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Coating of polyurethane scaffolds with collagen: comparison of coating and cross-linking techniques

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Abstract

Collagen has been coated successfully onto numerous hydrophilic polymer scaffolds to improve cell adhesion. Due to the hydrophobic nature of thermoplastic polyurethane (TPU), coating with aqueous collagen solution is problematic for such scaffolds. This study facilitated the coating of TPU with collagen and compared cross-linking and coating techniques. Three different cross-linking methods were compared. Both thermal and glutaraldehyde methods showed proof of cross-linking; however glutaraldehyde seemed to be superior to the other methods. The use of human urine as a wetting agent and the chemical glutaraldehyde had no effect on a cytotoxicity test performed by means of a WST-1 assay with a fibroblastic cell line. Three different coating techniques for porous TPU scaffolds were also investigated: ultrasound, pressurized air and injection. Of these, injection performed best. This method facilitated a coating of 100% of the porous scaffolds examined, which was verified by staining, FTIR and SEM.

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References

  1. H. Chevallay, Cell. Eng. Tissue Eng. Biomater. 38, 211 (2000)

    CAS  Google Scholar 

  2. B.S. Park, S.J. Heo, C.S. Kim, J.E. Oh, J.M. Kim, G. Lee, W.H. Park, C.P. Chung, B.M. Min, J. Biomed. Mater. Res. A 74, 640 (2005)

    Google Scholar 

  3. H.W. Kim, L.H. Li, E.J. Lee, S.H. Lee, H.E. Kim, J. Biomed. Mater. Res. A 75, 629 (2005)

    Google Scholar 

  4. U. Geissler, U. Hempel, C. Wolf, D. Scharnweber, H. Worch, K. Wenzel, J. Biomed. Mater. Res. 51, 752 (2000)

    Article  CAS  Google Scholar 

  5. C. Roehlecke, M. Witt, M. Kasper, E. Schulze, C. Wolf, A. Hofer, R.W. Funk, Cells Tissues Organs 168, 178 (2001)

    Article  CAS  Google Scholar 

  6. K. Gelse, E. Poschl, T. Aigner, Adv. Drug Deliv. Rev. 55, 1531 (2003)

    Article  CAS  Google Scholar 

  7. J. Myllyharju, K.I. Kivirikko, Trends Genet. 20, 33 (2004)

    Article  CAS  Google Scholar 

  8. M.G. Patino, M.E. Neiders, S. Andreana, B. Noble, R.E. Cohen, Implant Dent. 11, 280 (2002)

    Article  Google Scholar 

  9. D.J. Aframian, E. Cukierman, J. Nikolovski, D.J. Mooney, K.M. Yamada, B.J. Baum, Tissue Eng. 6, 209 (2000)

    Article  CAS  Google Scholar 

  10. H.C. Fiegel, J. Havers, U. Kneser, M.K. Smith, T. Moeller, D. Kluth, D.J. Mooney, X. Rogiers, P.M. Kaufmann, Tissue Eng. 10, 165 (2004)

    Article  CAS  Google Scholar 

  11. S.H. Hsu, C.Y. Chen, P.S. Lu, C.S. Lai, C.J. Chen, Biotechnol. Bioeng. 92, 579 (2005)

    Article  CAS  Google Scholar 

  12. G. Liu, Y.Y. Hu, Y.N. Yan, Z. Xiong, Z. Wang, R. Lu, J.P. Bai, J.J. Yang, Zhonghua Yi Xue Za Zhi 83, 580 (2003)

    CAS  Google Scholar 

  13. T. Qin, Z. Yang, H. Xie, H. Li, J. Qin, Z. Wu, S. Xu, S. Cai, Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 19, 633 (2002)

    CAS  Google Scholar 

  14. Y.C. Wu, S.Y. Shaw, H.R. Lin, T.M. Lee, C.Y. Yang, Biomaterials 27, 896 (2006)

    Article  CAS  Google Scholar 

  15. M.D. Phaneuf, D.J. Dempsey, M.J. Bide, W.C. Quist, F.W. LoGerfo, Biomaterials 22, 463 (2001)

    Article  CAS  Google Scholar 

  16. L. Mei, X. Jin, C. Song, M. Wang, R.J. Levy, J. Gene Med. 8, 690 (2006)

    Article  CAS  Google Scholar 

  17. B. Krijgsman, A.M. Seifalian, H.J. Salacinski, N.R. Tai, G. Punshon, B.J. Fuller, G. Hamilton, Tissue Eng. 8, 673 (2002)

    Article  CAS  Google Scholar 

  18. J. Gerlach, H.H. Schauwecker, E. Hennig, E.S. Bucherl, Artif Organs 13, 144 (1989)

    Article  CAS  Google Scholar 

  19. S. De, R. Sharma, S. Trigwell, B. Laska, N. Ali, M.K. Mazumder, J.L. Mehta, J. Biomater. Sci. Polym. Ed. 16, 973 (2005)

    Article  CAS  Google Scholar 

  20. F.J. Clubb Jr., D.L. Clapper, D.A. Deferrari, S.P. Hu, W.J. Seare Jr., P.P. Capek, J. Armstrong, M.G. McGee, L.A. Billings, J.M. Fuqua Jr., S.M. Parins, ASAIO J. 45, 281 (1999)

    Article  CAS  Google Scholar 

  21. E.H. Cho, Y.I. Yang, C.W. Mun, J.K. Kim, J. Biomater. Sci. Polym. Ed. 16, 775 (2005)

    Article  CAS  Google Scholar 

  22. T. Bonner, D.S. Jones, S.P. Gorman, Int. J. Pharm. 151, 201 (1997)

    Article  CAS  Google Scholar 

  23. J.G. McGovern, C.P. Garvin, D.S. Jones, A.D. Woolfson, S.P. Gorman, Int. J. Pharm. 149, 251 (1997)

    Article  CAS  Google Scholar 

  24. M. Santin, A. Motta, S.P. Denyer, M. Cannas, Biomaterials 20, 1245 (1999)

    Article  CAS  Google Scholar 

  25. M.J. van Luyn, P.B. van Wachem, L.H. Olde Damink, P.J. Dijkstra, J. Feijen, P. Nieuwenhuis, Biomaterials 13, 1017 (1992)

    Article  Google Scholar 

  26. V. Charulatha, A. Rajaram, Biomaterials 24, 759 (2003)

    Article  CAS  Google Scholar 

  27. C.L. Tsai, S.H. Hsu Sh, W.L. Cheng, Artif. Organs 26, 18 (2002)

    Article  CAS  Google Scholar 

  28. J.M. Lee, H.H.L. Edwards, C.A. Pereira, S.I. Samii, J. Mater. Sci. Mater. Med. 7, 531 (1996)

    Article  CAS  Google Scholar 

  29. L. Ma, C.Y. Gao, Z.W. Mao, J. Zhou, J.C. Shen, J. Biomed. Mater. Res. A 71A, 334 (2004)

    Article  CAS  Google Scholar 

  30. H. Haugen, W. Fuchs, M. Brunner, J. Will, J. Aigner, E. Wintermantel, J. Biomed. Mater. Res. B 77B, 65 (2005)

    Google Scholar 

  31. H. Haugen, V. Ried, M. Brunner, J. Will, E. Wintermantel, J. Mater. Sci. Mater. Med. 15, 343 (2004)

    Article  CAS  Google Scholar 

  32. W.M.L. Neethling, A.J. Hodge, R. Glancy, J. Biomed. Mater. Res. B Appl. Biomater. 66B, 356 (2003)

    Article  CAS  Google Scholar 

  33. M. Hendriks, M. Verhoeven, P. Cahalan, R. Zeeman, P. Dijkstra, J. Feijen, US, 2000

  34. Noshiki, Artif. Organs 22, 672 (1998)

  35. M.I. Benigno, R.A. Azeredo, J.L. Lemos, B. Konig Jr., E.A. Liberti, J. Oral Rehabil. 28, 113 (2001)

    Article  CAS  Google Scholar 

  36. A.M. Clement, K. Sugahara, A. Faissner, Neurosci. Lett. 269, 125 (1999)

    Article  CAS  Google Scholar 

  37. A.T.C.C. American Type Culture Collection, Catalogue of Cell Lines and Hybridomas (ATCC, Manassass, 1992)

  38. W. Lamba, Cooper, Polyurethanes in Biomedical Applications (CHC, Boca Raton, 1998)

  39. J.M. Chen, R.T. Aimes, G.R. Ward, G.L. Youngleib, J.P. Quigley, J. Biol. Chem. 266, 5113 (1991)

    CAS  Google Scholar 

  40. M.C. Bonner, M.M. Tunney, D.S. Jones, S.P. Gorman, Int. J. Pharm. 151, 201 (1997)

    Article  CAS  Google Scholar 

  41. Meade, Bioorg. Med. Chem. 11 (2003)

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Authors and Affiliations

  1. Institute of Material Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Strasse 27, 01069, Dresden, Germany

    Timothy Douglas

  2. Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, P.O Box 1109, 0317, Oslo, Norway

    Håvard J. Haugen

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  1. Timothy Douglas
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  2. Håvard J. Haugen
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Correspondence to Timothy Douglas.

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Douglas, T., Haugen, H.J. Coating of polyurethane scaffolds with collagen: comparison of coating and cross-linking techniques. J Mater Sci: Mater Med 19, 2713–2719 (2008). https://doi.org/10.1007/s10856-008-3393-6

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  • DOI: https://doi.org/10.1007/s10856-008-3393-6

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