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Infiltration and sustenance of viability of cells by amphiphilic biosynthetic biodegradable hydrogels

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Abstract

Amphiphilic biosynthetic hydrogels comprising natural polysaccharide alginate (I) and synthetic polyester polypropylene fumarate (II) units were prepared by crosslinking the copolymer of I and II with calcium ion and vinyl monomers viz, 2-hydroxyethyl methacrylate (HEMA), methyl methacrylate (MMA), butyl methacrylate (BMA) and N,N′-methylene bisacrylamide (NMBA). Three fast degradable hydrogels, ALPF-MMA, ALPF-HEMA and ALPF-BMA and one slow degradable hydrogel ALPF-NMBA were prepared. These hydrogels are amphiphilic and able to hold sufficient amount of proteins on their surfaces. All these hydrogels are found to be hemocompatible, cytocompatible and genocompatible. ALPF-NMBA promotes infiltration of L929 fibroblasts and 3D growth of H9c2 cardiomyoblasts and long-term viability.

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Authors and Affiliations

  1. Polymer Science Division, BMT Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, 695 012, Kerala, India

    Finosh Gnanaprakasam Thankam & Jayabalan Muthu

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  1. Finosh Gnanaprakasam Thankam
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  2. Jayabalan Muthu
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Correspondence to Jayabalan Muthu.

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Thankam, F.G., Muthu, J. Infiltration and sustenance of viability of cells by amphiphilic biosynthetic biodegradable hydrogels. J Mater Sci: Mater Med 25, 1953–1965 (2014). https://doi.org/10.1007/s10856-014-5234-0

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  • DOI: https://doi.org/10.1007/s10856-014-5234-0

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