha, M. Kurachi, K. Kimura, R. Maekawa, K. Kakimi, Biochem Biophys Res Commun 371, 242 (2008)

    Article  Google Scholar 

  • K.S. Huang, Y.C. Lin, K.C. Su, H.Y. Chen, Biomed Microdevices 9, 761 (2007a)

    Article  Google Scholar 

  • K. Huang, Y. Lin, C. Su, C. Fang, Lab Chip 7, 86 (2007b)

    Article  Google Scholar 

  • T. Jain, J. Muthuswamy, Lab Chip 7, 1004 (2007)

    Article  Google Scholar 

  • M.F. Kalady, M.W. Onaitis, K.M. Padilla, S. Emani, D.S. Tyler, S.K. Pruitt, J Surg Res 105, 17 (2002)

    Article  Google Scholar 

  • P. Kalinski, J. Urban, R. Narang, E. Berk, E. Wieckowski, R. Muthuswamy, Future Oncol 5, 379 (2009)

    Article  Google Scholar 

  • M. Khine, A. Lau, C. Ionescu-Zanetti, J. Seo, L.P. Lee, Lab Chip 5, 38 (2005)

    Article  Google Scholar 

  • M. Khine, C. Ionescu-Zanetti, A. Blatz, L. Wang, L.P. Lee, Lab Chip 7, 457 (2007)

    Article  Google Scholar 

  • A. Lanzavecchia, F. Sallusto, Cell 106, 263 (2001)

    Article  Google Scholar 

  • W.G. Lee, U. Demirci, A. Khademhosseini, Integr Biol (Camb) 1, 242 (2009)

    Article  Google Scholar 

  • Y.C. Lin, M. Li, C.C. Wu, Lab Chip 4, 104 (2004)

    Article  Google Scholar 

  • S.N. Markovic, A.B. Dietz, C.W. Greiner, M.L. Maas, G.W. Butler, D.J. Padley, P.A. Bulur, J.B. Allred, E.T. Creagan, J.N. Ingle, D.A. Gastineau, S. Vuk-Pavlovic, J Transl Med 4, 35 (2006)

    Article  Google Scholar 

  • O. Met, J. Eriksen, I.M. Svane, Mol Biotechnol 40, 151 (2008)

    Article  Google Scholar 

  • O. Met, E. Balslev, H. Flyger, I.M. Svane, Breast Cancer Res. Treat. (2010)

  • P. Ponsaerts, V.F. Van Tendeloo, N. Cools, A. Van Driessche, F. Lardon, G. Nijs, M. Lenjou, G. Mertens, C. Van Broeckhoven, D.R. Van Bockstaele, Z.N. Berneman, Leukemia 16, 1324 (2002)

    Article  Google Scholar 

  • D.A. Puleo, W.W. Huh, J Appl Biomater 6, 109 (1995)

    Article  Google Scholar 

  • D. Ridgway, Cancer Invest 21, 873 (2003)

    Article  Google Scholar 

  • S. Saeboe-Larssen, E. Fossberg, G. Gaudernack, J Immunol Methods 259, 191 (2002)

    Article  Google Scholar 

  • N. Schaft, J. Dorrie, P. Thumann, V.E. Beck, I. Muller, E.S. Schultz, E. Kampgen, D. Dieckmann, G. Schuler, J Immunol 174, 3087 (2005)

    Google Scholar 

  • D.H. Schuurhuis, P. Verdijk, G. Schreibelt, E.H. Aarntzen, N. Scharenborg, A. de Boer, M.W. van de Rakt, M. Kerkhoff, M.J. Gerritsen, F. Eijckeler, J.J. Bonenkamp, W. Blokx, J.H. van Krieken, O.C. Boerman, W.J. Oyen, C.J. Punt, C.G. Figdor, G.J. Adema, I.J. de Vries, Cancer Res 69, 2927 (2009)

    Article  Google Scholar 

  • E.L. Smits, S. Anguille, N. Cools, Z.N. Berneman, V.F. Van Tendeloo, Hum Gene Ther 20, 1106 (2009)

    Article  Google Scholar 

  • P.J. Tacken, I.J. de Vries, R. Torensma, C.G. Figdor, Nat Rev Immunol 7, 790 (2007)

    Article  Google Scholar 

  • S. Tuyaerts, A. Michiels, J. Corthals, A. Bonehill, C. Heirman, C. de Greef, S.M. Noppe, K. Thielemans, Cancer Gene Ther 10, 696 (2003)

    Article  Google Scholar 

  • A. Valero, J.N. Post, J.W. van Nieuwkasteele, P.M. ter Braak, W. Kruijer, A. van den Berg, Lab Chip 8, 62 (2008)

    Article  Google Scholar 

  • V.F. Van Tendeloo, P. Ponsaerts, Z.N. Berneman, Curr Opin Mol Ther 9, 423 (2007)

    Google Scholar 

  • S.A. Yakovenko, Electroporation Chamber. 10/872242 (2005)

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Authors and Affiliations

  1. Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Ørsteds Plads bldg. 345ø, 2800, Kgs. Lyngby, Denmark

    David Selmeczi, Thomas S. Hansen & Niels B. Larsen

  2. Center for Cancer Immune Therapy (CCIT), Department of Hematology; and Department of Oncology, Herlev University Hospital, Herlev Ringvej 75, 2730, Herlev, Denmark

    Özcan Met & Inge Marie Svane

Authors
  1. David Selmeczi
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  2. Thomas S. Hansen
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  3. Özcan Met
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  4. Inge Marie Svane
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  5. Niels B. Larsen
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Correspondence to Niels B. Larsen.

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Selmeczi, D., Hansen, T.S., Met, Ö. et al. Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. Biomed Microdevices 13, 383–392 (2011). https://doi.org/10.1007/s10544-010-9507-1

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  • DOI: https://doi.org/10.1007/s10544-010-9507-1

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