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Role of some growth regulators on cytogenetic activity of barley under salt stress

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Abstract

The effects of gibberellic acid (GA3), kinetin (KIN), benzyladenine and ethylene (E) on mitotic activity and chromosomal aberrations in root tips of barley seeds (Hordeum vulgare L. cv. “Bülbül 89”) germinated under salt stress were investigated. It was determined that all of these plant growth regulators (PGRs) decreased mitotic index in root tips of barley seeds germinated at 20 °C and in distilled water. Furthermore, some of the PGRs studied increased significantly the frequency of chromosomal aberrations. The frequency of chromosomal aberrations in seeds treated with E and KIN was considerably higher than in the seeds germinated under nonstress conditions. The inhibitory effect of salt stress on mitotic index increased with increasing salt concentration (0.30, 0.35, 0.40 and 0.45 molal, m). GA3 and KIN pretreatments showed a successful performance in ameliorating the negative effects of increasing salinity on mitotic activity. The number of chromosomal aberrations also increased with increasing NaCl concentration. However, most of the PGR pretreatments studied alleviated the detrimental effects of increasing salinity on chromosomal aberrations. KIN pretreatment at 0.30 and 0.35 m salinity could not rescued the cytogenetic activity of salt stress on this parameter.

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Acknowledgments

The authors thank the Department of Scientific Research Project Management of Süleyman Demirel University (SDUBAP) for the financial support of the project SDUBAP (1167-YL-05). Thanks also to Assist. Prof. Dr. Kürşat Çavuşoğlu (Süleyman Demirel Univ., Biology Department, Isparta, Türkiye) for his help in a preliminary study.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Süleyman Demirel University, 32100, Isparta, Turkey

    Selma Tabur

  2. Directorate of Provincial Environment and Forestry, 64100, Uşak, Turkey

    Kıymet Demir

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  1. Selma Tabur
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  2. Kıymet Demir
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Correspondence to Selma Tabur.

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Tabur, S., Demir, K. Role of some growth regulators on cytogenetic activity of barley under salt stress. Plant Growth Regul 60, 99–104 (2010). https://doi.org/10.1007/s10725-009-9424-6

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