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Immobilization of poly (ethylene imine) on poly (l-lactide) promotes MG63 cell proliferation and function

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Abstract

Poly (ethylene imine) (PEI) is a polycation widely used for DNA transfection to cells but also applied as primary polycation for layer-by-layer (LBL) assembly of polyelectrolytes. The aim of the present study was to investigate the effect of modification with PEI on the biocompatibility of poly (l-lactide) (PLLA) films. PEI with different molecular weight was immobilized on PLLA by either adsorption or covalent binding. Cell morphologies, immuno-fluorescence staining, cell proliferation by lactate dehydrogenase assay and cell differentiation by alkaline phosphatase assay were utilized to assess the biocompatibility of the modified PLLA using osteoblast cell line MG63. Results revealed that PEI modification remarkably improved cell adhesion, viability, proliferation and function compared with plain PLLA. Hence, PEI-modified PLLA is acceptable as transfection vehicle for engineering of bone and other tissues, or as primary layer to allow LBL assembly to generate biomimetic surface coatings.

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Acknowledgments

This work was supported by the Marie-Curie Incoming International Fellowship (Contract No. MIF1-CT-2005-021854) of European Commission to Dr. Z.-M. Liu. The authors are grateful to Prof. Shui-Ju Wang from State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, P. R. China for XPS measurements.

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Authors and Affiliations

  1. Institute of Polymer Science, Zhejiang University, Hangzhou, 310027, People’s Republic of China

    Zhen-Mei Liu

  2. Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Biomedical Materials Group, Martin-Luther-University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120, Halle (Saale), Germany

    Soo-Yeon Lee, Sukhéna Sarun, Dieter Peschel & Thomas Groth

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  1. Zhen-Mei Liu
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  2. Soo-Yeon Lee
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Correspondence to Zhen-Mei Liu or Thomas Groth.

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Liu, ZM., Lee, SY., Sarun, S. et al. Immobilization of poly (ethylene imine) on poly (l-lactide) promotes MG63 cell proliferation and function. J Mater Sci: Mater Med 20, 2317–2326 (2009). https://doi.org/10.1007/s10856-009-3806-1

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