Abstract
Changes in agricultural production methods over the last century have caused a massive reduction and fragmentation of the area of European semi-natural grasslands. It remains unclear whether small and isolated grassland fragments can support viable plant populations in a sustainable way. In our study area in southern Belgium, the extent of calcareous grasslands was reduced from c. 650 ha in 1775 to less than 30 ha in 2004. We used AFLP markers to quantify the effects of present and historical grassland fragmentation on the genetic structure of 27 populations of the rare perennial plant species Globularia bisnagarica. Given the mixed breeding system of the species and the relatively small area of the studied system, the populations were characterized by high genetic differentiation (F st range: 0.42–0.48; Φst=0.53). A Mantel test revealed significant isolation by distance of the populations. Average within population genetic diversity, measured as expected heterozygosity or gene diversity, was low (H j =0.081) and was negatively related to population isolation. This suggests more gene flow into less isolated populations. Population size and local habitat characteristics did not significantly influence population genetic diversity. Both, high selfing rates in G. bisnagarica and a population genetic response to habitat fragmentation may explain our findings. Finally, a clear geographical clustering was observed, with cluster membership partially explainable by historical grassland connectivity. If populations indeed started to differentiate after fragmentation, this process was not (yet) strong enough to erase the genetic similarity between fragments that historically belonged to the same large grassland fragment.
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References
Bruun HH (2000) Patterns of species richness in dry grassland patches in an agricultural landscape. Ecography 23: 641–650
Bobbink R, Willems JH (1991) Impact of different cutting regimes on the performance of Brachypodium pinnatum in Dutch chalk grassland. Biol Conserv 56:1–21
Butaye J, Honnay O, Adriaens D, Delescaille LM, Hermy M (2005) Phytosociology and phytogeography of the calcareous grasslands on Devonian limestone in Southwest Belgium. Belgian J Bot 138:24–38
Couvet D (2002) Deleterious effects of restricted gene flow in fragmented populations. Conserv Biol 16:369–376
Decocq O, Delescaille LM, Dewitte T (2004) Une origine agropastorale. In: Colmant L (ed) Les Pelouses calcicoles en Région Wallonne. Entente Nationale pour la Protection de la Nature, Vierves, pp 3–16
Ellstrand N, Elam D (1993) Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol System 24:217–242
Fischer M, Stocklin J (1997) Local extinctions of plants in remnants of extensively used calcareous grasslands 1950–1985. Conserv Biol 11:727–737
Harrison S, Hastings A (1996) Genetic and evolutionary consequences of metapopulation structure. Trends Ecol Evol 11:180–183
Hensen I, Oberprieler C, Wesche K (2005) Genetic structure, population size, and seed production of Pulsatilla vulgaris Mill. (Ranunculaceae) in Central Germany. Flora 200:3–14
Honnay O, Bossuyt B (2005) Prolonged clonal growth: escape route or route to extinction?. Oikos 108:427–432
Honnay O, Coart E, Butaye J, Adriaens D, Roldan-Ruiz I (2006) Low effects of present and historical landscape configuration on population genetic structure of Anthyllis vulneraria. Biol Conserv 127:411–419
Höllander K, Jäger EJ (1998) Wuchsform und lebensgeschichte von Globularia Bisnagarica L. (G. punctata Lapeyr., Globulariaceae). Hercynia 31:143–171
Hooftman DAP, Billeter RC, Schmid B, Diemer M (2004) Genetic effects of habitat fragmentation on common species of Swiss fen meadows. Conserv Biol 19:1043–1051
Huff DR, Peakall R, Smouse PE (1993) RAPD variation within and among populations of outcrossing buffalograss (Buchloë dactyloides (Nutt.) Engelm). Theor Appl Genet 86:927–934
Klotz S, Kühn I, Durka W (2002) BIOLFLOR – Eine Datenbank zu biologisch-ökologischen Merkmalen der Gefäßpflanzen in Deutschland. – Schriftenreihe für Vegetationskunde 38, Bundesamt für Naturschutz. Bonn.
Keller LF, Waller DM (2002) Inbreeding effects in wild populations. Trends Ecol Evol 17:230–241
Kleijn D, Steinger T (2002) Contrasting effects of grazing and hay cutting on the spatial and genetic population structure of Veratrum album, an unpalatable, long-lived, clonal plant species. J Ecol 90:360–370
Krauss J, Klein A-M, Steffan-Dewenter I, Tscharntke T (2004) Effects of habitat area, isolation, and landscape diversity on plant species richness of calcareous grasslands. Biodivers Conserv 13:1427–1439
Lammi A, Siikamaki P, Mustajarvi K (1999) Genetic diversity, population size, and fitness in central and peripheral populations of a rare plant Lychnis viscaria. Conserv Biol 13:1069–1078
Leimu R, Mutikainen P (2005) Population history, mating system, and fitness variation in a perennial herb with a fragmented distribution. Conserv Biol 19:349–356
Lienert J, Fischer M, Schneller J, Diemer M (2002) Isozyme variability of the wetland specialist Swertia perennis (Gentianaceae) in relation to habitat size, isolation, and plant fitness. Am J Bot 89:801–811
Lindborg A, Eriksson O (2004) Historical landscape connectivity affects present plant species diversity. Ecology 85:1840–1845
Llorens TM, Ayre DJ, Whelan RJ (2004) Evidence for ancient genetic subdivision among recently fragmented populations of the endangered shrub grevillea caleyi (Protaceae). Heredity 92:519–526
Lynch M, Milligan BG (1994) Analysis of population genetic-structure with RAPD markers. Mol Ecol 3:91–99
Moilanen A, Nieminen M (2002) Simple connectivity measures in spatial ecology. Ecology 83:1131–1145
Nybom H, Bartish IV (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect Plant Ecol Evol Syst 3:93–114
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Pluess AR, Stocklin J (2004) Population genetic diversity of the clonal plant Geum reptans (Rosaceae) in the Swiss Alps. Am J Bot 91:2013–2021
Poschlod P, WallisDeVries MF (2002) The historical and socio-economic perspective of calcareous grasslands – lessons from the distant and recent past. Biol Conserv 104:361–376
Prentice HC, Lonn M, Rosquist G, Ihse M, Kindstrom. M (2006) Gene diversity in a fragmented population of Briza media: grassland continuity in a landscape context. J Ecol. 94:87–97
Prober SM, Brown AHD (1994) Conservation of the grassy white box woodlands: population genetics and fragmentation of Eucalyptus albens. Conserv Biol 8:1003–1013
Raijmann LL, Vanleeuwen NC, Kersten R, Oostermeijer JGB, Dennijs HCM, Menken SBJ (1994) Genetic-variation and outcrossing rate in relation to population size in Gentiana pneumonanthe. Conserv Biol 8:1014–1026
Richards CM (2000) Inbreeding depression and genetic rescue in a plant metapopulation. Am Nat 155:383–394
Roldán-Ruiz I, Dendauw J, van Bockstaele E, Depickere A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrass (Lolium spp.). Mol Breed 6:125–134
Schaal BA, Leverich WJ (1996) Molecular variation in isolated plant populations. Plant Species Biol 11:33–40
Schmidt K, Jensen K (2000) Genetic structure and AFLP variation of remnant populations in the rare plant Pedicularis palustris (Scrophulariaceae) and its relation to population size and reproductive components. Am J Bot 87:678–689
Sork VL, Nason J, Campbell DR, Fernandez JF (1999) Landscape approaches to historical and contemporary gene flow in plants. Trends Ecol Evol 14:219–224
Steffan-Dewenter I, Tscharntke T (1999) Effects of habitat isolation on pollinator communities and seed set. Oecologia 12:432–440
Tallmon DA, Luikart G, Waples RS (2004) The alluring simplicity and complex reality of genetic rescue. Trends Ecol Evol 19:489–496
van Treuren R., Bijlsma R, van Delden W, Ouborg NJ (1991) The significance of genetic erosion in the process of extinction. I. Genetic differentiation in salvia pratensis and Scabiosa columbaria in relation to population size. Heredity 66:181–189
van Rossum F, Campos de Sousa S, Triest L (2004) Genetic consequences of habitat fragmentation in an agricultural landscape on the common Primula veris and comparison with its rare congener, P. vulgaris. Conserv Genet 5:231–245
Vekemans X, Beauwens T, Lemaire M, Roldan-Ruiz I (2002) Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and a relationship between degree of homoplasy and fragment size. Mol Ecol 11:139–151
Volis S, Anikster Y, Olsvig-Whittaker L, Mendlinger S (2004) The influence of space in genetic–environmental relationships when environmental heterogeneity and seed dispersal occur at similar scale. Am Nat 163:312–327
Vos P, Hogers R, Bleeker M, Reijmans M, van de Lee R, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP, a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Young A, Boyle T, Brown T (1996) The population genetic consequences of habitat fragmentation for plants. Trends Ecol Evol 11:413–418
Young AG, Brown AHD, Zich FA (1999) Genetic structure of fragmented populations of the endangered Daisy Rutidosis leptorrhynchoides. Conserv Biol 13:256–265
Zhivotovski LA (1999) Estimating population structure in diploids with multilocus dominant DNA markers. Mol Ecol 8:907–913
Acknowledgments
This manuscript is part of the BIOCORE-research project which is supported by Federal Belgian Science Policy (formerly OSTC). Thanks to Tom Neels, Eric van Beek, and Jan Butaye for help with the fieldwork and to Sabine van Glabeke and Nancy Mergan for excellent assistance with laboratory work. HJ and DA have, respectively, a postdoctoral and doctoral fellowship from the Flemish Fund for Scientific Research (FWO). Thanks to L. Woué , L-M. Delescaille and forester ir. J-P. Scohy for their kind cooperation with BIOCORE.
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Honnay, O., Adriaens, D., Coart, E. et al. Genetic diversity within and between remnant populations of the endangered calcareous grassland plant Globularia bisnagarica L.. Conserv Genet 8, 293–303 (2007). https://doi.org/10.1007/s10592-006-9169-y
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DOI: https://doi.org/10.1007/s10592-006-9169-y