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Mold spore penetration through wall service outlets: a pilot study

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Abstract

A pilot study was conducted to estimate fungal spore penetration for wall service outlets subjected to a constant air pressure. During the laboratory experiment, a wall chamber was fabricated, and telephone, electrical, and cable service outlets were installed. Penicillium chrysogenum spores were aerosolized into the chamber that was held under pressure. Spores that penetrated the outlets were funneled into an impinger for microscopic enumeration. Thirty trials were conducted for each of the five outlets (N = 150), and the wall chamber was decontaminated between trials. Results of an analysis of variance suggest wall service outlets allow spore penetration. The penetration factor for the telephone outlet was significantly greater than all other outlets (p < 0.05), and there was no difference in penetration between electrical outlets with and without plugs. Penetration factor differences were attributed to air leakage rates across the outlets. Due to the experimental design and equipment limitations, further research is needed to support these findings.

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References

  • Airaksinen, M., Kurnitski, J., Pasannen, P., & Seppanen, O. (2003). Fungal spore transport through a building structure. Indoor Air, 14, 92–104.

    Article  Google Scholar 

  • Aizenberg, V., Reponen, T., Grinshpun, S. A. & Willeke, K. (2000). Performance of air-o-cell, burkard, and button samplers for total enumeration of airborne spores. American Industrial Hygiene Association Journal, 61, 855–864.

    Article  CAS  Google Scholar 

  • Alvine, G. F., Rodgers, P., Fitzsimmons, K. M., & Ahrens, R. C. (1994). Disposable jet nebulizers: How reliable are they. Chest, 101, 316–319.

    Article  Google Scholar 

  • American Optical (1974). AO Spencer bright line hemacytometer. Buffalo, NY: American Optical Company.

    Google Scholar 

  • American Thoracic Society (1997). American thoracic society workshop, achieving healthy indoor air. American Journal of Respiratory and Critical Care Medicine, 3, 534–564.

    Google Scholar 

  • Buttner, M., Cruz-Perez, P., Stetzenbach, L. D. & Garrett, P. J. (1999). Dispersal of fungal spores from three types of air handling system duct material. Aerobiologia, 15, 1–8.

    Article  Google Scholar 

  • Buttner, M., Cruz-Perez, P., Stetzenbach, L. D., Garrett, P. J., & Luedtke, A. E. (2002). Measurement of airborne fungal spore dispersal from three types flooring materials. Aerobiologia, 18, 1–11.

    Article  Google Scholar 

  • Centers for Disease Control and Prevention (2006). Surveillance in hurricane evacuation centers—Louisiana, September–October 2005. Morbidity and Mortality Weekly Report, 2, 32–35.

    Google Scholar 

  • Chen, P., & Li, C. (2005). Sampling performance for bioaerosols by flow cytometry with fluorochrome. Aerosol Science and Technology, 39, 231–237.

    Article  CAS  Google Scholar 

  • Chung, W., Wi, S. & Park, H. B. B. (1999). Microscopic observation of wood-based composites exposed to fungal deterioration. Journal of Wood Science, 45, 64–68.

    Article  CAS  Google Scholar 

  • Finnegan, M. J., Pickering, C. A. & Burge, P. S. (1984). The sick building syndrome: Prevalence studies. British Medical Journal, 289, 1573–1575.

    Article  CAS  Google Scholar 

  • Fazio, P., Bartlett, K., Yang, D., Rao, J. & Miao, G. (2005). Development of experimental procedure to evaluate potential movement of mold spores from wall cavity to indoor environment. In Proceedings of the 10th International Conference on Building Science, Ottawa.

  • Grant, I. W. B. (1985). The sick building syndrome. British Medical Journal, 290, 321.

    Article  Google Scholar 

  • Gravesen, S., Nielsen, P. A. & Nielsen K. F. (1997) “Microfungi in water damaged buildings”, SBI Report Number 282, Danish Building Research Institute, Copenhagen: Denmark.

    Google Scholar 

  • Heinsohn, P. A. (2007). Conducting the building mold investigations. In C. S. Yang & P. Heinsohn (Eds.), Sampling and analysis of indoor microorganisms (pp. 21). Hoboken, NJ: Wiley.

    Google Scholar 

  • Hillenga, D. J., Siep Vander Molen, H. J. M. V., Driessen, J. M. & Konings, M. M. (1995). Penicillium chrysogenum takes up the Penicillin G precursor phenylacetic acid by passive diffusion. Applied Environmental Microbiology, 61, 2589–2595.

    CAS  Google Scholar 

  • Kildeso, J., Wertz, H., Nielsen, K. F., Kruse, P., Wilkins, K., Thrane, U., et al. (2003). Determination of fungal spore release from wet building materials. Indoor Air, 13, 148–153.

    Article  CAS  Google Scholar 

  • Laks, P. E., Richter, D. L. & Larkin, G. M. (2002). Fungal susceptibility of interior commercial building panels. Forest Products Journal, 52, 41–44.

    Google Scholar 

  • Lawton, M. D., Dales, R. E., & White, J. (1998). The influence of house characteristics in a Canadian community on microbiological contamination. Indoor Air, 8, 2–11.

    Article  CAS  Google Scholar 

  • Liu, D. & Nazaroff, W. W. (2002). Particle penetration through windows. In Proceedings Indoor Air. Retrieved 26 August 2007 from http://buildingairflow.lbl.gov/pubs/Penetrate Windows.pdf.

  • Liu, D. & Nazaroff, W. W. (2003). Particle penetration through building cracks. Aerosol Science and Technology, 37, 565–573.

    Article  CAS  Google Scholar 

  • Mailer, J. S. & Mason, B. (2001) Penicillin: Medicine’s wartime wonder drug and its production at Illinois. Retrieved 31 May 2008 from http://www.lib.niu.edu/ipo/2001/iht810139.html.

  • Mainelis, G., Gorny, R. L., Willeke, K. & Reponen, T. (2005). Rapid counting of liquid-borne micro organisms by light scattering spectrometry. Annals of Agricultural and Environmental Medicine, 12, 141–148.

    Google Scholar 

  • Mosley, R. B., Greenwell, D. J., Sparks, L. E., Guo, Z., Tucker, W. G., Fortmann, R., et al. (2001). Penetration of ambient fine particles into the indoor environment. Aerosol Science and Technology, 34, 127–136.

    Article  CAS  Google Scholar 

  • Murtoniemi, T., Hirvonen, M. R., Nevalainen, A. & Suutari, M. (2003). The relation between growth of four microbes on six different plasterboards and biological activity of spores. Indoor Air, 13, 65–73.

    Article  CAS  Google Scholar 

  • Pérez, H. (2004). Detection of indoor airborne fungal contamination through examination of building HVAC filters. Doctoral dissertation, Health Sciences, Occupational Health and Safety Purdue University, Lafayette.

  • Price, D. L. & Ahearn, D. G. (1999). Sanitation of wallboard colonized with Stachybotrys chartarum. Microbiology, 39, 21–26.

    CAS  Google Scholar 

  • Rao, J., Miao, G., Yang, D. Q., Bartlett, K. & Fazio, P. (2006). Experimental evaluation of potential movement of airborne mold spores out of building envelope cavities using full-size wall panels. In P. Fazio, J. Rao & G. Desmarais (Eds.), Research in building physics and building engineering (pp. 845–852). London: Taylor & Francis.

    Google Scholar 

  • Schmechel, D., Simpson, J. P. & Lewis, D. M. (2005). The production and characterization of monoclonal antibodies to the fungus Aspergillus versicolor. Indoor Air, 15, 11–19.

    Article  Google Scholar 

  • Stolwijk, A. J. (1991). Sick building syndrome. Environmental Health Perspectives, 95, 99–100.

    Article  CAS  Google Scholar 

  • United States Environmental Protection Agency (2001). Mold remediation in schools and commercial buildings. EPA 402-K-01-001.

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Correspondence to Brad Muise.

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Muise, B., Seo, DC., Blair, E.E. et al. Mold spore penetration through wall service outlets: a pilot study. Environ Monit Assess 163, 95–104 (2010). https://doi.org/10.1007/s10661-009-0819-7

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  • DOI: https://doi.org/10.1007/s10661-009-0819-7

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