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Genetic structure of Saxifraga tridactylites (Saxifragaceae) from natural and man-made habitats

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Abstract

In contrast to many declining plant species Saxifraga tridactylites extended its distribution range in the man-made landscape of central Europe. The species naturally colonizes rocks and calcareous grasslands, but has also spread enormously in anthropogenic habitats such as railway constructions during the last decades. To analyze the genetic structure of the species 216 individuals from 8 populations in natural and 5 populations in man-made habitats were studied using AFLPs. The molecular analysis resulted in 250 scorable fragments. Population variability, measured as Nei’s gene diversity, Shannon’s Information Index and percentage of polymorphic bands, was slightly but not significantly higher in populations from natural habitats and was not correlated with population size. Mantel test indicated no significant correlation between pairwise genetic (ΦPT) and geographic distances. An analysis of molecular variance revealed significant differentiation between the two habitat types. About 9% variability were observed between natural and man-made habitats, 21% among populations within these two habitats and 70% within populations. In a neighbour joining cluster analysis populations from natural and man-made habitats were clearly separated from each other. Populations of S. tridactylites from man-made habitats do, therefore, not suffer from reduced genetic diversity. The observed genetic differentiation between populations from man-made and natural habitats could be due to reduced gene flow and/or habitat specific selection. However, the results of the study clearly demonstrate human impact on the genetic structure of plant populations in man-made landscapes.

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References

  • Amsellem L, Noyer JL, le Bourgeois T, Hossart-McKey M (2000) Comparison of genetic diversity of the invasive weed Rubus alceifolius Poir. (Rosaceae) in its native range and in areas of introduction, using amplified fragment length polymorphism (AFLP) markers. Mol Ecol 9:443–455

    Article  PubMed  CAS  Google Scholar 

  • Arnaud J-F, Madec L, Guiller A, Bellido A (2001) Spatial analysis of allozyme and microsatellite DNA polymorphisms in the land snail Helix aspersa (Gastropoda: Helicidae). Mol Ecol 10:1563–1576

    Article  PubMed  CAS  Google Scholar 

  • Austerlitz F, Jung-Muller B, Godelle B, Gouyon P-H (1997) Evolution of coalescence times, genetic diversity and structure during colonization. Theor Popul Biol 51:148–164

    Article  Google Scholar 

  • Bockelmann A-C, Reusch TBH, Bijlsma R, Bakker JP (2003) Habitat differentiation vs. isolation-by-distance: the genetic population structure of Elymus athericus in European salt marshes. Mol Ecol 12:505–515

    Article  PubMed  CAS  Google Scholar 

  • Bonnin I, Colas B, Bacles C, Holl AC, Hendoux F, Destiné B, Viards F (2002) Population structure of an endangered species living in contrasted habitats: Parnassia palustris (Saxifragaceae). Mol Ecol 11:979–990

    Article  PubMed  CAS  Google Scholar 

  • Brandes D (1983) Flora und Vegetation der Bahnhöfe Mitteleuropas. Phytocoenologia 11:31–115

    Google Scholar 

  • Briggs D, Walters SM (1997) Plant variation and evolution, 3 edn. Cambridge University Press, Cambridge

    Google Scholar 

  • Bruckmüller E, Hartmann P (2002) Putzger-Atlas und Chronik zur Weltgeschichte. Cornelsen, Berlin

    Google Scholar 

  • Düring C (2004) Flora und Vegetation der Bahn- und Hafenanlagen im Großraum Regensburg. Hoppea 65:71–293

    Google Scholar 

  • Eichhorn E (1958) Flora von Regensburg. Regensburg

  • Ellstrand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Ann Rev Ecol Syst 24:217–242

    Article  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Fischer M, Matthies D (1998) RAPD variation in relation to population size and plant fitness in the rare Gentianella germanica (Gentianaceae). Am J Bot 85:811–819

    Article  CAS  Google Scholar 

  • Friar EA, Boose DL, La Doux T, Roalson EH, Robichau RH (2001) Population structure in the endangered Mauna Loa silversword, Argyroxiphium kauense (Asteraceae), and its bearing on reintroduction. Mol Ecol 10:1657–1663

    Article  PubMed  CAS  Google Scholar 

  • Fürnrohr H (1839) Flora von Regensburg. Bauhof, Regensburg

    Google Scholar 

  • Galeuchet DJ, Perret C, Fischer M (2005) Microsatellite variation and structure of 28 populations of the common wetland plant, Lychnis flos-cuculi L., in a fragmented landscape. Mol Ecol 14:991–1000

    Article  PubMed  CAS  Google Scholar 

  • Gatterer K, Nezadal W, Fürnrohr F, Wagenknecht J, Welss W (2003) Flora des Regnitzgebietes. Die Farn- und Blütenpflanzen im zentralen Nordbayern. IHW-Verlag, Eching

    Google Scholar 

  • Gunter LE, Tuskan GA, Wullschleger SD (1996) Diversity of populations of switchgrass based on RAPD markers. Crop Sci 36:1017–1022

    Article  Google Scholar 

  • Hamrick JL, Godt MJW (1990) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding, and genetic resources. Sinauer, Sunderland, MA, pp 43–63

    Google Scholar 

  • Hsao JY, Lee M (1999) Genetic diversity and microgeographic differentiation of yushan cane (Yushania niitakayamensis; Poaceae) in Taiwan. Mol Ecol 8:263–270

    Article  Google Scholar 

  • Huff DR, Quinn JA, Higgins B, Palazzo AJ (1998) Random amplified polymorphic DNA (RAPD) variation among native little bluestem [Schizachyrium scoparium (Michx.) Nash] populations from sites of high and low fertility in forest and grassland biomes. Mol Ecol 7:1591–1597

    Article  CAS  Google Scholar 

  • Imbert E, Lefèvre F (2003) Dispersal and gene flow of Populus nigra (Salicaceae) along a dynamic river system. J Ecol 91:447–456

    Article  Google Scholar 

  • Ingvarsson PK (1997) The effect of delayed population growth on the genetic differentiation of local populations subject to frequent extinctions and recolonizations. Evolution 51:29–35

    Article  Google Scholar 

  • Kjølner S, Såstad M, Taberlet P, Brochmann C (2004) Amplified fragment length polymorphisms versus random amplified polymorphic DNA markers: clonal diversity in Saxifraga cernua. Mol Ecol 13:81–86

    Article  PubMed  CAS  Google Scholar 

  • Kölliker R, Stadelmann FJ, Reidy B, Nösberger J (1998) Fertilization and defoliation frequency affect genetic diversity of Festuca pratensis Huds. in permanent grasslands. Mol Ecol 7:1557–1567

    Article  Google Scholar 

  • Korneck D, Schnittler M, Vollmer I (1996) Rote Liste der Farn- und Blütenpflanzen (Pteridophyta et Spermatophyta) Deutschlands. Schrreihe Vegkd 28:21–188

    Google Scholar 

  • Krüger AM, Hellwig FH, Oberprieler C (2002) Genetic diversity in natural and anthropogenic inland populations of salt-tolerant plants: random amplified polymorphic DNA analyses of Aster tripolium L. (Compositae) and Salicornia ramosissima Woods (Chenopodiaceae). Mol Ecol 11:1647–1655

    Article  PubMed  Google Scholar 

  • Lenormand T (2002) Gene flow and the limits to natural selection. Trend Ecol Evol 17:183–189

    Article  Google Scholar 

  • Ludwig W (1996) Über die Ausbreitung von Saxifraga tridactylites L. entlang der Bahnlinien, zum Beispiel in Frankfurt a.M. und um Marburg. Hess Florist Briefe 45:1–6

    Google Scholar 

  • Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • Muluvi GM, Sprent JI, Soranzo N, Provan J, Odee D, Folkard G, McNicol JW, Powell W (1999) Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Mol Ecol 8:463–470

    Article  PubMed  CAS  Google Scholar 

  • Nei M, Maruyama T, Chakraborty R (1975) The bottleneck effect and genetic variability in populations. Evolution 29:1–9

    Article  Google Scholar 

  • Nevo E, Beiles A (1989) Genetic diversity of wild emmer wheat in Israel and Turkey. Structure, evolution and application in breeding. Theor Appl Genet 77:421–455

    Article  Google Scholar 

  • Nevo E, Beiles A, Krugman T (1988) Natural selection of allozyme polymorphisms: a microgeographic differentiation in wild emmer wheat (Triticum dicoccoides). Theor Appl Genet 75:529–538

    Article  Google Scholar 

  • Owuor ED, Fahima T, Beiles A, Korol A, Nevo E (1997) Population genetic response to microsite ecological stress in wild barley, Hordeum spontaneum. Mol Ecol 6:1177–1187

    Article  Google Scholar 

  • Parisod C, Trippi C, Galland N (2005) Genetic variability and founder effect in the pitcher plant Sarracenia purpurea (Sarraceniaceae) in populations introduced into Switzerland: from inbreeding to invasion. Ann Bot 95:277–286

    PubMed  CAS  Google Scholar 

  • Peakall R, Smouse PE (2001) GenalEx: Genetic Analysis in Excel. Australian National University. http://www.anu.edu.au/BoZo/GenAlEx, Canberra

  • Pluess AR, Stöcklin J (2004) Genetic diversity and fitness in Scabiosa columbaria in the Swiss Jura in relation to population size. Conserv Genet 5:145–156

    Article  CAS  Google Scholar 

  • Poschlod P, Bonn S (1998) Changing dispersal processes in the central European landscape since the last ice age: an explanation for the actual decrease of plant species richness in different habitats? Acta Bot Neerl 47:27–44

    Google Scholar 

  • Poschlod P, Kiefer S, Tränkle U, Fischer SF, Bonn S (1998) Plant species richness in calcareous grasslands as affected by dispersability in space and time. Appl Veg Sci 1:75–90

    Article  Google Scholar 

  • Reisch C, Anke A, Röhl M (2005) Molecular variation within and between ten populations of Primula farinosa (Primulaceae) along an altitudinal gradient in the northern Alps. Basic Appl Ecol 6:35–45

    Article  CAS  Google Scholar 

  • Reisch C, Poschlod P (2003) Intraspecific variation, land use and habitat quality – a phenologic and morphometric analysis of Sesleria albicans (Poaceae). Flora 198:321–328

    Google Scholar 

  • Reisch C, Poschlod P (2005) Land use affects gene flow between seasonal ecotypes of the grassland plant Scabiosa columbaria (Dipsacaeae). Ecology (submitted)

  • Reisch C, Poschlod P, Wingender R (2003a) Genetic differentiation among populations of Sesleria albicans Kit. ex Schultes (Poaceae) from ecologically different habitats in central Europe. Heredity 91:519–527

    Article  CAS  Google Scholar 

  • Reisch C, Poschlod P, Wingender R (2003b) Genetic variation of Saxifraga paniculata Mill. (Saxifragaceae): molecular evidence for glacial relict endemism in central Europe. Biol J Linn Soc 80:11–21

    Article  Google Scholar 

  • Rogers SO, Bendich AJ (1994) Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer Academic Press, Dordrecht, pp 1–8

    Google Scholar 

  • Sebald O, Seybold S, Philippi G, Wörz A (1998) Farn- und Blütenpflanzen Baden-Württembergs. Ulmer, Stuttgart

    Google Scholar 

  • Slatkin M (1977) Gene flow and genetic drift in a species subject to frequent local extinctions. Theor Popul Biol 12:253–262

    Article  PubMed  CAS  Google Scholar 

  • Slatkin M (1985) Gene flow in natural populations. Ann Rev Ecol Syst 16:393–430

    Article  Google Scholar 

  • Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236:787–792

    Article  PubMed  CAS  Google Scholar 

  • Tremetsberger K, Stuessy TF, Samuel RM, Baeza CM, Fay F (2003) Genetics of colonization in Hypochaeris tenuifolia (Asteraceae, Lactuceae) on Volcán Lonquimay, Chile. Mol Ecol 12:2649–2659

    Article  PubMed  CAS  Google Scholar 

  • Tutin TG, Heywood VH, Burgess NA, Moore DM, Valentine DH, Walters SM, Webb DA (1964) Flora Europaea. Cambridge University Press, Cambridge

    Google Scholar 

  • van Rossum F, Campos De Sousa S, Triest L (2004) Genetic consequences of habitat fragmentation in an agricultural landscape on the common Primula veris, and comparison with its rare congener, P. vulgaris. Conserv Genet 5:231–245

    Article  Google Scholar 

  • Vollmann F (1914) Flora von Bayern. Ulmer, Stuttgart

    Google Scholar 

  • Yeh FC, Yang RC, Boyles TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, Alberta

    Google Scholar 

  • Zawko G, Krauss SL, Dixon KW, Sivasithamparam K (2001) Conservation genetics of the rare and endangered Leucopogon obtectus (Ericaceae). Mol Ecol 10:2389–2396

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

The author thanks Peter Poschlod for his generous support, Petra Schitko for her assistance in the lab and Bernhard Hoiß for his help with the data analysis. Furthermore, I acknowledge Jörg Meister for his help with AFLP analysis and Sven Himmelreich for technical support. Christian Düring provided well-founded information about the distribution of S. tridactylites in the study area. Special thanks go to Markus Bernhard-Römermann, who provided very helpful comments on the manuscript.

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    Christoph Reisch

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Reisch, C. Genetic structure of Saxifraga tridactylites (Saxifragaceae) from natural and man-made habitats. Conserv Genet 8, 893–902 (2007). https://doi.org/10.1007/s10592-006-9244-4

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