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Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

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Abstract

Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by self-assembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS–ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P < 0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC, 81071274), International S & T Cooperation Program of China (ISTCP, S2011GR0092).

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, 5# Yushan Road, 266003, Qingdao, People’s Republic of China

    Ya Liu, Xiao Jie Cheng, Qi Feng Dang, Fang Kui Ma & Xi Guang Chen

  2. College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Hyun Jin Park

  3. Korea Food Research Institute, Seoul, 463-746, Korea

    Bum Keun Kim

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  1. Ya Liu
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Correspondence to Xi Guang Chen.

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Liu, Y., Cheng, X.J., Dang, Q.F. et al. Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers. J Mater Sci: Mater Med 23, 375–384 (2012). https://doi.org/10.1007/s10856-011-4470-9

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