Abstract
Background and Objective
Low level laser therapy (LLLT) is a known anti-inflammatory therapy. Herein we studied the effect of LLLT on lung permeability and the IL-1β level in LPS-induced pulmonary inflammation.
Study Design/Methodology
Rats were divided into 12 groups (n = 7 for each group). Lung permeability was measured by quantifying extravasated albumin concentration in lung homogenate, inflammatory cells influx was determined by myeloperoxidase activity, IL-1β in BAL was determined by ELISA and IL-1β mRNA expression in trachea was evaluated by RT-PCR. The rats were irradiated on the skin over the upper bronchus at the site of tracheotomy after LPS.
Results
LLLT attenuated lung permeability. In addition, there was reduced neutrophil influx, myeloperoxidase activity and both IL-1β in BAL and IL-1β mRNA expression in trachea obtained from animals subjected to LPS-induced inflammation.
Conclusion
LLLT reduced the lung permeability by a mechanism in which the IL-1β seems to have an important role.
Similar content being viewed by others
References
Kips, J. C., J. Tavernier, and R. A. Pauwels. 1992. Tumor necrosis factor causes bronchial hyperresponsiveness in rats. Am. Rev. Respir. Dis. 145:332–336.
Miller, D. L., K. Welty-Wolf, M. S. Carraway, M. Ezban, A. Ghio, H. Suliman, and C. A. Piantadosi. 2002. Extrinsic coagulation blockade attenuates lung injury and pro-inflammatory cytokine release after intratracheal lipopolysaccharide. Am. J. Respir. Cell Mol. Biol. 26(6):650–658.
Jansson, A. H., C. Eriksson, and X. Wang. 2004. Lung inflammatory responses and hyperinflation induced by an intratracheal exposure to lipopolysaccharide in rats. Lung 182(3):163–171.
Lappalainen, U., J. A. Whitsett, S. E. Wert, J. W. Tichelaar, and K. Bry. 2005. Interleukin-1b causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung. Am. J. Respir. Cell Mol. Biol. 32:311–318.
Johnson, V. J., B. Yucesoy, and M. I. Luster. 2005. Prevention of IL-1 signaling attenuates airway hyperresponsiveness and inflammation in a murine model of toluene diisocyanate-induced asthma. J. Allergy Clin. Immunol. 116(4):851–858.
Wang, C. C., C. L. Fu, Y. H. Yang, Y. C. Lo, L. C. Wang, Y. H. Chuang, and D. M. Chiang. 2006. Adenovirus expressing interleukin-1 receptor antagonist alleviates allergic airway inflammation in a murine model of asthma. Gene Ther. 13(19):1414–1421.
Rose, C. E. Jr, C. A. Juliano, D. E. Tracey, T. Yoshimura, and S. M. Fu. 1994. Role of interleukin-1 in endotoxin-induced lung injury in the rat. Am. J. Respir. Cell Mol. Biol. 10(2):214–221.
Warren, J. S. 1992. Relationship between interleukin-1 beta and platelet-activating factor in the pathogenesis of acute immune complex alveolitis in the rat. Am. J. Pathol. 141(3):551–560.
Ware, L. B., and M. A. Matthay. 2000. The acute respiratory distress syndrome. N. Engl. J. Med. 342:1334–1349.
Bachofen, M., and E. R. Weibel. 1982. Structural alterations of lung parenchyma in the adult respiratory syndrome. Clin. Chest Med. 3:35–56.
Dudek, S. M., and J. G. N. Garcia. 2001. Cytoskeleton regulation of pulmonary vascular permeability. J. Appl. Physiol. 91:1487–1500.
Kao, S. J., D. D. Liu, C. F. Su, and H. I. Chen. 2007. Niacinamide abrogates the organ dysfunction and acute lung injury caused by endotoxin. J. Cardiovasc. Pharmacol. 50(3):333–342.
Frank, J. A., J. F. Pittet, C. Wray, and M. A. Matthay. 2008. Protection from experimental ventilator-induced acute lung injury by IL-1 receptor blockade. Thorax. 63:147–153.
Cazzola, M., and R. Polosa. 2006. Anti-TNF-alpha and Th1 cytokine-directed therapies for the treatment of asthma. Curr. Opin. Allergy Clin. Immunol. 6(1):43–50.
Stirling, R. J., and K. F. Chung. 2000. Future treatments of allergic diseases and asthma. Br. Med. Bull. 56(4):1037–1053.
Bjordal, J. M., M. I. Johnson, V. Iversen, F. Aimbire, and R. A. B. Lopes-Martins. 2006. Low Level Laser Therapy (LLLT) and acute pain: A systematic review of possible mechanisms of action and clinical effects in randomized placebo-controlled trials. Photomed. Laser Surg. 24(2):160–170.
Milojevic, M., and V. Kuruc. 2004. The role of physical rehabilitation in the treatment of exudative pleurisy. Med. Pregl. 57(1–2):13–17.
Landyshev, I. U. S., N. V. Avdeeva, N. D. Goborov, N. P. Krasavina, G. A. Tikhonova, and S. I. Tkacheva. 2002. Efficacy of low intensity laser irradiation and sodium nedocromil in the complex treatment of patients with bronchial asthma. Ter. Arkh. 74(3):25–28.
Aimbire, F., R. A. B. Lopes-Martins, R. Albertini, M. T. T. Pacheco, H. C. Castro-Faria-Neto, P. S. L. M. Leonardo, and J. M. Bjordal. 2007. Effect of Low-Level Laser therapy on hemorrhagic lesions by immune complex in rat lungs. Photomed. and Laser Surg. 25(2):112–117.
Aimbire, F., J. M. Bjordal, V. V. Iversen, R. Albertini, L. Frigo, M. T. Pacheco, H. C. Castro-Faria-Neto, M. C. Chavantes, R. M. Labat, and R. A. Lopes-Martins. 2006. Low level laser therapy partially restores trachea muscle relaxation response in rats with tumor necrosis factor alpha-mediated smooth airway muscle dysfunction. Lasers Surg. Med. 38(8):773–778.
Akeson, A. L., C. W. Woods, L. C. Hsieh, R. A. Bohnke, B. L. Ackermann, K. Y. Chan, J. L. Robinson, S. D. Yanofsky, J. W. Jacobs, R. W. Barrett, and T. L. Bowlin. 1996. AF12198, a novel low molecular weight antagonist, selectively binds the human type I interleukin (IL)-1 receptor and blocks in vivo responses to IL-1. J. Biol. Chem. 271(48):30517–30523.
Shimada, H., N. Hasegawa, H. Koh, S. Tasaka, M. Shimizu, W. Yamada, T. Nishimura, K. Amakawa, M. Kohno, M. Sawafuji, K. Nakamura, S. Fujishima, K. Yamaguchi, and A. Ishizaka. 2006. Effects of initial passage of endotoxin through the liver on the extent of acute lung injury in a rat model. Shock 26(3):311–315.
Wang, L. F., M. Patel, H. Razavi, S. Weicker, M. Joseph, D. McCormack, and S. Mehta. 2002. Role of inducible nitric oxide synthase in pulmonary microvascular protein leak in murine sepsis. Am. J. Respir. Crit. Care Med. 165:1634–1639.
Tavares de Lima, W., A. A. Steil, M. Russo, N. Starobinas, C. F. Teixeira, and S. Jancar. 1998. Lipid mediators, tumor necrosis factor and nitric oxide and their interactions in immune-complex-induced lung injury. Eur. J. Pharmacol. 358(1):69–75.
Goldblum, S. E., K. M. Wu, and M. Jay. 1985. Lung myeloperoxidase as a measure pf pulmonary leukostasis in rabbits. J. Appl. Physiol. 59:1978–1985.
Dinarello, C. A. 1996. Biologic basis of interleukin-1 in disease. Blood 87(6):2095–2147.
Borish, L., J. J. Mascali, J. Dishuck, W. R. Beam, R. J. Martin, and L. J. Rosenwasser. 1992. Detection of alveolar macrophage-derived IL-1b in asthma. Inhibition with corticosteroids. J. Immunol. 149:3340–3346.
Peng, X., P. M. Hassoun, S. Sammani, B. J. McVerry, M. J. Burne, H. Rabb, D. Pearse, R. M. Tuder, and J. G. N. Garcia. 2004. Protective effects of sphingosine 1-phosphate in murine endotoxin-induced inflammatory lung injury. Am. J. Respir. Crit. Care Med. 169:1245–1251.
Gerlach, H., D. Keh, A. Semmerow, T. Busch, K. Lewandowski, D. M. Pappert, R. Rossaint, and K. J. Falke. 2003. Dose–response characteristics during long-term inhalation of nitric oxide in patients with severe acute respiratory distress syndrome: a prospective, randomized, controlled study. Am. J. Respir. Crit. Care Med. 167(7):1008–1015.
Pilkington, C. A., and L. R. Weddenburg. 2005. Pediatric idiopathic inflammatory muscle disease: recognition and management. Drugs 65(10):1355–1365.
Chung, F. 2001. Anti-inflammatory cytokines in asthma and allergy: interleukin-10, interleukin-12, interferon-g. Mediat. Inflamm. 10:51–59.
Alagappan, V. K., S. McKay, A. Widyastuti, I. M. Garrelds, A. J. Bogers, H. C. Hoogsteden, S. J. Hirst, and H. S. Sharma. 2005. Pro-inflammatory cytokines upregulate mRNA expression and secretion of vascular endothelial growth factor in cultured human airway smooth muscle cells. Cell Biochem. Biophys. 43(1):119–129.
Kupfner, J. G., J. J. Arcaroli, H. K. Yum, S. G. Nadler, K. Y. Yang, and E. Abraham. 2001. Role of NF-kappaB in endotoxemia-induced alterations of lung neutrophil apoptosis. J. Immunol. 167(12):7044–7051.
Niwa, M., A. Hara, Y. Kanamori, D. Hatakeyama, M. Saio, T. Takami, H. Matsuno, O. Kozawa, and T. Uematsu. 2000. Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis. Eur. J. Pharmacol. 407(3):211–219.
Coras, B., U. Hohenleutner, M. Landthaler, and S. Hohenleutner. 2005. Early recurrence of eruptive vellus hair cysts after Er:YAG laser therapy: case report and review of the literature. Dermatol. Surg. 31(12):1741–1744.
Witman, P. M., A. M. Wagner, K. Scherer, M. Waner, and I. J. Frieden. 2006. Complications following pulsed dye laser treatment of superficial hemangiomas. Lasers Surg. Med. 38(2):116–123.
Lee, H. W., S. J. Ahn, M. W. Lee, J. H. Choi, K. C. Moon, and J. K. J. Koh. 2006. Pseudomelanoma following laser therapy. Eur. Acad. Dermatol. Venereol. 20(3):342–344.
Chan, H. H., C. H. Yang, J. C. Leung, W. I. Wei, and K. N. Lai. 2007. An animal study of the effects on p16 and PCNA expression of repeated treatment with high-energy laser and intense pulsed light exposure. Lasers Surg. Med. 39(1):8–13.
Dinarello, C. A. 2005. Interkeukin-1b. Crit. Care Med. 33(12):460–462.
Albertini, R., F. Aimbire, A. B. Villaverde, J. A. Silva Jr, and M. S. Costa. 2007. COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy. Inflamm. Res. 56:1–2.
Acknowledgements
The authors acknowledge the FAPESP, for the grants (04/10539-5 and 05/54145-3), under which this research was conducted.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Aimbire, F., Ligeiro de Oliveira, A.P., Albertini, R. et al. Low Level Laser Therapy (LLLT) Decreases Pulmonary Microvascular Leakage, Neutrophil Influx and IL-1β Levels in Airway and Lung from Rat Subjected to LPS-Induced Inflammation. Inflammation 31, 189–197 (2008). https://doi.org/10.1007/s10753-008-9064-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-008-9064-4