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Evaluating cell proliferation based on internal pore size and 3D scaffold architecture fabricated using solid freeform fabrication technology

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Abstract

The scaffold, as a medical component to regenerate tissues or organs in humans, plays an important role in tissue engineering. Recently, solid freeform fabrication (SFF) technology using computer-assisted methods was applied to address the problems of conventional fabrication methods in which the internal/outer architectures cannot be controlled. In this report, we propose suitable scaffolds for bone tissue regeneration considering the internal pore size and scaffold architecture. Poly(propylene fumarate) was used as the biodegradable photopolymer, and scaffolds were fabricated using microstereolithography (MSTL). We observed the relationship between the internal pores and architecture, and the proliferation of pre-osteoblast cells. To demonstrate the superiority of MSTL, we fabricated conventional and SFF scaffolds, and measured the cell proliferation rates for each. The results showed that cell proliferation on the MSTL scaffold was clearly superior and indicated that MSTL would be a good replacement for current conventional methods.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0018294 & NRF-2009-352-D00024) and WCU (World Class University) program through the Korea Science and Engineering Foundation funded by the Ministry of Education, Science and Technology (R31-2008-000-10105-0).

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Authors and Affiliations

  1. Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX, 78712-0292, USA

    Jin Woo Lee

  2. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Gyungbuk, 790-781, South Korea

    Geunseon Ahn & Dong-Woo Cho

  3. Department of Mechanical Engineering, Andong National University, 388, Songchun-dong, Andong-si, Gyungbuk, 760-749, South Korea

    Jong Young Kim

  4. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Gyungbuk, 790-781, South Korea

    Dong-Woo Cho

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  1. Jin Woo Lee
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  2. Geunseon Ahn
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Correspondence to Dong-Woo Cho.

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Lee, J.W., Ahn, G., Kim, J.Y. et al. Evaluating cell proliferation based on internal pore size and 3D scaffold architecture fabricated using solid freeform fabrication technology. J Mater Sci: Mater Med 21, 3195–3205 (2010). https://doi.org/10.1007/s10856-010-4173-7

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  • DOI: https://doi.org/10.1007/s10856-010-4173-7

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