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The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures

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Abstract

The addition of bicarbonate (NaHCO3; 0, 1, or 2 g L−1) to microalgal cultures has been evaluated for two species (Tetraselmis suecica and Nannochloropsis salina) in respect of growth and biochemical composition. In batch cultures, addition of bicarbonate (1 g L−1) resulted in significantly (P < 0.05) higher final mean cell abundances for both species. No differences in specific growth rates (SGRs) were recorded for T. suecica between treatments; however, increasing bicarbonate addition decreased SGR values in N. salina cultures. Bicarbonate addition (1 g L−1) significantly improved nitrate utilisation from the external media and photosynthetic efficiency (F v /F m ) in both species. For both T. suecica and N. salina, bicarbonate addition significantly increased the cellular concentrations of total pigments (3,432–3,587 and 19–37 fg cell−1, respectively) compared to cultures with no additional bicarbonate (1,727 and 11 fg cell−1, respectively). Moreover, final concentrations of total cellular fatty acids in T. suecica and N. salina cultures supplemented with 2 g L−1 bicarbonate (7.6 ± 1.2 and 1.8 ± 0.1 pg cell−1, respectively) were significantly higher than those cells supplemented with 0 or 1 g L−1 bicarbonate (3.2–3.5 and 0.9–1.0 pg cell−1, respectively). In nitrate-deplete cultures, bicarbonate addition caused species-specific differences in the rate of cellular lipid production, rates of change in fatty acid composition and final lipid levels. In summary, the addition of sodium bicarbonate is a viable strategy to increase cellular abundance and concentrations of pigments and lipids in some microalgae as well as the rate of lipid accumulation in nitrate-deplete cultures.

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Acknowledgments

The authors would like to thank Helen Findlay and Victor Martinez-Vincente for their assistance in the calculation of DIC species and pigment analyses, respectively. This work was funded by the UK's Carbon Trust ‘Algal Biofuels Challenge’ programme.

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  1. Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, Devon, PL1 3DH, UK

    D. A. White, A. Pagarette, P. Rooks & S. T. Ali

  2. Department of Biology, University of Bergen, Thormøhlensgate 53 A & B, 5006, Bergen, Norway

    A. Pagarette

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White, D.A., Pagarette, A., Rooks, P. et al. The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures. J Appl Phycol 25, 153–165 (2013). https://doi.org/10.1007/s10811-012-9849-6

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