Skip to main content
SpringerLink
Log in

Silver-containing mesoporous bioactive glass with improved antibacterial properties

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

The aim of the present work is the study of the bacteriostatic/bactericidal effect of a silver-containing mesoporous bioactive glass obtained by evaporation-induced self-assembly and successive thermal stabilization. Samples of the manufactured mesophase were characterized by means of transmission electron microscopy and N2 adsorption/desorption at 77 K, revealing structural and textural properties similar to SBA-15 mesoporous silica. Glass samples used for bioactivity experiments were put in contact with a standardized, commercially available cell culture medium instead of lab-produced simulated body fluid, and were then characterized by means of X-ray diffraction, field emission scanning electron microscopy and Fourier transform infrared spectroscopy. All these analyses confirmed the development of a hydroxyl carbonate apatite layer on glass particles. Moreover, the investigated mesostructure showed a very good antibacterial effect against S. aureus strain, with a strong evidence of bactericidal activity already registered at 0.5 mg/mL of glass concentration. A hypothesis about the mechanism by which Ag affects the bacterial viability, based on the intermediate formation of crystalline AgCl, was also taken into account. With respect to what already reported in the literature, these findings claim a deeper insight into the possible use of silver-containing bioactive glasses as multifunctional ceramic coatings for orthopedic devices.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Ciesla U, Schüth F. Ordered mesoporous materials. Microporous Mesoporous Mater. 1999;27:131–49.

    Article  CAS  Google Scholar 

  2. Santos HA, Salonen J, Bimbo LM, Lehto VP, Peltonen L, Hirvonen J. Mesoporous materials as controlled drug delivery formulations. J Drug Deliv Sci Technol. 2011;21:139–55.

    CAS  Google Scholar 

  3. Gargiulo N, De Santo I, Causa F, Caputo D, Netti PA. Confined mesoporous silica membranes for albumin zero-order release. Microporous Mesoporous Mater. 2013;167:71–5.

    Article  CAS  Google Scholar 

  4. Vallet-Regí M, Ruiz-González L, Izquierdo-Barba I, González-Calbet JM. Revisiting silica based ordered mesoporous materials: medical applications. J Mater Chem. 2006;16:26–31.

    Article  Google Scholar 

  5. Yan XX, Deng HX, Huang XH, Lu GQ, Qiao SZ, Zhao DY, Yu CZ. Mesoporous bioactive glasses. I. Synthesis and structural characterization. J Non Cryst Solids. 2005;351:3209–17.

    Article  CAS  Google Scholar 

  6. Brinker CJ, Lu Y, Sellinger A, Fan H. Evaporation-induced self-assembly: nanostructures made easy. Adv Mater. 1999;11:579–85.

    Article  CAS  Google Scholar 

  7. Yan X, Huang X, Yu C, Deng H, Wang Y, Zhang Z, Qiao S, Lu G, Zhao D. The in vitro bioactivity of mesoporous bioactive glasses. Biomaterials. 2006;27:3396–403.

    Article  CAS  Google Scholar 

  8. Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res. 1990;24:721–34.

    Article  CAS  Google Scholar 

  9. Cabal B, Malpartida F, Torrecillas R, Hoppe A, Boccaccini AR, Moya JS. The development of bioactive glass-ceramic substrates with biocide activity. Adv Eng Mater. 2011;13:B462–6.

    Article  Google Scholar 

  10. El-Kady AM, Ali AF, Rizk RA, Ahmed MM. Synthesis, characterization and microbiological response of silver doped bioactive glass nanoparticles. Ceram Int. 2012;38:177–88.

    Article  CAS  Google Scholar 

  11. Nezafati N, Moztarzadeh F, Hesaraki S, Mozafari M, Samadikuchaksaraei A, Hajibaki L, Gholipour M. Effect of silver concentration on bioactivity and antibacterial properties of SiO2–CaO–P2O5 sol–gel derived bioactive glass. Key Eng Mater. 2012;493–494:74–9.

    Google Scholar 

  12. Zimmerli W, Sendi P. Pathogenesis of implant-associated infection: the role of the host. Semin Immunopathol. 2011;33:295–306.

    Article  CAS  Google Scholar 

  13. Campoccia D, Montanaro L, Speziale P, Arciola CR. Antibiotic-loaded biomaterials and the risks for the spread of antibiotic resistance following their prophylactic and therapeutic clinical use. Biomaterials. 2010;31:6363–77.

    Article  CAS  Google Scholar 

  14. Knetsch MLW, Koole LH. New strategies in the development of antimicrobial coatings: the example of increasing usage of silver and silver nanoparticles. Polymers. 2011;3:340–66.

    Article  CAS  Google Scholar 

  15. Hu G, Xiao L, Tong P, Bi D, Wang H, Ma H, Zhu G, Liu H. Antibacterial hemostatic dressings with nanoporous bioglass containing silver. Int J Nanomed. 2012;7:2613–20.

    Article  CAS  Google Scholar 

  16. Wang X, Li X, Onuma K, Ito A, Sogo Y, Kosuge K, Oyane A. Mesoporous bioactive glass coatings on stainless steel for enhanced cell activity, cytoskeletal organization and AsMg immobilization. J Mater Chem. 2010;20:6437–45.

    Article  CAS  Google Scholar 

  17. Bohner M, Lemaitre J. Can bioactivity be tested in vitro with SBF solution? Biomaterials. 2009;30:2175–9.

    Article  CAS  Google Scholar 

  18. Mandel S, Tas AC. Brushite (CaHPO4···2H2O) to octacalcium phosphate (Ca8(HPO4)2(PO4)4···5H2O) transformation in DMEM solutions at 36.5 °C. Mater Sci Eng C. 2010;30:245–54.

    Article  CAS  Google Scholar 

  19. Lee JTY, Leng Y, Chow KL, Ren F, Ge X, Wang K, Lu X. Cell culture medium as an alternative to conventional simulated body fluid. Acta Biomater. 2011;7:2615–22.

    Article  CAS  Google Scholar 

  20. Zhao D, Huo Q, Feng J, Chmelka BF, Stucky GD. Nonionic triblock and star diblock copolymer and oligomeric surfactant syntheses of highly ordered, hydrothermally stable, mesoporous silica structures. J Am Chem Soc. 1998;120:6024–36.

    Article  CAS  Google Scholar 

  21. Lowell S, Shields JE, Thomas MA, Thommes M. Characterization of porous solids and powders: surface area, pore size and density. 1st ed. Dordrecht: Kluwer Academic Publishers; 2004.

    Book  Google Scholar 

  22. Bellantone M, Coleman NJ, Hench LL. Bacteriostatic action of a novel four-component bioactive glass. J Biomed Mater Res. 2000;51:484–90.

    Article  CAS  Google Scholar 

  23. Wei G, Yan X, Yi J, Zhao L, Zhou L, Wang Y, Yu C. Synthesis and in vitro bioactivity of mesoporous bioactive glasses with tunable macropores. Microporous Mesoporous Mater. 2011;143:157–65.

    Article  CAS  Google Scholar 

  24. López-Noriega A, Arcos D, Vallet-Regí M. Functionalizing mesoporous bioglasses for long-term anti-osteoporotic drug delivery. Chem Eur J. 2010;16:10879–86.

    Article  Google Scholar 

  25. López-Noriega A, Arcos D, Izquierdo-Barba I, Sakamoto Y, Terasaki O, Vallet-Regí M. Ordered mesoporous bioactive glasses for bone tissue regeneration. Chem Mater. 2006;18:3137–44.

    Article  Google Scholar 

  26. Bellantone M, Williams HD, Hench LL. Broad-spectrum bactericidal activity of Ag2O-doped bioactive glass. Antimicrob Agent Chemother. 2002;46:1940–5.

    Article  CAS  Google Scholar 

  27. Tuncer M, Seker E. Single step sol–gel made silver chloride on titania xerogels to inhibit E. coli bacteria growth: effect of preparation and chloride ion on bactericidal activity. J Sol–Gel Sci Technol. 2011;59:304–10.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the help provided by Dr. Valentina Mollo (Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia) and Dr. Manlio Colella (Centro di Ricerca Interdipartimentale sui Biomateriali, Università Federico II) for the help provided in performing TEM investigations.

Author information

Authors and Affiliations

  1. Centro di Ricerca Interdipartimentale sui Biomateriali, Università Federico II, P.le Tecchio 80, 80125, Naples, Italy

    Nicola Gargiulo, Filippo Causa & Paolo Antonio Netti

  2. Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Naples, Italy

    Angela Maria Cusano, Filippo Causa & Paolo Antonio Netti

  3. Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università Federico II, P.le Tecchio 80, 80125, Naples, Italy

    Filippo Causa, Domenico Caputo & Paolo Antonio Netti

Authors
  1. Nicola Gargiulo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angela Maria Cusano
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Filippo Causa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Domenico Caputo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paolo Antonio Netti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Filippo Causa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gargiulo, N., Cusano, A.M., Causa, F. et al. Silver-containing mesoporous bioactive glass with improved antibacterial properties. J Mater Sci: Mater Med 24, 2129–2135 (2013). https://doi.org/10.1007/s10856-013-4968-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-013-4968-4

Keywords

Search

Navigation