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Fabrication and biocompatibility of nano non-stoichiometric apatite and poly(ε-caprolactone) composite scaffold by using prototyping controlled process

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Abstract

Nano biocomposite scaffolds of non-stoichiometric apatite (ns-AP) and poly(ε-caprolactone) (PCL) were prepared by a prototyping controlled process (PCP). The results show that the composite scaffolds with 40 wt% ns-AP contained open and well interconnected pores with a size of 400–500 μm, and exhibited a maximum porosity of 76%. The ns-AP particles were not completely embedded in PCL matrix while exposed on the composite surface, which might be useful for cell attachment and growth. Proliferation of MG63 cells was significantly better on the composite scaffolds with porosity of 76% than that those with porosity of 53%, indicating that the scaffolds with high porosity facilitated cell growth, and could promote cell proliferation. The composite scaffolds were implanted into rabbit thighbone defects to investigate the in vivo biocompatibility and osteogenesis. Radiological and histological examination confirmed that the new bony tissue had grown easily into the entire composite scaffold. The results suggest that the well-interconnected pores in the scaffolds might encourage cell proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the scaffolds. This study shows that bioactive and biocompatible ns-AP/PCL composite scaffolds have potential applications in bone tissue engineering.

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Acknowledgements

The authors would like to thank Ms. Fan Minghui from Institute of Chemistry and Materials Science, University of Science and Technology of China for her assistance in material preparation of this paper.

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Authors and Affiliations

  1. Guanghua School of Stomatology, Sun Yat-sen University, 510080, Guangzhou, People’s Republic of China

    Liang Ye

  2. Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People’s Republic of China

    Xinchen Zeng & Haojiang Li

  3. College of Life Sciences, Sun Yat-sen University, 510080, Guangzhou, People’s Republic of China

    Yi Ai

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  1. Liang Ye
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Correspondence to Xinchen Zeng or Haojiang Li.

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Ye, L., Zeng, X., Li, H. et al. Fabrication and biocompatibility of nano non-stoichiometric apatite and poly(ε-caprolactone) composite scaffold by using prototyping controlled process. J Mater Sci: Mater Med 21, 753–760 (2010). https://doi.org/10.1007/s10856-009-3872-4

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  • DOI: https://doi.org/10.1007/s10856-009-3872-4

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