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The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation

  • Fertility Preservation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Reducing the ischemic damage from free radicals that is inflicted on ovarian tissue is critical for successful ovarian tissue transplantation. Polyethylene glycol-superoxide dismutase (PEG-SOD) is mimetic of superoxide dismutase (SOD) and powerful free radical scavenger acts by reducing superoxide anions. The objective of study was to evaluate effects of PEG-SOD on mouse ovarian tissues in in vitro culture and in autotransplantation.

Methods

Ovaries were collected and randomly divided into four groups that received different doses of PEG-SOD. To assess effects of PEG-SOD on in vitro cultures, four different doses of PEG-SOD were applied to in vitro culture media during in vitro culturing following ovarian tissue vitrification and warming. To evaluate effects of PEG-SOD on ovarian tissue transplantation, four different doses of PEG-SOD were applied for 2, 7, and 21 days to mice following vitrified-warmed mouse ovarian tissue autotransplantation.

Results

The percentage of primordial follicles was maintained at the highest dose of PEG-SOD for 2 h in vitro, and there was a significant decrease in the percentage of apoptotic follicles at 2 h, but not at later time points. The highest dose of PEG-SOD also maintained primordial, primary, and secondary follicles 2 days post-transplantation, but only primordial follicles were maintained up to 21 days after transplantation.

Conclusions

PEG-SOD is protective mainly toward primordial follicles only for a short interval in vitro, presumably via antioxidant effects. PEG-SOD may be a promising additive for preserving ovarian tissue integrity, at least for primordial follicles, up to 21 days post-transplantation.

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Acknowledgement

This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI12C0055).

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea

    Eun Jung Kim, Hee Jun Lee, Jaewang Lee, Hye Won Youm, Jung Ryeol Lee & Chang Suk Suh

  2. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea

    Eun Jung Kim, Jaewang Lee, Jung Ryeol Lee, Chang Suk Suh & Seok Hyun Kim

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  1. Eun Jung Kim
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  2. Hee Jun Lee
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  3. Jaewang Lee
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  4. Hye Won Youm
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  5. Jung Ryeol Lee
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Correspondence to Jung Ryeol Lee.

Additional information

Capsule In an animal model system, PEG-SOD is shown to be a promising free-radical scavenging additive for preserving ovarian tissue integrity destined for transplantation.

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Kim, E.J., Lee, H.J., Lee, J. et al. The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation. J Assist Reprod Genet 32, 1561–1569 (2015). https://doi.org/10.1007/s10815-015-0537-8

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  • DOI: https://doi.org/10.1007/s10815-015-0537-8

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