Skip to main content

Advertisement

SpringerLink
Log in

Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Silk fibroin (SF) and alginate (AA) have been proved to be invaluable natural materials in the field of biomedical engineering. This study was designed to compare the wound healing effect of SF, AA and SF/AA-blended sponge (SF/AA) with clinically used Nu GauzeTM (CONT) in a rat full thickness wound model. Two circular skin wounds on the back of rat were covered with either of CONT, SF, AA or SF/AA. On the postoperative days of 3, 7, 10 and 14, residual wound area was calculated, and skin wound tissues were biopsied to measure the area of regenerated epithelium and collagen deposition as well as the number of proliferating cell nuclear antigen (PCNA)-immunoreactive cells. Half healing time (HT50) of SF/AA was dramatically reduced as compared with that of SF, AA or CONT. Furthermore, SF/AA significantly increased the size of re-epithelialization and the number of PCNA positive cells, whereas the effect of SF/AA on collagen deposition was not significantly different as compared with that of SF or AA. These results demonstrate that the wound healing effect of SF/AA is the best among other treatments including SF and AA, and this synergic effect is mediated by re-epithelialization via rapid proliferation of epithelial cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. E. CROSS and M. S. ROBERTS, J. Invest. Dermatol. 112 (1999) 36.

    Article  CAS  Google Scholar 

  2. M. LIU, J. D. WARN, Q. FAN and P. G. SMITH, Cell Tissue Res. 297 (1999) 423.

    Article  CAS  Google Scholar 

  3. A. SUGIHARA, K. SUGIURA, H. MORITA, T. NINAGAWA, K. TUBOUCHI, R. TUBE, M. IZUMIYA, T. HORIO, N. G. ABRAHAM and S. IKEHARA, Proc. Soc. Exp. Biol. Med. 225 (2000) 58.

    Article  CAS  Google Scholar 

  4. J. H. YEO, K. G. LEE, H. C. KIM, Y. L. OH, A. J. KIM and S. Y. KIM, Biol. Pharm. Bull. 23 (2000) 1220.

    CAS  Google Scholar 

  5. S. V. MADIBALLY, V. SOLOMON, R. N. MITCHELL, L. VAN DE WATER, M. L. YARMUSH and M. TONER, J. Surg. Res. 109 (2003) 92.

    Article  CAS  Google Scholar 

  6. P. A. HALL, D. A. LEVISON, A. L. WOODS, C. C. YU, D. B. KELLOCK, J. A. WATKINS, D. M. BARNES, C. E. GILLETT, R. CAMPLEJOHN and R. DOVER, J. Pathol. 162 (1990) 285.

    Article  CAS  Google Scholar 

  7. G. J. HERGOTT and V. I. KALNINS, Exp. Cell Res. 195 (1991) 307.

    Article  CAS  Google Scholar 

  8. J. S. KIM, V. S. MCKINNIS, K. ADAMS and S. R. WHITE, Am. J. Physiol. 273 (1997) L1235.

    CAS  Google Scholar 

  9. F. I. FRANK, F. MARINA and Y. ELAINE, Arch. Pathol. Lab. Med. 116 (1992) 1142.

    Google Scholar 

  10. N. MINOURA, S. AIBA, M. HIGUCHI, Y. GOTOH, M. TSUKADA and Y. IMAI, Biochem. Biophys. Res. Commun. 208 (1995) 511.

    Article  CAS  Google Scholar 

  11. A. THOMAS, K. G. HARDING and K. MOORE, Biomaterials 21 (2000) 1797.

    Article  CAS  Google Scholar 

  12. K. G. LEE, H. Y. KWEON, J. H. YEO, S. O. WOO, J. H. LEE and Y. H. PARK, J. Appl. Polym. Sci. 93 (2004) 2174.

    Article  CAS  Google Scholar 

  13. B. M. MIN, G. LEE, S. H. KIM, Y. S. NAM, T. S. LEE and W. H. PARK, Biomaterials 25 (2004) 1289.

    Article  CAS  Google Scholar 

  14. M. SANTIN, G. MOTTA, M. FREDDI and M. CANNAS, J. Biomed. Mater. Res. 46 (1999) 382.

    Article  CAS  Google Scholar 

  15. Y. GOTOH, S. NIIMI, T. HAYAKAWA and T. MIYASHITA, Biomaterials 25 (2004) 1131.

    Article  CAS  Google Scholar 

  16. A. B. LANSDOWN, Wound Repair Regen. 10 (2002) 271.

    Article  Google Scholar 

  17. S. D. BLAIR, C. M. BACKHOUSE, R. HARPER, J. MATTHEWS and C. N. MCCOLLUM, Br. J. Surg. 75 (1988) 969.

    CAS  Google Scholar 

  18. J. M. O’DONOGHUE, S. T. O’SULLIVAN, E. S. BEAUSANG, J. I. PANCHAL, M. O’SHAUGHNESSY and T. P. O’CONNOR, Acta. Chir. Plast. 39 (1997) 53.

    CAS  Google Scholar 

  19. Y. S. CHOI, S. R. HONG, Y. M. LEE, K. W. SONG, M. H. PARK and Y. S. NAM, Biomaterials 20 (1999) 409.

    Article  CAS  Google Scholar 

  20. A. L. ATTWOOD, Br. J. Plast. Surg. 42 (1989) 373.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, South Korea

    Dae-Hyun Roh, Seuk-Yun Kang, Jeom-Yong Kim & Jang-Hern Lee

  2. Department of Pharmacology, Institute for Medical Science, Chonbuk National University Medical School, Chonju, 561-180, South Korea

    Young-Bae Kwon

  3. Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon, 441-100, South Korea

    Hae Young Kweon & Kwang-Gill Lee

  4. School of Biological Resources and Materials Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, South Korea

    Young-Hwan Park

  5. Department of Plastic Surgery, Seoul National University Bundang Hospital, Seongnam, 463-707, South Korea

    Rong-Min Baek & Chan-Yeong Heo

  6. Department of Plastic Surgery, Inje University Sanggye-Paik Hospital, Seoul, 139-707, South Korea

    Joon Choe

Authors
  1. Dae-Hyun Roh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seuk-Yun Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeom-Yong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Young-Bae Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hae Young Kweon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kwang-Gill Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Young-Hwan Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Rong-Min Baek
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Chan-Yeong Heo
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Joon Choe
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Jang-Hern Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jang-Hern Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roh, DH., Kang, SY., Kim, JY. et al. Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat. J Mater Sci: Mater Med 17, 547–552 (2006). https://doi.org/10.1007/s10856-006-8938-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-8938-y

Keywords

Search

Navigation