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Low temperature growth of ZnO nanotubes for fluorescence quenching detection of DNA

  • Engineering and Nano-engineering Approaches for Medical Devices
  • Original Research
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Abstract

In this work, large-scale and single-crystalline ZnO nanotubes were fabricated by a simple technique from an aqueous solution at a low temperature of 65 °C. According to detailed morphology, structural and compositional analyses showed that the ZnO nanotubes [diameter ~200 nm (wall thickness ~50 nm); length ~1 µm] have single-crystallite with wurtzite structure. As-prepared ZnO nanotubes showed an effective fluorescence quenching for the detection of calf thymus DNA. In particular, increasing DNA concentrations (5–50 µM) into the fixed concentration of ZnO nanotubes (50 µM) progressively quenched the intrinsic fluorescence of nanotubes, which showed that the nanotubes fluorescence was efficiently quenched upon binding to DNA. At the highest ZnO-DNA molar ratios of 1:1.8, around 50.1 % of fluorescence quenching of DNA was observed. Significance of this study provides simple, cost-effective, and low temperature synthesis of ZnO nanotubes revealed better fluorescence property toward a platform of DNA sensor.

Graphical Abstract

ZnO nanotubes with diameter of ~200 nm (wall thickness ~50 nm) and length of about 1 µm prepared at low temperature (65 °C) showed fluorescence was efficiently quenched upon binding to DNA. In particular, around 50.1 % of DNA fluorescence quenching at the highest ZnO-DNA molar ratios of 1:1.8 was observed.

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Acknowledgments

This work was supported by the Internal Research Grant, Alfaisal University (IRG 2014) project No. 4050101011410. The authors gratefully acknowledge the continued support from Alfaisal University and its office of Research.

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Authors and Affiliations

  1. College of Science & General Studies, Alfaisal University, Riyadh, Saudi Arabia

    Faheem Ahmed, Nishat Arshi & Edreese Alsharaeh

  2. School of Materials Science & Engineering, Changwon National University, Changwon, South Korea

    Bon Heun Koo

  3. Department of Applied Physics, Aligarh Muslim University, Aligarh, India

    Saurabh Dwivedi & Ameer Azam

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  1. Faheem Ahmed
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  2. Nishat Arshi
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  3. Saurabh Dwivedi
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Correspondence to Faheem Ahmed or Edreese Alsharaeh.

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Ahmed, F., Arshi, N., Dwivedi, S. et al. Low temperature growth of ZnO nanotubes for fluorescence quenching detection of DNA. J Mater Sci: Mater Med 27, 189 (2016). https://doi.org/10.1007/s10856-016-5805-3

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  • DOI: https://doi.org/10.1007/s10856-016-5805-3

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