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Development of fibrous biodegradable polymer conduits for guided nerve regeneration

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Abstract

The technique of microbraiding with modification was employed as a novel method for the fabrication of fibrous tubular scaffolds for nerve tissue engineering purposes. The biodegradable polymers used in this study were poly(L-lactide-co-glycolide) (10:90) and chitosan. The polymeric fibers were microbraided around a Teflon mandrel to make it as a tubular construct. The conduits were then studied for their surface morphology, swelling behaviour and biocompatibility. The surface morphology was analysed by scanning electron microscope, swelling behaviour by weight increase due to water uptake and biocompatibility by in vitro cytotoxicity assessment in terms of cell morphology and cell viability by the MTT assay of polymer extract treated cells. These conduits may also be used for regeneration of tissues, which require tubular scaffolds such as blood vessel, spinal cord, intestine etc.

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References

  1. CAROLE A. HEATH and GREGORY E. RUTKOWSKI, TiBtech, April 1998, Vol. 6, p. 163.

  2. CHARLES W. PATRICK JR, ANTONIOS G. MIKOS and LARRY V. MCINTIRE, “Frontiers in Tissue Engineering,” (1st edn. Pergamon, Oxford, U.K., New York, NY, U.S.A.: 1998).

    Google Scholar 

  3. MARKUS S. WIDMER, PUNEET K. GUPTA, LICHUN LU, RUDOLF K. MESZLENYI, GREGORY R. D. EVANS, KEITH BRANDT, TOM SAVEL, ALI GURLEK, CHARLES W. PATRICK JR and ANTONIOS G. MIKOS, Biomaterials 19 (1998) 1945.

    Google Scholar 

  4. ROBERT LANZA, ROBERT LANGER and WILLIAM CHICK, Principles of Tissue Engineering.

  5. J. W. FAWCETT and R. J. KEYNES, Annu. Rev. Neurosci. 13 (1990) 43.

    Google Scholar 

  6. B. R. SECKEL, Muscle and Nerve 13 (1990) 785.

    Google Scholar 

  7. M. F. MEEK, P. H. ROBINSON, I. STOKROOS, E. H. BLAAUW, G. KORS and W. F. A. DEN DUNNEN, Biomaterials 22 (2001) 1177.

    Google Scholar 

  8. M. F. MEEK, W. F. A. DEN DUNNEN, P. H. ROBINSON, A. J. PENNINGS and J. M. SCHAKENRAAD, The Intern. J. Artif. Organs 20(8) (1997) 463.

    Google Scholar 

  9. R. GIARDINO, N. NICOLI ALDINI, G. PEREGO, G. CELLA, M. C. MALTARELLO, M. FINI, M. ROCCA and G. GIAVARESI, ibid. 18 (4) (1995) 225.

    Google Scholar 

  10. M. FOIDART-DESALLE, A. DUBUISSON, A. LEJEUNE, A. SEVERYNS, Y. MANASSIS, P. DELREE, J. M. CRIELAARD, R. BASLEER and G. LEJEUNE,Experim. Neurol. 148 (1997) 236.

    Google Scholar 

  11. W. F. A. DEN DUNNEN, I. STOKROOS, E. H. BLAAUW, A. HOLWERDA, A. J. PENNINGS, P. H. ROBINSON and J. M. SCHAKENRAAD, J. Biomed. Mater. Res. 31 (1996) 105.

    Google Scholar 

  12. KYRIACOS A. ATHANASIOU, GABRIELE G. NIEDERAUER and C. MAULI AGRAWAL, Biomaterials 17 (1996) 93.

    Google Scholar 

  13. MAJETI N. V. RAVI KUMAR, React. Funct. Polym. 46 (2000) 1.

    Google Scholar 

  14. SHU WANG, ANDREW C. A. WAN, XIAOYUN XU, SHUJUN GAO, HAI-QUAN MAO, KAM W. LEONG and HANRY YU, Biomaterials 22 (2001) 1157.

    Google Scholar 

  15. N. NICOLI ALDINI, G. PEREGO, G. D. CELLA, M. C. MALTARELLO, M. FINI, M. ROCCA and R. GARDINIO, ibid. 17 (10) (1996) 959.

    Google Scholar 

  16. W. F. A. DEN DUNNEN, B. VAN DEL LEI, P. H. ROBINSON, A. HOLWERDA, A. N. PENNINGS and J. M. SCHAKENRAAD, J. Biomed. Mater. Res. 29 (1995) 757.

    Google Scholar 

  17. WEN-YUAN CHUANG, TAI-HORNG YOUNG, CHUN-HSU YAO and WEN-YEN CHIU, Biomaterials 20 (1999) 1479.

    Google Scholar 

  18. A. P. MARQUES, R. L. REIS and J. A. HUNT, ibid. 23 (2002) 1471.

    Google Scholar 

  19. A. A. IGNATIUS and L. E. CLAES, ibid. 17 (1996) 831.

    Google Scholar 

  20. ANDREW C. A. WAN, HAI-QUAN MAO, SHU WANG, KAM W. LEONG, LUCILLE K. L. L. ONG and HANRY YU, 22 (2001) 1147.

  21. Y. KOTANI, S. MATSUDA, M. SAKANAKA, K. KONDOH, S. UENO and A. SANO, J. Neurochem. 66 (5) (1996).

  22. M. F. MEEK, W. F. DEN DUNNEN, J. M. SCHAKENRAAD and P. H. ROBINSON, Microsurgery 17(10) (1996) 555.

    Google Scholar 

  23. B. R. SECKEL, D. JONES, K. J. HEKIMIAN, K. K. WANG, D. P. CHAKALIS and P. D. COSTAS, J. Neurosci. Res. 40 (1995) 318.

    Google Scholar 

  24. K. J. HEKIMIAN, B. R. SECKEL, D. J. BRYAN, K. K. WANG, D. P. CHAKALIS and A. BAILEY, J. Reconstr. Microsurg. 11(2) (1995) 93.

    Google Scholar 

  25. T. R. STEVENSON, V. A. KADHIRESAN and J. A. FAULKNER, ibid. 10(3) (1994) 171.

    Google Scholar 

  26. A. LAQUERRIERE, P. PEULVE, O. JIN, J. TIOLLIER, M. TARDY, H. VAUDRY, J. HEMET and M. TADIE, Microsurgery 15 (1994) 203.

    Google Scholar 

  27. W. F. A. DEN DUNNEN, J. M. SCHAKENRAAD, G. J. ZONDERVAN, A. J. PENNINGS, B. VAN DER LEI and P. H. ROBINSON, J. Mater. Sci.: Mater. Medic. 4 (1993) 521.

    Google Scholar 

  28. G. A. BRUNELLI, B. BATTISTON, A. VIGASIO, G. BRUNELLI and D. MAROCOLO, Microsurgery 14 (1993) 247.

    Google Scholar 

  29. S. J. ARCHIBALD, C. KRARUP, J. SHEFNER, S. T. LI and R. D. MADISON, J. Comparat. Neurol. 306 (1991) 685.

    Google Scholar 

  30. R. D. KEELEY, K. D. NGUYEN, M. J. STEPHANIDES, J. PADILLA and J. M. ROSEN, J Reconstr. Microsurg. 7(2) (1991) 93.

    Google Scholar 

  31. M. MERLE, A. L. DELLON, J. N. CAMPBELL and P. S. CHANG, Microsurgery 10 (1989) 130.

    Google Scholar 

  32. R. F. VALENTINI, P. AEBISCHER, S. R. WINN and P. M. GALLETTI, Experim. Neurol. 98 (1987) 350.

    Google Scholar 

  33. P. AEBISCHER, R. F. VALENTINI, P. DARIO, C. DOMENICI and P. M. GALETTI, Brain Res. 436 (1987) 165.

    Google Scholar 

  34. L. J. CHAMBERLAIN, I. V. YANNAS, A. ARRIZABALAGA, H. P. HSU, T. V. NORREGAARD and M. SPECTOR, Biomaterials 19 (1998) 1393.

    Google Scholar 

  35. TESSA HADLOCK, JENNIFER ELISSEEFF, ROBERT LANGER, JOSEPH VACANTI and MACK CHENEY, Arch. Otolaryng. Head Neck Surg. 124 (1998) 1081.

    Google Scholar 

  36. T. HADLOCK, C. SUNDBACK, D. HUNTER, M. CHENEY and J. P. VACANTI, Tissue Eng. 6 (2) (2000) 119.

    Google Scholar 

  37. GIOVANNI DI BENEDETTO, GERMANO ZURA, ROBERTA MAZZUCCHELLI, ALFREDO SANTINELLI, MARINA SCARPELLI and ALDO BERTANI, Biomaterials 19 (1998) 173.

    Google Scholar 

  38. GREGORY R. D. EVANS, KEITH BRANDT, ANDREAS D. NIEDERBICHER, PRISCILLA CHAUVIN, SONJA HERMANN, MELISSA BOGLE, LISA OTTA, BAO WANG and CHARLES W. PATRICK, JR., J. Biomater. Sci. Polym. Edn. 11(8) (2000) 869.

    Google Scholar 

  39. T. GILCHRIST, M. A. GLASBY, D. M. HEALY, G. KELLY, D. V. LENIHAN, K. L. MCDOWALL, I. A. MILLER and L. M. MYLES, British J. Plast. Surg. 51 (1998) 231.

    Google Scholar 

  40. R. F. VALENTINI, A. M. SABATINI, P. DARIO and P. AEBISCHER, Brain Res. 480 (1989) 300.

    Google Scholar 

  41. G. LUNDBORG, L. DAHLIN, D. DOHI, M. KANJE and N. TERADA, J. Hand Surg. 22B (3) (1997) 299.

    Google Scholar 

  42. MARIANN SONDELL, GORAN LUNDBORG and MARTIN KANJE, Brain Res. 846 (1999) 219.

    Google Scholar 

  43. SATORU YOSHII and MASANORI OKA, ibid. 888 (2001) 158.

    Google Scholar 

  44. BINI. T. B, SHUJUN GAO, XIAOYUN XU, SHU WANG, S. RAMAKRISHNA and KAM. W. LEONG, J. Biomed. Mater. Res. 68A(2) (2004) 286.

    Google Scholar 

  45. KAZUYA MATSUMOTO, KATSUNORI OHNISHI, TETSUYA KIYOTANI, TAKASHI SEKINE, HIROKI UEDA, TATSUO NAKAMURA, KATSUAKI ENDO and YASUHIKO SHIMIZU, Brain Research 868(2) (2000) 315.

    Google Scholar 

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Authors and Affiliations

  1. Bioengineering Division, Mechanical Engineering Department, National University of Singapore, Singapore, 119260

    Bini. T. B & S. Ramakrishna

  2. Molecular and Biomaterials Lab, Institute of Materials Science and Engineering, 3 Research Link, National University of Singapore, Singapore, 117602

    Shujun Gao & Shu Wang

Authors
  1. Bini. T. B
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  2. Shujun Gao
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  3. Shu Wang
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  4. S. Ramakrishna
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B, B.T., Gao, S., Wang, S. et al. Development of fibrous biodegradable polymer conduits for guided nerve regeneration. J Mater Sci: Mater Med 16, 367–375 (2005). https://doi.org/10.1007/s10856-005-0637-6

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  • DOI: https://doi.org/10.1007/s10856-005-0637-6

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