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Enhanced cell adhesion to silicone implant material through plasma surface modification

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Abstract

Silicone implant material is widely used in the field of plastic surgery. Despite its benefits the lack of biocompatibility this material still represents a major problem. Due to the surface characteristics of silicone, protein adsorption and cell adhesion on this polymeric material is rather low. The aim of this study was to create a stable collagen I surface coating on silicone implants via glow-discharge plasma treatment in order to enhance cell affinity and biocompatibility of the material. Non-plasma treated, collagen coated and conventional silicone samples (non-plasma treated, non-coated) served as controls. After plasma treatment the change of surface free energy was evaluated by drop-shape analysis. The quality of the collagen coating was analysed by electron microscopy and Time-Of-Flight Secondary Ion Mass Spectrometry. For biocompatibility tests mouse fibroblasts 3T3 were cultivated on the different silicone surfaces and stained with calcein-AM and propidium iodine to evaluate cell viability and adherence. Analysis of the different surfaces revealed a significant increase in surface free energy after plasma pre-treatment. As a consequence, collagen coating could only be achieved on the plasma activated silicone samples. The in vitro tests showed that the collagen coating led to a significant increase in cell adhesion and cell viability.

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Authors and Affiliations

  1. Department of Plastic Surgery, BG Trauma Center Bergmannsheil, University Hospital, 44789, Bochum, Germany

    J. Hauser & H. U. Steinau

  2. Department of Surgical Research, BG Trauma Center Bergmannsheil, University Hospital, 44789, Bochum, Germany

    J. Zietlow & M. Köller

  3. Institute for Plasma Technology, Ruhr-University, 44801, Bochum, Germany

    H. Halfmann & P. Awakowicz

  4. Department of Surgery, BG Trauma Center Bergmannsheil, University Hospital, 44789, Bochum, Germany

    S. A. Esenwein

  5. Department of Plastic Surgery, BG-Kliniken Bergmannsheil, University Hospital, Buerkle-de-la-Camp-Platz 1, 44789, Bochum, Germany

    J. Hauser

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  1. J. Hauser
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  4. S. A. Esenwein
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  7. H. U. Steinau
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Correspondence to J. Hauser.

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Hauser, J., Zietlow, J., Köller, M. et al. Enhanced cell adhesion to silicone implant material through plasma surface modification. J Mater Sci: Mater Med 20, 2541–2548 (2009). https://doi.org/10.1007/s10856-009-3826-x

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  • DOI: https://doi.org/10.1007/s10856-009-3826-x

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