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Effects of dietary selenium on the pathological changes and oxidative stress in loach (Paramisgurnus dabryanus)

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Abstract

In this study, loach (Paramisgurnus dabryanus) were fed artificial diets containing 0.31 (control), 0.39, 0.48, 0.50 and 0.62 mg kg−1 of selenium (Se) for 60 days, respectively. Liver histopathology, hepatocyte ultrastructure, blood indices, biochemical parameters of liver functions and oxidative stress in the Se-treated loach were then assayed. The results showed the following: histopathological and ultrastructural lesions in liver were only observed in loach fed the 0.62 mg Se kg−1 diet; Haemoglobin and total protein were significantly increased in the 0.50 mg Se kg−1 group; albumin and high-density lipoprotein were increased significantly in the 0.48–0.50 mg Se kg−1 groups. However, white blood cell count was significantly decreased in the 0.48 mg Se kg−1 group; alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase were decreased in the 0.39–0.50 mg Se kg−1 groups. In liver tissue, the content of hydrogen peroxide was lower than that of controls in the 0.48–0.50 mg Se kg−1 groups, and the malondialdehyde level was lowest in the 0.48 mg Se kg−1 group. The activities of superoxide dismutase and glutathione peroxidase were significantly increased in the 0.50 mg Se kg−1 group; catalase and total antioxidant capacity were markedly increased in the 0.48–0.50 mg Se kg−1 group. These present results indicated that the dietary Se requirement for loach is 0.48–0.50 mg Se kg−1 diet.

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Acknowledgments

This work was supported by Science Fund of Jiangsu Province (BE2012354) and by Aquatic Three Project of Jiangsu Province (PJ2011-62, DY2012-3).

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  1. School of Preclinical Medicine and Life Science, Soochow University, 199, Renai Road, Suzhou, 215123, Jiangsu, People’s Republic of China

    Xiaofeng Hao, Qufei Ling & Fashui Hong

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  1. Xiaofeng Hao
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  2. Qufei Ling
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Correspondence to Qufei Ling.

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Hao, X., Ling, Q. & Hong, F. Effects of dietary selenium on the pathological changes and oxidative stress in loach (Paramisgurnus dabryanus). Fish Physiol Biochem 40, 1313–1323 (2014). https://doi.org/10.1007/s10695-014-9926-7

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