Abstract
The effects of NaCl salt (EC = 16 dS m−1) on water potential, and accumulation of proline, Na+ and K+ in leaves on the main stem of 30 wheat cultivars (Triticum aestivum L.) at awn appearance and 20 days after anthesis (20 DAA) were evaluated in a greenhouse experiment. Plants were arranged in a according to a randomized complete block design with factorial treatments in three replications. Proline accumulation at 20 DAA increased with increasing salt stress. This increase was 27.4-fold with the salt-sensitive cultivar “Ghods,” while the mean was 5.2-fold for 19 salt-resistant cultivars. Positive correlations between proline, and K+ + Na+ concentrations associated with higher sensitivity to salt stress indicated that proline may not have a protecting role against salt stress. No correlation was observed between leaf proline and water potential. Almost no contribution to the osmotic adjustment seems to be made by proline. The contribution made by proline to the osmotic adjustment of plants at 20 DAA was 0.69 bar, whereas that made by K+ and Na+ was 2.11 and 4.48 bar, respectively. The 30 wheat CVs used in this experiment showed different performances regarding the traits observed. Eleven of them showing the higher stress sensitivity indices had the highest level of proline and Na+ concentrations. They were considered to be salt-sensitive cultivars. Among the others, nine cultivars showed salt tolerance with almost the same Na+ and proline concentrations, but a higher K+/Na+ selectivity of ions from leaf to grains. In 10 of the cultivars, Na+ and proline concentrations were low, indicating the presence of a salt avoiding mechanism.
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References
Ashraf M, Harris PJC (2004) Potential biochemical indicators of salt tolerance in plants. Plant Sci 166:3–16
Aziz A, Martin-Tanguy J, Larher F (1998) Stress-induced changes in polyamine and tyramine levels can regulate praline accumulation in tomato leaf discs treated with sodium chloride. Physiol Plant 104:195–202
Bajji M, Lutts S, Kinet JM (2001) Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Sci 160:669–681
Bates LSRP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
Chaudhary MT, Merrett MJ, Wainwright SJ (1997) Growth, ion content and proline accumulation in NaCl-selected and non-selected cell lines of Lucerne cultured on sodium and potassium salts. Plant Sci 127:71–79
Chen DM, Keiper FJ, De Filippis LF (1998) Physiological changes accompanying the induction of salt tolerance in Eucalyptus microcorys shoots in tissue. J Plant Physiol 152:555–563
Chhipa BR, Lal P (1995) Na/K ratios as the basis of salt tolerance in wheat. Aust J Agric Res 46:533–539
Chu TM, Aspinal D, Paleg LG (1976) Stress metabolism VII: salt and proline accumulation in barley. Aust J Plant Physiol 3:219–222
Clark JM, Townley-Smith TF, McCaig TN, Green DG (1984) Growth analysis of spring wheat cultivars of varying drought resistance. Crop Sci 24:537–541
Colmer TD, Epstein E, Dvorak J (1995) Differential solute regulation in leaf blades of various ages in salt-sensitive wheat and a salt tolerant wheat * Lophopyrum elongatum (Host) Löve, A. amphiploid. Plant Physiol 108:1715–1724
Colmer TD, Fan TW, Higashi RM, Lauchli A (1996) Interactive effects of Ca2+ and NaCl salt on the ionic relations and proline accumulation in the primary root tip of Sorghum bicolor. Physiol Plant 97:421–424
Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Ann Rev Plant Physiol 31:149–190
Gupta S, Srivastava J (1990) Effect of salt stress on morphophysiological parameters in wheat. Indian J Plant Physiol 32:162–171
Gupta SD, Auge RM, Denchev PD, Conger BV (1995) Growth, proline accumulation and water relations of NaCl-selected and non-selected callus lines of Dactylis glomerata. Environ Exp Bot 35:83–92
Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular response to high salt. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
Heuer B (1995) Osmoregulatory role of proline in water and salt stressed plants. In: Pessarakli M (ed) Handbook of plant and crop stress. London, pp 363–381
Kemble AR, Mac Pherson HT (1954) Liberation of amino acids in perennial rye grass during wilting. Biochem J 58:46–54
Khodary SEA (1992) Effect of salt and tryptophan on growth and some metabolic changes in wheat and sorghum plants. Biol Plant 34:439–443
Kingsbury RW, Epstein E, Pearcy RW (1984) Physiological responses to salt in selected lines of wheat. Plant Physiol 74:417–423
Lacerda CF, Cambraia J, Oliva MA, Ruiz HA (2005) Changes in growth and in solute concentrations in sorghum leaves and roots during salt stress recovery. Environ Exp Bot 54:69–76
Lutts S, Kinet JM, Bouharmont J (1996) Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice cultivars differing in salt resistance. Plant Growth Reg 19:207–218
Maggio A, Reddy MP, Joly RJ (2000) Leaf gas exchange and solute accumulation in the halophyte Salvadora persica grown at moderate salt. Environ Exp Bot 44:31–38
Mansour MMF (1998) Protection of plasma membrane of onion epidermal cells by glycinebetaine and proline against NaCl stress. Plant Physiol Biochem 36:767–772
Naidu BP, Paleg LG, Aspinall D, Jennings AC, Jones GP (1990) Rate of imposition of water stress alters the accumulation of nitrogen-containing solutes by wheat seedlings. Aust J Plant Physiol 17:653–664
Poustini K, Siosemardeh A (2001) K+/Na+ ratio and ion selectivity in response to salt stress in wheat. Iran J Agric Sci 32:525–532
Reynoso GT, Gamboa BA (1982) Salt tolerance in the freshwater alga Chlamydomonas reinhardii: effect of proline and taurine. Comp Biochem Physiol 73:95–99
Rout NP, Shaw BP (1998) Salt tolerance in aquatic macrophytes: probable role of proline, the enzymes involved in its synthesis and C4 type of metabolism. Plant Sci 136:121–130
Sairam RK, Rao KV, Srivastava GC (2002) Differential response of wheat genotypes to longterm salt stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037–1046
Saradhi A, Saradhi PP (1991) Proline accumulations under heavy metal stress. J Plant Physiol 138:554–558
Shannon MC (1984) Breeding selection and the genetic of salt tolerance. In: Staples RC, Toenniessen GH (eds) Salt tolerance in plants. John Wiley, New York, pp 231–251
Shevyakova NI, Rakitian VY, Muzychko LM, Kuznetzov VV (1998) Stress-induced accumulation of proline in relation to salt tolerance of intact plants and isolated cells. Appl Biochem Microb 34:291–295
Suiyun C, Guangmin X, Taiyong Q, Fengnin X, Yan J, Huimin C (2004) Introgression of salt-tolerance from somatic hybrids between common wheat and Thinopyrum ponticum. Plant Sci 167:773–779
Zhul X, Gong H, Chen G, Wang S, Zhang C (2005) Different solute levels in two spring wheat cultivars induced by progressive field water stress at different developmental stages. J Arid Environ 62:1–14
Acknowledgements
This research project has been supported by Grant No. NRCI 690 from the National Research Council of the Islamic Republic of Iran. This support is highly appreciated.
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Poustini, K., Siosemardeh, A. & Ranjbar, M. Proline accumulation as a response to salt stress in 30 wheat (Triticum aestivum L.) cultivars differing in salt tolerance. Genet Resour Crop Evol 54, 925–934 (2007). https://doi.org/10.1007/s10722-006-9165-6
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DOI: https://doi.org/10.1007/s10722-006-9165-6