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Genetic interrelationships among medium to late maturing tropical maize inbred lines using selected SSR markers

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Abstract

Understanding the genetic relationships among breeding lines is fundamental in crop improvement programs. The objectives of this study were to apply selected polymorphic single sequence repeat (SSR) DNA markers and cluster medium to late maturing tropical elite maize inbred lines for effective hybrid breeding. Twenty elite inbred lines were genotyped with 20 SSR markers. The analysis detected a total of 108 alleles. The unweighted pair group method with arithmetic mean allocated the inbred lines into five clusters consistent with the known pedigrees. The tested inbred lines that were adapted to mid-altitude, sub-humid agro-ecologies were classified in different clusters, except for a few discrepancies. The greatest genetic distance was identified between the clusters of lines CML-202 and Gibe-1-91-1-1-1-1. The analysis determined the genetic grouping present in the source population, which will assist in effective utilization of the lines in tropical hybrid maize breeding programs to exploit heterosis.

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Acknowledgments

The Alliance for a Green Revolution in Africa (AGRA) is sincerely thanked for financial support to initiate the study. The Ethiopian Institute of Agricultural Research (EIAR) is acknowledged for providing leave of absence and hosting the field research to the first author. The all round support provided by the national maize research project of Ethiopia is highly appreciated.

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Authors and Affiliations

  1. Bako Research Center, P.O. Box 03, Bako, West Shoa, Ethiopia

    Abera Wende

  2. CIMMYT-Kenya ICRAF House, United Nations Avenue, Gigiri P.O. Box 1041, Village Market, Nairobi, 00621, Kenya

    Worku Mosisa

  3. African Center for Crop Improvement, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, Pietermaritzburg, South Africa

    Abera Wende, Hussein Shimelis, John Derera, Jedidah Danson & Mark D. Laing

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  1. Abera Wende
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  2. Hussein Shimelis
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  3. John Derera
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  4. Worku Mosisa
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  5. Jedidah Danson
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  6. Mark D. Laing
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Correspondence to Hussein Shimelis.

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Wende, A., Shimelis, H., Derera, J. et al. Genetic interrelationships among medium to late maturing tropical maize inbred lines using selected SSR markers. Euphytica 191, 269–277 (2013). https://doi.org/10.1007/s10681-012-0826-6

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  • DOI: https://doi.org/10.1007/s10681-012-0826-6

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