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Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing

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Abstract

Electrospun gelatin and poly-ε-caprolactone (PCL) nanofibers were prepared using needleless technology and their biocompatibility and therapeutic efficacy have been characterized in vitro in cell cultures and in an experimental model of a skin wound. Human dermal fibroblasts, keratinocytes and mesenchymal stem cells seeded on the nanofibers revealed that both nanofibers promoted cell adhesion and proliferation. The effect of nanofibers on wound healing was examined using a full thickness wound model in rats and compared with a standard control treatment with gauze. Significantly faster wound closure was found with gelatin after 5 and 10 days of treatment, but no enhancement with PCL nanofibers was observed. Histological analysis revealed enhanced epithelialisation, increased depth of granulation tissue and increased density of myofibroblasts in the wound area with gelatin nanofibers. The results show that gelatin nanofibers produced by needleless technology accelerate wound healing and may be suitable as a scaffold for cell transfer and skin regeneration.

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Acknowledgments

The financial support of the Grant Agency of the Czech Republic (No. 304/07/1129), the Grant Agency of the Academy of Science of the Czech Republic (KAN200520804 and IAA500390902), the Ministry of Education, Youth and Sports of the Czech Republic (2B06130, 1M0538, 1M0506) and the Ministry of Health of the Czech Republic (MZO00023001) is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Michal Dubský, Šárka Kubinová, Petr Lesný & Eva Syková

  2. Institute for Clinical and Experimental Medicine, Videnska 1958/9, 14021, Prague 4, Czech Republic

    Michal Dubský, Luděk Voska & Alexandra Jirkovská

  3. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Jakub Širc & Jiří Michálek

  4. Elmarco Ltd, Liberec, Czech Republic

    Marcela Munzarová

  5. Prague Burn Center, Third Faculty of Medicine and Teaching Hospital Kralovske Vinohrady, Charles University, Prague, Czech Republic

    Robert Zajíček

  6. Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Alena Zajícová & Vladimír Holáň

  7. Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic

    Jiří Michálek & Eva Syková

Authors
  1. Michal Dubský
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  2. Šárka Kubinová
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Correspondence to Michal Dubský.

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Dubský, M., Kubinová, Š., Širc, J. et al. Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing. J Mater Sci: Mater Med 23, 931–941 (2012). https://doi.org/10.1007/s10856-012-4577-7

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  • DOI: https://doi.org/10.1007/s10856-012-4577-7

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