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Fabrication and characterization of chitosan microcarrier for hepatocyte culture

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Abstract

Using chitosan as raw materials, a suitable size (300–500 μm) of porous microcarrier was fabricated by suspension crosslinking and lyophilizing method, which made the carrier has an average pore size of 50 μm and 86% porosity. The microcarrier was modified with lactose and maltose respectively. Various factors that influenced the preparation of microcarrier were studied and the reaction conditions were optimized. Rat hepatocytes cultured on modified microcarrier retained a spherical shape which is similar to those in vivo and formed aggregates. The metabolic activities of cells on lactose-modified were higher than those on maltose-modified microcarrier. The highest albumin secretion reached 54.8 μg/106 cells/d, and the highest urea synthesis reached 4.65 μmol/106cells/d, which may be promoted by the formation of cellular aggregates. In conclusion, lactose-modified porous microcarrier is promising scaffold for hepatocytes culture.

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Acknowledgments

The national Natural Science Foundation of China and the Natural Science Foundation of Tianjin are well acknowledged by the authors for the financial support.

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Authors and Affiliations

  1. The Key Laboratory of Bioactive Materials, Nankai University, Ministry of Education, Tianjin, 300071, China

    Chen Wu, Jilun Pan, Zhiming Bao & Yaoting Yu

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  1. Chen Wu
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  2. Jilun Pan
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  3. Zhiming Bao
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  4. Yaoting Yu
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Correspondence to Jilun Pan.

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Wu, C., Pan, J., Bao, Z. et al. Fabrication and characterization of chitosan microcarrier for hepatocyte culture. J Mater Sci: Mater Med 18, 2211–2214 (2007). https://doi.org/10.1007/s10856-007-3071-0

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  • DOI: https://doi.org/10.1007/s10856-007-3071-0

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