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Electrospun poly(l-lactide)/zein nanofiber mats loaded with Rana chensinensis skin peptides for wound dressing

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Abstract

Electrospun nanofiber mats can display impressive performance as an ideal wound dressing. In this study, poly(l-lactide)(PLLA)/zein nanofiber mats loaded with Rana chensinensis skin peptides (RCSPs) were successfully produced by two different electrospinning techniques, blend and coaxial, with the goal of developing a wound dressing material. The nanofiber mats were investigated by environmental scanning electron microscope (ESEM), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), water contact angle, mechanical tests and cell viability. The resulting nanofiber mats exhibited smooth surfaces, tiny diameters and different cross-sectional shapes from pure PLLA and zein nanofibers. The FTIR result showed that PLLA, zein and RCSPs were well dispersed, without chemical interactions. Compared with coaxial nanofiber mats, blending zein-RCSPs with PLLA enhanced hydrophilicity but decreased mechanical properties. Adding RCSPs into the electrospun nanofibers significantly improved the mechanical properties of the mats. Cell viability studies with human foreskin fibroblasts demonstrated that cell growth on PLLA/zein-RCSPs nanofiber mats was significantly higher than that on PLLA/zein nanofiber mats. The results indicate that nanofiber mats containing RCSPs are potential candidates for wound dressing.

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Acknowledgments

This research was supported by Key Projects in the National Science and Technology Pillar Program during the period of the 12th 5-year Plan (2013BAD16B09). The work was also supported by Key Projects in the Jilin Province Science and Technology Development Plan (20130206043NY).

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Authors and Affiliations

  1. Alan G. MacDiarmid Laboratory, College of Chemistry, Jilin University, Changchun, 130012, China

    Mei Zhang & Xueqi Li

  2. College of Animal Science, Jilin University, Changchun, 130062, China

    Siming Li & Linlin Hao

  3. School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

    Yongjia Liu

Authors
  1. Mei Zhang
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  2. Xueqi Li
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  3. Siming Li
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  4. Yongjia Liu
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  5. Linlin Hao
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Correspondence to Linlin Hao.

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Zhang, M., Li, X., Li, S. et al. Electrospun poly(l-lactide)/zein nanofiber mats loaded with Rana chensinensis skin peptides for wound dressing. J Mater Sci: Mater Med 27, 136 (2016). https://doi.org/10.1007/s10856-016-5749-7

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  • DOI: https://doi.org/10.1007/s10856-016-5749-7

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