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Phytosynthesis of gold nanoparticles using Mappia foetida leaves extract and their conjugation with folic acid for delivery of doxorubicin to cancer cells

  • Biomaterials Synthesis and Characterization
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Abstract

Mappia foetida leaves extract is used as bioreductant for the synthesis of gold nanoparticles and their application in the efficient delivery of doxorubicin to human cancer cells is reported here. The formation of gold nanoparticles is evident from their characteristic optical absorption at ~560 nm. X-ray diffraction pattern of gold nanoparticles confirmed their fcc structure. Fourier transform infrared spectroscopy shows the bioactive molecules from plant extract capped on the surface of gold nanoparticles and conjugation of doxorubicin along with activated folic acid as navigational molecules for targeted drug delivery. Such a conjugation of gold nanoparticles is characterized by their weight loss, ~35–40 %, due to thermal degradation of plant biomass and conjugated drug along with receptor, as observed in thermogravimetric analysis. The spherical shaped gold nanoparticles (Φ 10–20 nm) are observed by field emission scanning electron microscopy and transmission electron microscopy images and the expected elemental composition by energy dispersive X-ray spectroscopy. Gold nanoparticles conjugated with activated folic acid and doxorubicin complex is found to be toxic for human cancer cells viz., MDA-MB-231, HeLa, SiHa and Hep-G2. Furthermore, the amount of drug released was maximum at pH 5.3 (an ambient condition for intravenous cancer drugs) followed by pH 7.2 and pH 6.8.

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Acknowledgments

Authors thank Dr. S.P. Hegde of Green Gold Global Research Institute, Kumta, Karnataka (India) for experimental facility during this study. Mr. S. Yallappa gratefully acknowledges the financial support from Kuvempu University.

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Authors and Affiliations

  1. Department of Industrial Chemistry, Kuvempu University, Shankaraghatta, 577 451, India

    S. Yallappa

  2. Department of Chemistry, Rani Channamma University, Belagavi, 591 156, India

    J. Manjanna

  3. Toxicology and Drug Discovery Centre for Emerging Technologies, Jain University, Ramanagara, 562 112, India

    B. L. Dhananjaya

  4. SDM Centre for Research in Ayurveda and Allied Sciences, Udupi, 574 118, India

    U. Vishwanatha & B. Ravishankar

  5. Department of Electronics, Kuvempu University, Shankaraghatta, 577 451, India

    H. Gururaj

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  1. S. Yallappa
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Correspondence to J. Manjanna.

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Yallappa, S., Manjanna, J., Dhananjaya, B.L. et al. Phytosynthesis of gold nanoparticles using Mappia foetida leaves extract and their conjugation with folic acid for delivery of doxorubicin to cancer cells. J Mater Sci: Mater Med 26, 235 (2015). https://doi.org/10.1007/s10856-015-5567-3

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