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The released polysaccharide of the cyanobacterium Aphanothece halophytica inhibits flocculation of the alga with ferric chloride

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Abstract

The effect of the released polysaccharide (RPS) of the cyanobacterium Aphanothece halophytica GR02 on the recovery of the alga by flocculation with ferric chloride was studied. With increasing RPS concentration in algal cultures from 0 to 68 mg L−1 the flocculation efficiency at the same dosage of ferric chloride decreased, and higher dosages of ferric chloride were required to attain the same flocculation efficiency. It is demonstrated that RPS could form complexes with ferrum during flocculation. In conclusion, RPS of A. halophytica GR02 had a significant inhibitory effect on flocculation of the alga with ferric chloride. The inhibitory mechanism of A. halophytica GR02 RPS allows the RPS to compete for ferrum by forming complexes with ferrum, thus leading to the consumption of ferrum in ferric chloride.

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References

  • Bernhardt H, Hoyer O, Shell H, Lusse B (1985) Reaction mechanisms involved in the influence of algogenic organic matter on flocculation. Z Wasser-Abwasser-Forch 18:18–30

    CAS  Google Scholar 

  • Bernhardt H, Hoyer O, Lusse B, Shell H (1987) Investigations on the influence of algal-derived organic substances on flocculation and filtration. In: Huck PM, Toft P (eds) Treatment of drinking water of organic contaminants. Pergamon, London, pp 185–216

    Google Scholar 

  • Borowitzka MA (1988) Algal growth media and sources of algal cultures. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp 456–465

    Google Scholar 

  • Buelna G, Bhattarai KK, de la Noüe J, Taiganides EP (1990) Evaluation of various flocculants for the recovery of algal biomass growth on pig-waste. Biol Wastes 31:211–222, doi:10.1016/0269-7483(90)90160-T

    Article  CAS  Google Scholar 

  • Cheng WP, Chi FH (2003) Influence of eutrophication on the coagulation efficiency in reservoir water. Chemosphere 53:773–778, doi:10.1016/S0045-6535(03)00510-1

    Article  PubMed  CAS  Google Scholar 

  • D’Souza FL, Knuckey RM, Hohmann S, Pendrey RC (2002) Flocculated microalgae concentrates as diets for larvae of the tiger prawn Penaeus monodon Fabricius. Aquacult Nutr 8:113–120, doi:10.1046/j.1365-2095.2002.00198.x

    Article  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method of determination of sugars and related substances. Anal Chem 28:350–356, doi:10.1021/ac60111a017

    Article  CAS  Google Scholar 

  • Gregor JE, Fenton E, Brokenshire G, Brink PVD, O’Sullivan B (1996) Interactions of calcium and aluminium ions with alginate. Water Res 30:1319–1324, doi:10.1016/0043-1354(95)00194-8

    Article  CAS  Google Scholar 

  • Grima EM, Belarbi E-H, Fernández A, Medina AR, Chisti Y (2003) Recovery of microalgal biomass and metabolites: process options and economics. Biotechnol Adv 20:291–315, doi:10.1016/S0734-9750(02)00050-2

    Google Scholar 

  • Gudin C, Therpenier C (1986) Bioconversion of solar energy into organic chemicals by microalgae. Adv Biotechnol Proc 6:73–110

    CAS  Google Scholar 

  • Hilditch CM, Smith AJ, Balding P, Rogers L (1991) C-phycocyanin from the cyanobacterium Aphanothece halophytica. Phytochemistry 30:3515–3517, doi:10.1016/0031-9422(91)80057-8

    Article  CAS  Google Scholar 

  • Knuckey RM, Brown MR, Robert R, Frampton DMF (2006) Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds. Aquacult Eng 35:300–313, doi:10.1016/j.aquaeng.2006.04.001

    Article  Google Scholar 

  • Li PF, Liu ZL, Xu R (2001) Chemical characterisation of the released polysaccharide from the cyanobacterium Aphanothece halophytica GR02. J Appl Phycol 13:71–77, doi:10.1023/A:1008109501066

    Article  Google Scholar 

  • Li WS, Men X, Li PF, Chen L, Liu ZL (in press) Extraction, purification and characterization of phycocyanin from Aphanothece halophytica. J Salt Chem Ind (in Chinese with English abstract)

  • Morineau-Thomas O, Jaouen P, Legentilhomme P (2002) The role of exopolysaccharides infouling phenomenon during ultrafiltration of microalgae (Chlorella sp. and Porphyridium purpureum): advantage of a swirling decaying flow. Bioprocess Biosyst Eng 25:35–42, doi:10.1007/s00449-001-0278-1

    Article  PubMed  CAS  Google Scholar 

  • Morris GA, Li P, Puaud M, Liu Z, Mitchell JR, Harding SE (2001) Hydrodynamic characterisation of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: a comparison with xanthan. Carbohydr Polym 44:261–268, doi:10.1016/S0144-8617(00)00217-4

    Article  CAS  Google Scholar 

  • Takaara T, Sano D, Konno H, Omura T (2007) Cellular proteins of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride. Water Res 41:1653–1658, doi:10.1016/j.watres.2007.01.035

    Article  PubMed  CAS  Google Scholar 

  • Yopp JH, Tindall DR, Miller DM, Schmid WE (1978) Isolation, purification and evidence for a halophilic nature of the blue-green alga Aphanthece halophytica Frémy (Chroococcales). Phycologia 17:172–178

    CAS  Google Scholar 

Download references

Acknowledgements

This study was sponsored by the National Natural Science Foundation of China (No. 30670400) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.

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Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, People’s Republic of China

    Li Chen, Pengfu Li, Zhili Liu & Qingcai Jiao

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  1. Li Chen
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  2. Pengfu Li
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  3. Zhili Liu
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  4. Qingcai Jiao
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Correspondence to Pengfu Li.

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Chen, L., Li, P., Liu, Z. et al. The released polysaccharide of the cyanobacterium Aphanothece halophytica inhibits flocculation of the alga with ferric chloride. J Appl Phycol 21, 327–331 (2009). https://doi.org/10.1007/s10811-008-9371-z

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  • DOI: https://doi.org/10.1007/s10811-008-9371-z

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