Skip to main content
Erschienen in: Cellulose 5/2012

01.10.2012 | Original Paper

Synthesis and characterization of carboxymethyl cellulose-silver nanoparticle (AgNp)-silica hybrid for amylase immobilization

verfasst von: Vandana Singh, Shakeel Ahmad

Erschienen in: Cellulose | Ausgabe 5/2012

Einloggen

Aktivieren Sie unsere intelligente Suche um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

Carboxymethyl cellulose-silver nanoparticle (AgNp)-silica hybrids have been synthesized in a modified Stöber process. The hybrid synthesis was optimized to obtain an efficient immobilization matrix for diastase alpha amylase, a multimeric enzyme of high technological significance. The synthesized hybrids were characterized using FTIR, XRD, SEM, TGA and BET studies. The enzyme immobilization was done by adsorption and using the immobilized enzyme, the hydrolysis of soluble starch has been optimized in comparison to free enzyme. The optimum usable pH for the immobilized enzyme ranged from pH 4 to 5, while pH 5 was optimum pH for the free enzyme activity. The kinetic parameters for the immobilized, (K M = 3.4610 mg ml−1; V max = 6.3540 mg ml−1 min−1) and free enzyme (K M = 4.1664 mg ml−1; V max = 4.291 mg ml−1 min−1) hydrolysis indicated that the immobilization at the nanohybrid has significantly improved the catalytic property of the enzyme. In the immobilized state, the enzyme remained usable for many repeated cycles like our previous material, gum acacia-gelatin-AgNp-silica. Storage experiments indicated that the immobilization has increased the stability of the enzyme and also that AgNps play a role in stabilizing the immobilized enzyme.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Literatur
Zurück zum Zitat Bakunina Yu, Nedashkovskaya OI, Zvyagintseva TN, Shchipunov YuA (2006) Immobilization of α-galactosidase inside hybrid silica nanocomposites containing polysaccharides. Russ J Appl Chem 79:827CrossRef Bakunina Yu, Nedashkovskaya OI, Zvyagintseva TN, Shchipunov YuA (2006) Immobilization of α-galactosidase inside hybrid silica nanocomposites containing polysaccharides. Russ J Appl Chem 79:827CrossRef
Zurück zum Zitat Benmouhoub N, Simmonet N, Agoudjila N, Coradin T (2008) Aqueous sol-gel routes to bio-composite capsules and gels. Green Chem 10:957CrossRef Benmouhoub N, Simmonet N, Agoudjila N, Coradin T (2008) Aqueous sol-gel routes to bio-composite capsules and gels. Green Chem 10:957CrossRef
Zurück zum Zitat Copello GJ, Mebert AM, Raineri M, Pesenti MP, Diaz LE (2011) Removal of dyes from water using chitosan hydrogel/SiO2 and chitin hydrogel/SiO2 hybrid materials obtained by sol-gel method. J Hazard Mater 186:932CrossRef Copello GJ, Mebert AM, Raineri M, Pesenti MP, Diaz LE (2011) Removal of dyes from water using chitosan hydrogel/SiO2 and chitin hydrogel/SiO2 hybrid materials obtained by sol-gel method. J Hazard Mater 186:932CrossRef
Zurück zum Zitat Djabali D, Belhaneche N, Nadjemi B, Dulong V, Picton L (2009) Relationship between potato starch isolation methods and kinetic parameters of hydrolysis by free and immobilised α-amylase on alginate (from Laminaria digitata algae). J Food Compos Anal 22:563CrossRef Djabali D, Belhaneche N, Nadjemi B, Dulong V, Picton L (2009) Relationship between potato starch isolation methods and kinetic parameters of hydrolysis by free and immobilised α-amylase on alginate (from Laminaria digitata algae). J Food Compos Anal 22:563CrossRef
Zurück zum Zitat European Pharmacopeia, 5.0 (2005), Council of Europe (COE)—European Directorate for the Quality of Medicines (EDQM), Chapter 4, Reagents, section 4.1.3., Buffer solutions, p 430 European Pharmacopeia, 5.0 (2005), Council of Europe (COE)—European Directorate for the Quality of Medicines (EDQM), Chapter 4, Reagents, section 4.1.3., Buffer solutions, p 430
Zurück zum Zitat Hebeish AA, El-Rafie MH, Abdel-Mohdy FA, Abdel-Halim ES, Emam HE (2010) Carboxymethyl cellulose for green synthesis and stabilization of silver nanoparticls. Carbohydr Polym 82:933CrossRef Hebeish AA, El-Rafie MH, Abdel-Mohdy FA, Abdel-Halim ES, Emam HE (2010) Carboxymethyl cellulose for green synthesis and stabilization of silver nanoparticls. Carbohydr Polym 82:933CrossRef
Zurück zum Zitat Hu R, Lin L, Liu T, Ouyang P, He B, Liu S (2008) Reducing sugar content in hemicellulose hydrolysate by DNS method: a revisit. J Biobased Mater Bioener 2:156CrossRef Hu R, Lin L, Liu T, Ouyang P, He B, Liu S (2008) Reducing sugar content in hemicellulose hydrolysate by DNS method: a revisit. J Biobased Mater Bioener 2:156CrossRef
Zurück zum Zitat Lai S-M, Yang Ar J-M, Chen W-C, Hsiao J-F (2006) The properties and preparation of chitosan/silica hybrids using sol-gel process. Polym-Plast Technol Eng 45:997CrossRef Lai S-M, Yang Ar J-M, Chen W-C, Hsiao J-F (2006) The properties and preparation of chitosan/silica hybrids using sol-gel process. Polym-Plast Technol Eng 45:997CrossRef
Zurück zum Zitat Lee D, Cohen RE, Rubner MF (2005) Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. Langmuir 21:9651CrossRef Lee D, Cohen RE, Rubner MF (2005) Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. Langmuir 21:9651CrossRef
Zurück zum Zitat Manelius R, Bertoft E (1996) The effect of Ca2+-ions on the α-amylolysis of granular starches from oats and waxy-maize. J Cereal Sci 24:139CrossRef Manelius R, Bertoft E (1996) The effect of Ca2+-ions on the α-amylolysis of granular starches from oats and waxy-maize. J Cereal Sci 24:139CrossRef
Zurück zum Zitat Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426CrossRef Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426CrossRef
Zurück zum Zitat Mohan YM, Raju KM, Sambasivudu K, Singh S, Sreedhar B (2007) Preparation of acacia-stabilized silver nanoparticles: a green approach. J Appl Polym Sci 106:3375CrossRef Mohan YM, Raju KM, Sambasivudu K, Singh S, Sreedhar B (2007) Preparation of acacia-stabilized silver nanoparticles: a green approach. J Appl Polym Sci 106:3375CrossRef
Zurück zum Zitat Rao YN, Banerjee D, Datta A, Das SK, Guin R, Saha A (2010) Gamma irradiation route to synthesis of highly re-dispersible natural polymer capped silver nanoparticles. Radiat Phys Chem 79:1240CrossRef Rao YN, Banerjee D, Datta A, Das SK, Guin R, Saha A (2010) Gamma irradiation route to synthesis of highly re-dispersible natural polymer capped silver nanoparticles. Radiat Phys Chem 79:1240CrossRef
Zurück zum Zitat Rojas IA, Slunt JB, Grainger DW (2000) Polyurethane coatings release bioactive antibodies to reduce bacterial adhesion. J Control Rel 63:75CrossRef Rojas IA, Slunt JB, Grainger DW (2000) Polyurethane coatings release bioactive antibodies to reduce bacterial adhesion. J Control Rel 63:75CrossRef
Zurück zum Zitat Shchipunov YA, Karpenko TY (2004) Hybrid polysaccharide-silica nanocomposites prepared by the sol-gel technique. Langmuir 20:3882CrossRef Shchipunov YA, Karpenko TY (2004) Hybrid polysaccharide-silica nanocomposites prepared by the sol-gel technique. Langmuir 20:3882CrossRef
Zurück zum Zitat Shchipunov YA, Karpenko TY, Bakunina IY, Burtseva YV, Zvyagintseva TN (2004) A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides. J Biochem Biophys Meth 58:25CrossRef Shchipunov YA, Karpenko TY, Bakunina IY, Burtseva YV, Zvyagintseva TN (2004) A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides. J Biochem Biophys Meth 58:25CrossRef
Zurück zum Zitat Shchipunov YA, Karpenko TY, Krekoten AV (2005) Hybrid organic-inorganic nanocomposites fabricated with a novel biocompatible precursor using sol-gel processing. Compos Interface 11:587CrossRef Shchipunov YA, Karpenko TY, Krekoten AV (2005) Hybrid organic-inorganic nanocomposites fabricated with a novel biocompatible precursor using sol-gel processing. Compos Interface 11:587CrossRef
Zurück zum Zitat Singh V, Ahmed S (2012) Silver nanoparticle (AgNPs) doped gum acacia-gelatin-silica nanohybrid for diastase immobilization. Int J Biol Macromol 50:353CrossRef Singh V, Ahmed S (2012) Silver nanoparticle (AgNPs) doped gum acacia-gelatin-silica nanohybrid for diastase immobilization. Int J Biol Macromol 50:353CrossRef
Zurück zum Zitat Singh V, Kumar P (2011a) Carboxymethyl tamarind gum-silica nanohybrids for effective immobilization of amylase. J Mol Catal B Enzym 70:67CrossRef Singh V, Kumar P (2011a) Carboxymethyl tamarind gum-silica nanohybrids for effective immobilization of amylase. J Mol Catal B Enzym 70:67CrossRef
Zurück zum Zitat Singh V, Kumar P (2011b) Design of nanostructured tamarind seed kernel polysaccharide-silica hybrids for mercury (II) removal. Sep Purif Technol 46:825 Singh V, Kumar P (2011b) Design of nanostructured tamarind seed kernel polysaccharide-silica hybrids for mercury (II) removal. Sep Purif Technol 46:825
Zurück zum Zitat Singh V, Singh SK (2011) Synthesis and characterization of gum acacia inspired silica hybrid xerogels for mercury(II) adsorption. Inter J Biol macromol 48:445CrossRef Singh V, Singh SK (2011) Synthesis and characterization of gum acacia inspired silica hybrid xerogels for mercury(II) adsorption. Inter J Biol macromol 48:445CrossRef
Zurück zum Zitat Singh V, Singh SK, Pandey S, Sanghi R (2010) Adsorption behavior of potato starch-silica nanobiocomposite. Adv Mater Lett 1:40CrossRef Singh V, Singh SK, Pandey S, Sanghi R (2010) Adsorption behavior of potato starch-silica nanobiocomposite. Adv Mater Lett 1:40CrossRef
Zurück zum Zitat Siso MIG, Graber M, Condoret J-S, Combes D (1990) Effect of diffusional resistances on the action pattern of immobilized alpha-amylase. J Chem Technol Biotechnol 48:185CrossRef Siso MIG, Graber M, Condoret J-S, Combes D (1990) Effect of diffusional resistances on the action pattern of immobilized alpha-amylase. J Chem Technol Biotechnol 48:185CrossRef
Zurück zum Zitat Tischer W, Wedekind F (1999) Immobilized enzymes: methods and applications. Biocatalysis—From Discovery to Application. In: Fessner W-D (ed) Topics in Current Chemistry, Springer, Berlin Heidelberg, Vol. 200, pp 95–126 Tischer W, Wedekind F (1999) Immobilized enzymes: methods and applications. Biocatalysis—From Discovery to Application. In: Fessner W-D (ed) Topics in Current Chemistry, Springer, Berlin Heidelberg, Vol. 200, pp 95–126
Zurück zum Zitat Travan A, Pelillo C, Donati I, Marsich E, Benincasa M, Scarpa T, Semeraro S, Turco G, Gennaro R, Paoletti S (2009) Non-cytotoxic silver nanoparticle-polysaccharide nanocomposites with antimicrobial activity. Biomacromolecules 10:1429CrossRef Travan A, Pelillo C, Donati I, Marsich E, Benincasa M, Scarpa T, Semeraro S, Turco G, Gennaro R, Paoletti S (2009) Non-cytotoxic silver nanoparticle-polysaccharide nanocomposites with antimicrobial activity. Biomacromolecules 10:1429CrossRef
Zurück zum Zitat Wang G-H, Zhang L-M (2006) Using novel polysaccharide-silica hybrid material to construct an amperometric biosensor for hydrogen peroxide. J Phys Chem B 110:24864CrossRef Wang G-H, Zhang L-M (2006) Using novel polysaccharide-silica hybrid material to construct an amperometric biosensor for hydrogen peroxide. J Phys Chem B 110:24864CrossRef
Metadaten
Titel
Synthesis and characterization of carboxymethyl cellulose-silver nanoparticle (AgNp)-silica hybrid for amylase immobilization
verfasst von
Vandana Singh
Shakeel Ahmad
Publikationsdatum
01.10.2012
Verlag
Springer Netherlands
Erschienen in
Cellulose / Ausgabe 5/2012
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-012-9749-6

Weitere Artikel der Ausgabe 5/2012

Cellulose 5/2012 Zur Ausgabe