Skip to main content
SpringerLink
Log in

Antithrombogenic properties of bioconjugate streptokinase-polyglycerol dendrimers

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Dendrimers are monodisperse, spherical and hyperbranched synthetic macromolecules with a large number of surface groups that have the potential to act as carriers for drug immobilization by covalent binding or charge transfer complexation. In this work, a bioconjugate of streptokinase and a polyglycerol dendrimer (PGLD) generation 5 was used to obtain fibrinolytic surfaces. The PGLD dendrimer was synthesized by the ring opening polymerization of deprotonated glycidol using polyglycerol as core functionality in a step-growth processes denominated divergent synthesis. The PGLD dendritic structure was confirmed by gel permeation chromatography (GPC), nuclear magnetic resonance (1H-NMR, 13C-NMR) and matrix assisted laser desorption/ionization (MALDI-TOF) techniques. The synthesized dendrimer presented low dispersion in molecular weights (Mw/Mn = 1.05) and a degree of branching of 0.82 which characterize the polymer dendritic structure. The blood compatibility of the bioconjugate PGLD-Sk was evaluated by in vitro assays such as platelet adhesion and thrombus formation. Uncoated polystyrene –microtitre plates (ELISA) was used as reference. The epifluorescence microscopy results indicate that PGLD-Sk coating showed an improved antithrombogenic character relative to the uncoated ELISA plates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. A. ABRAHAM, A. A. A. DE QUEIROZ and J. SAN ROMAN, Biomaterials 23 (2002) 1625.

    Article  CAS  Google Scholar 

  2. A. A. A. DE QUEIROZ, A. GALLARDO, J. SAN ROMAN and O. Z. HIGA, J. Biomater. Sci. Polym. Edn. 7 (1995) 523.

    Google Scholar 

  3. A. A. A. DE QUEIROZ, S. C. CASTRO and O. Z. HIGA. J. Biomater. Sci. Polym. Edn. 8 (1997) 335.

    Google Scholar 

  4. A. A. A. DE QUEIROZ, E. R. BARRAK, H. A. C. GIL and O. Z. HIGA, ibid. 8 (1997) 667.

    Google Scholar 

  5. M. JOZEFOWICZ and J. JOZEFOWICZ, Biomaterials 18 (1997) 1633.

    Article  CAS  Google Scholar 

  6. T. J. SMITH and L. J. MATHIAS, Polymer 43 (2002) 7275.

    CAS  Google Scholar 

  7. D. A. TOMALIA, Angew. Chem. Int. Ed. 29 (1990) 138.

    Google Scholar 

  8. R. ESFAND and D. A. TOMALIA, Drug Discov. Today 6 (2001) 427.

    Article  CAS  Google Scholar 

  9. D. V. SAKHAROV, A. F. H. JIE, D. V. FILIPPOV, M. E. A. BEKKERS, J. H. VAN BOOM and D. C. RIJKEN, FEBS Letters 537 (2003) 6.

    Article  CAS  Google Scholar 

  10. K. BEZOUSKA, Rev. Mol. Biotechnol. 90 (2002) 269.

    CAS  Google Scholar 

  11. P. K. TRIPATHI, A. J. KHOPADE, S. NAGAICH, S. SHRIVASTAVA, S. JAIN and N. K. JAIN, Pharmazie 57 (2002) 261.

    CAS  Google Scholar 

  12. S. KASAI, H. NAGASAWA M. SHIMAMURA, Y. UTO and H. HORI, Bioorg. Med. Chem. Lett. 12 (2002) 951.

    Article  CAS  Google Scholar 

  13. S. E. FRIBERG, R. F. GOUBRAN and I. H. KAYALI, in “Food emulsions” (Marcel Dekker, New York, 1990). p.1.

    Google Scholar 

  14. D. COLLEN and H. R. LIJNEN, Fibrin.& Proteol. 14 (2000) 66.

    CAS  Google Scholar 

  15. T. T. HUANG, H. MALKE and J. J. FERRETTI, Mol. Biol. 2 (1989) 197.

    Google Scholar 

  16. F. F. BUCK and E. BOGGIANO, J. Biol. Chem. 246 (1971) 2091.

    CAS  Google Scholar 

  17. A. A. A. DE QUEIROZ, R. R. VARGAS, O. Z. HIGA, R. R. RIBEIRO and M. VITOLO, J. Appl. Polym. Sci. 84 (2002) 767.

    Google Scholar 

  18. E. C. DE RENZO, P. K. SIITERI, B. L. HUTCHING and P. H. BELL, J. Biol. Chem. 242 (1967) 533.

    Google Scholar 

  19. A. F. S. A. HABEEB, Anal. Biochem. 14 (1966) 328.

    Article  CAS  Google Scholar 

  20. F. J. MORGAN and A. HENSCHEN, Biochim. Biophys. Acta 181 (1969) 93.

    CAS  Google Scholar 

  21. T. MURAKAMI, Y. KOMIYAMA, M. MASUDA, H. KIDO, S. NOMURA, S. FUKUHARA, M. KARAKAWA, T. IWASAKA and H. TAKAHASHI, Eur. J. Clin. Inv. 26 (1996) 996.

    CAS  Google Scholar 

  22. A. A. A. DE QUEIROZ, J. Biomater. Sci. Polym. Edn. 8 (1997) 667.

    Google Scholar 

  23. W. SIESS, Physiol. Rev. 69 (1989) 58.

    CAS  Google Scholar 

  24. R. P. McEver, Thromb. Haemost. 65 (1991) 223.

    CAS  Google Scholar 

  25. T. A. SPRINGER, Cell 76 (1994) 301.

    Article  CAS  Google Scholar 

  26. S. A. HAMBURGER and R. P. McEver, Blood 75 (1990) 550.

    CAS  Google Scholar 

  27. G. Y. SHI, B. I. CHANG, S. M. CHEN, D. H. WU and H. L. WU, Biochem. J. 304 (1994) 235.

    CAS  Google Scholar 

  28. K. C. YOUNG, G. Y. Shi, Y. F. CHANG, B. I. CHANG, L. C. CHANG, M. D. LAI, W. J. CHUANG and H. L. WU, J. Biol. Chem. 270 (1995) 29601.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Física e Química, Instituto de Ciências Exatas, Universidade Federal de Itajubá (UNIFEI), Av. BPS. 1303, 37500-903, Itajubá, Minas Gerais, Brasil

    Edson Giuliani Ramos Fernandes & Alvaro Antonio Alencar De Queiroz

  2. Instituto de Investigaciones en Ciencia y Tecnología de Materiales, Intema (UNMdP-CONICET), J.B. Justo 4302, B78608FDQ, Mar del Plata, Argentina

    Gustavo A. Abraham

  3. Instituto de Ciencia y Tecnología de Polímeros, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain

    Julio San Román

Authors
  1. Edson Giuliani Ramos Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alvaro Antonio Alencar De Queiroz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gustavo A. Abraham
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Julio San Román
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Author to whom all correspondence should be addressed.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernandes, E.G.R., Queiroz, A.A.A.D., Abraham, G.A. et al. Antithrombogenic properties of bioconjugate streptokinase-polyglycerol dendrimers. J Mater Sci: Mater Med 17, 105–111 (2006). https://doi.org/10.1007/s10856-006-6813-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-6813-5

Keywords

Search

Navigation