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Preparation and biocompatibility study of in situ forming polymer implants in rat brains

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Abstract

We describe the development of polymer implants that were designed to solidify once injected into rat brains. These implants comprised of glycofurol and copolymers of d,l-lactide (LA), ε-caprolactone and poly(ethylene glycol) (PLECs). Scanning electron microscopy (SEM) and gel permeation chromatography (GPC) showed that the extent of implant degradation was increased with LA content in copolymers. SEM analysis revealed the formation of porosity on implant surface as the degradation proceeds. PLEC with 19.3% mole of LA was chosen to inject in rat brains at the volume of 10, 25 and 40 μl. Body weights, hematological and histopathological data of rats treated with implants were evaluated on day 3, 6, 14, 30 and 45 after the injection. Polymer solution at the injection volume of 10 μl were tolerated relatively well compared to those of 25 and 40 μl as confirmed by higher body weight and healing action (fibrosis tissue) 30 days after treatment. The results from this study suggest a possible application as drug delivery systems that can bypass the blood brain barrier.

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Acknowledgments

This research project is supported by Mahidol University, Thailand. We thank the National Institute of Health, Department of Medical Sciences, Ministry of Public Health for the help in animal care and handling.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, 25/25 Puttamonthon 4th Rd., Nakorn Pathom, 73170, Thailand

    Norased Nasongkla & Chawan Manaspon

  2. Department of Surgery, Neurosurgical Unit, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand

    Atthaporn Boongird

  3. Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand

    Suradej Hongeng

  4. Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand

    Noppadol Larbcharoensub

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  1. Norased Nasongkla
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  2. Atthaporn Boongird
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Correspondence to Norased Nasongkla.

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Nasongkla, N., Boongird, A., Hongeng, S. et al. Preparation and biocompatibility study of in situ forming polymer implants in rat brains. J Mater Sci: Mater Med 23, 497–505 (2012). https://doi.org/10.1007/s10856-011-4520-3

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  • DOI: https://doi.org/10.1007/s10856-011-4520-3

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