Abstract
The genotoxicity of certain water bodies was evaluated employing the DNA repair defective mutants of Escherichia coli, induction of prophage lamda in the lysogen and the plasmid nicking assay. All the test DNA repair defective mutants invariably exhibited more sensitivity than their isogenic wild-type strains but distinctive patterns against the three water samples viz. industrial waste water and the groundwater samples obtained from industrial estate of Aligarh as well as river water of Yamuna at Agra. A significant level of phage induction was also recorded in the test system exhibiting maximum induction in case of industrial waste water followed by that in river and groundwater samples, respectively. The single- and double-strand breaks were also observed in the plasmid DNA treated with industrial waste water and the river water samples. These findings are suggestive of the DNA damage induced by the test samples with the probable role of SOS repair in E. coli.
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References
Alam, M. Z. , Ahmad, S., & Malik, A. (2009). Genotoxic and mutagenic potential of agricultural soil irrigated with tannery effluents at Jajmau (Kanpur), India. Archives of Environmental Contamination and Toxicology, 57, 463–476.
American Public Health Association (APHA) (1998). In L. S. Clesceri, A. E. Greenberg, & A. D. Eaton (Eds.), Standard methods for the examination of water and wastewater (20th ed.). Washington: APHA.
Azuma, A., Huang, P., Matsuda, A., & Plunkett, W. (2001). 2-C-cyano 2-deoxy-1-beta-D-arabino-pentafuranosyl-cytosine: a novel anticancer analog that causes both DNA strand breaks and G (2) arrest. Molecular Pharmocology, 59(4), 725–731.
Bagg, A., Kenyon, C. J. , & Walker, G. C. (1981). Inducibility of a gene product required for UV and chemical mutagenesis in Eschericia coli. PNAS, 78(9), 5749–5753.
Brazmanova, J., Dudas, A., & Henriques, J. A. (2001). Repair of oxidative DNA damage-an important factor reducing cancer risk. Neoplasma, 48(2), 85–93.
Cabrera, G. (2000). Effect of five dietary antimutagens on the genotoxicity of six mutagens in the microscreen prophage induction assay. Environmental and Molecular Mutagenesis, 36(3), 206–220.
Courcelle, J., Ganesan, A. K. , & Hanawalt, P. C. (2001). Therefore, what are recombination proteins there for. Bioessays, 23(5), 463–470.
Coureclle, J., & Hanawalt, P. C. (2001). Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination. PNAS, 98(15), 8196–8202.
d’Ari, R. (1985). The SOS system. Biochimie, 67(3–4), 343–347.
De Marini, D. M. , Perry, E., & Sheltor, M. L. (1994). Dichloroacetic acid and related compounds: induction of prophage in E. coli and mutagenicity and mutation spectra in Salmonella TA100. Mutagenesis, 9(5), 429–437.
Dutka, B. J. (1996). Bioassays-summary of the 18 years work at NWRI, Canada, Water-Tox Workshop I, Theme paper, IDRC, Canada.
Ewig, R. A. , & Kohn, K. W. (1977). DNA damage and repair in mouse leukemia L1210 cells treated with nitrogen mustard, 1,3-bis (2-chloroethyl)-1 nitrosourea and other nitrosoureas. Cancer Research, 37, 2114–2122.
Fatima, R. A. , & Ahmad, M. (2006). Genotoxicity of industrial wastewaters obtained from two different pollution sources in northern India: a comparison of three bioassays. Mutation Research, 609, 81–91.
Gottesman, S. (1981). Genetic control of SOS system in E.coli. Cell, 23(1), 1–2.
Hellmer, L., & Boldsfoldi, G. (1992). An evaluation of the E.coli K-12 uvrB/recA DNA repair host mediated assay. I- In vitro sensitivity of the bacteria to 61 compounds. Mutation Research, 272(2):145–160.
ISGE (1990). Integrated study of Ganga Ecosystem between Kachla to Kannauj, Ganga Project Report. New Delhi: Department of Environment and Forests, Govt. of India.
Kenyon, C. J. , & Walker, G. C. (1981). Expression of E.coli uvrA gene is inducible. Nature, 289(5800), 808–810.
Kerklaan, P. R. , Bouter, S., Van Elburg, P. A. , & Mohan, G. R. (1985). Evaluation of the DNA - repair host mediated assay. I - Induction of repairable DNA damage in E.coli cells recovered from liver, spleen, lungs, kidney and the blood stream of mice treated with methylating carcinogens. Mutation Research, 148 (1–2), 1–12.
Leadon, S. A. (1996). Repair of DNA damage produced by ionizing radiations: a minireview. Seminars in Radiation Oncology, 6(4), 295–305.
Leifer, Z., Kada, T., Mandel, M., Zeiger, E., Stafford, R., & Rosen Kranz, H. S. (1981). An evaluation of tests using DNA repair-deficient bacteria for predicting genotoxicity and carcinogenicity. A report to the US EPA’s Gene-Tox Program. Mutation Research, 87(3), 211–297.
Lutz, W. K. (1979). In vivo covalent binding of organic chemicals to DNA as a quantitative indicator in the process of chemical carcinogenesis. Mutation Research, 65(4), 289–356.
Malik, A., & Ahmad, M. (1995). Genotoxicity of some waste waters of India. Environmental Toxicology and Water Quality, 10, 287–293.
Maron, D., & Ames, B. N. (1983). Revised methods for the Salmonella mutagenicity test. Mutation Research, 113(3–4), 173–215.
Maru, G. B. , & Bhide, S. V. (1989). DNA damage as a marker of exposure to carcinogens. Journal of Toxicol-Toxin Reviews, 8(1 and 2), 177–194.
Modrich, P. (1991). Mechanisms and biological effects of mismatch repair. Annual Review of Genetics, 25, 229–253.
Muller, J., & Janz, S. (1992). Assessment of oxidative DNA damage in the oxyR-deficient SOS chromotest strain Eschericia coli PQ300. Environmental and Molecular Mutagenesis, 20(4), 297–306.
Pastink, A., Ecken, J. C. , & Lohman, P. H. (2001). Genomic integrity and the repair of double strand breaks. Mutation Research, 480–481, 37–50.
Peltomaki, P. (2001). DNA mismatch repair and cancer. Mutation Research, 488(1), 77–85.
Qadri, S. A. , & Ahmad, M. (1994). Damage and mutagenesis of E. coli and bacteriophage λ induced by oxathiolane and aziridinyl steroids. Mutation Research, 311(2), 199–208.
Quillardet, P., Huissman, O., d’Ari, R., & Hofnung, M. (1982). SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. PNAS, 79(19), 5971–5975.
Rahman, A., Shahbuddin, S. M. H. , & Parish, J. H. (1990). Complexes involving quercetin, DNA and Cu(II). Carcinogenosis, 11(11), 2001–2003.
Rehana, Z., Malik, A., & Ahmad, M. (1995). Mutagenic activity of the ganges water with special reference to the pesticide pollution in the river between Kachla to Kannauj (U.P.), India. Mutation Research, 343(2–3), 137–144.
Rehana, Z., Malik, A., & Ahmad, M. (1996). Genotoxicity of the Ganges water at Narora (U.P.), India. Mutation Research, 367(4), 187–193.
Schwarz, M., Hummel, J., Appel, K. E. , Rickart, R., & Kunz, W. (1979). DNA damage induced in vivo evaluated with a non radioactive elution technique. Cancer Letter, 6(4–5), 221–226.
Vargas, V. M. , Migliavacca, S. B. , de Melo, A. C. , Horn, R. C. , Guidobono, R. R. , de Sa Ferreira, I. C. , et al. (2001). Genotoxic assessments in aquatic environments under the influence of heavy metals and organic contaminants. Mutation Research, 490(2), 141–148.
Walker, G. C. (1985). Inducible DNA repair systems. Annual Review of Biochemistry, 54, 425–457.
Wilcox, P., & Williamson, S. (1986). Mutagenic activity of concentrated drinking water samples. Environmental Health Perspectives, 69, 141–150.
Zhou, B. B. , & Elledge, S. J. (2000). The DNA damage response: putting checkpoints in perspective. Nature, 408(6811), 433–439.
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Siddiqui, A.H., Tabrez, S. & Ahmad, M. Short-term in vitro and in vivo genotoxicity testing systems for some water bodies of Northern India. Environ Monit Assess 180, 87–95 (2011). https://doi.org/10.1007/s10661-010-1774-z
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DOI: https://doi.org/10.1007/s10661-010-1774-z