Skip to main content

Advertisement

SpringerLink
Log in

Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Carbon nanotubes (CNTs) have been one of the most extensively researched and developed nanomaterials. However, little concern has been placed on their safety. The biological effects of CNTs are believed to differ relative to size and shape. Thus, the relationship between the characteristics of CNTs and their safety needs to be evaluated. In this study, we examined the biological effects of different-sized multi-walled CNTs (MWCNTs) and single-walled CNTs (SWCNTs). Long and thick MWCNTs induced the strongest DNA damage while similar SWCNTs caused little effect. Comparison of inflammatory responses of various types of CNTs found that peritoneal CNT administration of long and thick MWCNTs increased the total cell number in abdominal lavage fluid in mice. These results indicate that long and thick MWCNT, but not short and thin MWCNT, cause DNA damage and severe inflammatory effects. These findings might provide useful information for constructing novel CNTs with safety.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Sternand, S.T., and S.E. McNeil. 2008. Nanotechnology safety concerns revisited. Toxicol Sci 101: 4–21.

    Article  Google Scholar 

  2. Lacerda, L., A. Bianco, M. Prato, and K. Kostarelos. 2006. Carbon nanotubes as nanomedicines: From toxicology to pharmacology. Adv Drug Deliv Rev 58: 1460–1470.

    Article  CAS  PubMed  Google Scholar 

  3. Tran, P., L. Zhang, and T.J. Webster. 2009. Carbon nanofibers and carbon nanotubes in regenerative medicine. Adv Drug Deliv Rev 61: 1097–1114.

    Article  CAS  PubMed  Google Scholar 

  4. Donaldson, K., R. Aitken, L. Tran, V. Stone, R. Duffin, G. Forrest, and A. Alexander. 2006. Carbon nanotubes: A review of their properties in relation to pulmonary toxicology and workplace safety. Toxicol Sci 92: 5–22.

    Article  CAS  PubMed  Google Scholar 

  5. Maynard, A.D. 2007. Nanotechnology: The next big thing, or much ado about nothing? Ann Occup Hyg 51: 1–12.

    Article  CAS  PubMed  Google Scholar 

  6. Kaneand, A.B., and R.H. Hurt. 2008. Nanotoxicology: The asbestos analogy revisited. Nat Nanotechnol 3: 378–379.

    Google Scholar 

  7. Shvedova, A.A., E.R. Kisin, D. Porter, P. Schulte, V.E. Kagan, B. Fadeel, and V. Castranova. 2009. Mechanisms of pulmonary toxicity and medical applications of carbon nanotubes: Two faces of Janus? Pharmacol Ther 121: 192–204.

    Article  CAS  PubMed  Google Scholar 

  8. Poland, C.A., R. Duffin, I. Kinloch, A. Maynard, W.A. Wallace, A. Seaton, V. Stone, S. Brown, W. Macnee, and K. Donaldson. 2008. Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nat Nanotechnol 3: 423–428.

    Article  CAS  PubMed  Google Scholar 

  9. Takagi, A., A. Hirose, T. Nishimura, N. Fukumori, A. Ogata, N. Ohashi, S. Kitajima, and J. Kanno. 2008. Induction of mesothelioma in p53+/- mouse by intraperitoneal application of multi-wall carbon nanotube. J Toxicol Sci 33: 105–116.

    Article  CAS  PubMed  Google Scholar 

  10. Sakamoto, Y., D. Nakae, N. Fukumori, K. Tayama, A. Maekawa, K. Imai, A. Hirose, T. Nishimura, N. Ohashi, and A. Ogata. 2009. Induction of mesothelioma by a single intrascrotal administration of multi-wall carbon nanotube in intact male Fischer 344 rats. J Toxicol Sci 34: 65–76.

    Article  CAS  PubMed  Google Scholar 

  11. Shibata, H., Y. Yoshioka, A. Ohkawa, K. Minowa, Y. Mukai, Y. Abe, M. Taniai, T. Nomura, H. Kayamuro, H. Nabeshi, T. Sugita, S. Imai, K. Nagano, T. Yoshikawa, T. Fujita, S. Nakagawa, A. Yamamoto, T. Ohta, T. Hayakawa, T. Mayumi, P. Vandenabeele, B.B. Aggarwal, T. Nakamura, Y. Yamagata, S. Tsunoda, H. Kamada, and Y. Tsutsumi. 2008. Creation and X-ray structure analysis of the tumor necrosis factor receptor-1-selective mutant of a tumor necrosis factor-alpha antagonist. J Biol Chem 283: 998–1007.

    Article  CAS  PubMed  Google Scholar 

  12. Maynard, S., S.H. Schurman, C. Harboe, N.C. de Souza-Pinto, and V.A. Bohr. 2009. Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis 30: 2–10.

    Article  CAS  PubMed  Google Scholar 

  13. Mossmanand, B.T., and A. Churg. 1998. Mechanisms in the pathogenesis of asbestosis and silicosis. Am J Respir Crit Care Med 157: 1666–1680.

    Google Scholar 

  14. Kampand, D.W., and S.A. Weitzman. 1999. The molecular basis of asbestos induced lung injury. Thorax 54: 638–652.

    Article  Google Scholar 

  15. Pollard, J.W. 2004. Tumour-educated macrophages promote tumour progression and metastasis. Nat Rev Cancer 4: 71–78.

    Article  CAS  PubMed  Google Scholar 

  16. Clevers, H. 2004. At the crossroads of inflammation and cancer. Cell 118: 671–674.

    Article  CAS  PubMed  Google Scholar 

  17. Krelin, Y., E. Voronov, S. Dotan, M. Elkabets, E. Reich, M. Fogel, M. Huszar, Y. Iwakura, S. Segal, C.A. Dinarello, and R.N. Apte. 2007. Interleukin-1beta-driven inflammation promotes the development and invasiveness of chemical carcinogen-induced tumors. Cancer Res 67: 1062–1071.

    Article  CAS  PubMed  Google Scholar 

  18. Dostert, C., V. Petrilli, R. Van Bruggen, C. Steele, B.T. Mossman, and J. Tschopp. 2008. Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science 320: 674–677.

    Article  CAS  PubMed  Google Scholar 

  19. Cassel, S.L., S.C. Eisenbarth, S.S. Iyer, J.J. Sadler, O.R. Colegio, L.A. Tephly, A.B. Carter, P.B. Rothman, R.A. Flavell, and F.S. Sutterwala. 2008. The Nalp3 inflammasome is essential for the development of silicosis. Proc Natl Acad Sci U S A 105: 9035–9040.

    Article  CAS  PubMed  Google Scholar 

  20. Mantovani, A., P. Allavena, A. Sica, and F. Balkwill. 2008. Cancer-related inflammation. Nature 454: 436–444.

    Article  CAS  PubMed  Google Scholar 

  21. Grivennikov, S.I., and M. Karin. 2009. Inflammation and oncogenesis: a vicious connection. Curr Opin Genet Dev. doi:10.1016/j.gde.2009.11.004.

Download references

Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and from the Japan Society for the Promotion of Science (JSPS). This study was also supported in part by Health Labor Sciences Research Grants from the Ministry of Health, Labor, and Welfare of Japan; by Health Sciences Research Grants for Research on Health Sciences focusing on Drug Innovation from the Japan Health Sciences Foundation; by a Grant from the Minister of the Environment; and by The Nagai Foundation Tokyo.

Author information

Authors and Affiliations

  1. Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan

    Kohei Yamashita, Yasuo Yoshioka, Kazuma Higashisaka, Yuki Morishita, Tokuyuki Yoshida, Maho Fujimura, Hiroyuki Kayamuro, Hiromi Nabeshi, Takuya Yamashita, Tomoaki Yoshikawa, Norio Itoh, Shin-ichi Tsunoda & Yasuo Tsutsumi

  2. Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation (NiBio), 7-6-8 Saito-asagi, Ibaraki, Osaka, 567-0085, Japan

    Kohei Yamashita, Yasuo Yoshioka, Kazuma Higashisaka, Yuki Morishita, Tokuyuki Yoshida, Maho Fujimura, Hiroyuki Kayamuro, Hiromi Nabeshi, Takuya Yamashita, Kazuya Nagano, Yasuhiro Abe, Haruhiko Kamada, Tomoaki Yoshikawa, Shin-ichi Tsunoda & Yasuo Tsutsumi

  3. The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan

    Yasuo Yoshioka, Haruhiko Kamada, Shin-ichi Tsunoda & Yasuo Tsutsumi

  4. Graduate School of Pharmaceutical Sciences, Kobegakuin University, 1-1-3, Minatojima, Chuo-ku, Kobe, 650-8586, Japan

    Yuichi Kawai & Tadanori Mayumi

Authors
  1. Kohei Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuo Yoshioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuma Higashisaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuki Morishita
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tokuyuki Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maho Fujimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroyuki Kayamuro
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiromi Nabeshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Takuya Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Kazuya Nagano
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Yasuhiro Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Haruhiko Kamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Yuichi Kawai
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Tadanori Mayumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Tomoaki Yoshikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Norio Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. Shin-ichi Tsunoda
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. Yasuo Tsutsumi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yasuo Yoshioka, Shin-ichi Tsunoda or Yasuo Tsutsumi.

Additional information

Kohei Yamashita and Yasuo Yoshioka contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamashita, K., Yoshioka, Y., Higashisaka, K. et al. Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape. Inflammation 33, 276–280 (2010). https://doi.org/10.1007/s10753-010-9182-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-010-9182-7

KEY WORDS

Search

Navigation