Abstract
Critical size defects in the craniofacial region can be effectively treated using three dimensional (3D) composite structures mimicking natural extra cellular matrix (ECM) and incorporated with bioactive ceramics. In this study we have shown that the dynamic liquid bath collector can be used to form electrospun polycaprolactone (PCL)—hydroxyapatite (HA) composite structure as unique 3D scaffold. The structure was found to have three distinct sections (base, stem and head) based on the mechanism of its formation and morphology. The size of the head portion was around 15 mm and was found to vary with the process parameters. Scanning electron microscopy (SEM) analysis revealed that the base had random fibres while the fibres in stem and head sections were aligned but perpendicular to each other. X-ray diffraction (XRD) analysis also showed an increase in the crystallinity index of the fibres from base to head section. Cytotoxicity and cytocompatibility studies using human osteosarcoma (HOS) cells showed good cell adhesion and proliferation indicating the suitability of the 3D structure for craniofacial graft applications.
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Li Y, Chen S, Li L, Qin L, Wang X-L, Lai Y-X. Bone defect animal models for testing efficacy of bone substitute biomaterials. J Orthop Transl. 2015;3:95–104.
Brierly GI, Tredinnick S, Lynham A, Woodruff MA. Critical sized Mandibular defect regeneration in preclinical in vivo models. Curr Mol Biol Rep. 2016;2:83–9.
Loh QL, Choong C. Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng Part B Rev. 2013;19:485–502.
Kneser U, Schaefer DJ, Polykandriotis E, Horch RE. Tissue engineering of bone: the reconstructive surgeon’s point of view. J Cell Mol Med. 2006;10:7–19.
Sun B, Long YZ, Zhang HD, Li MM, Duvail JL, Jiang XY, et al. Advances in three-dimensional nanofibrous macrostructures via electrospinning. Prog Polym Sci. 2014;39:862–90.
Teo WE, Liao S, Chan CK, Ramakrishna S. Remodeling of three-dimensional hierarchically organized nanofibrous assemblies. Curr Nanosci. 2008;4:361–9.
Lee Y-S, Livingston Arinzeh T. Electrospun nanofibrous materials for neural tissue engineering. Polymers. 2011;3:413–26.
Choi JS, Lee SJ, Christ GJ, Atala A, Yoo JJ. The influence of electrospun aligned poly(ɛ-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials. 2008;29:2899–906.
Smit E, Buttner U, Sanderson RD. Continuous yarns from electrospun fibers. Polymer. 2005;46:2419–23.
Yokoyama Y, Hattori S, Yoshikawa C, Yasuda Y, Koyama H, Takato T, et al. Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric. Mater Lett. 2009;63:754–6.
Ki CS, Kim JW, Hyun JH, Lee KH, Hattori M, Rah DK, et al. Electrospun three-dimensional silk fibroin nanofibrous scaffold. J Appl Polym Sci. 2007;106:3922–8.
Teo WE, Gopal R, Ramaseshan R, Fujihara K, Ramakrishna S. A dynamic liquid support system for continuous electrospun yarn fabrication. Polymer. 2007;48:3400–5.
Yousefzadeh M, Latifi M, Amani-tehran M, Teo W, Ramakrishna S. A note on the 3D structural design of electrospun nanofibers. J Eng Fiber Fabr. 2012;7:17–23.
Viswanathan G, Murugesan S, Pushparaj V, Nalamasu O, Ajayan PM, Linhardt RJ. Preparation of biopolymer fibers by electrospinning from room temperature ionic liquids. Biomacromolecules. 2006;7:415–8.
Miyauchi M, Miao J, Simmons TJ, Lee JW, Doherty TV, Dordick JS, Linhardt RJ. Conductive cable fibers with insulating surface prepared by coaxial electrospinning of multiwalled nanotubes and cellulose. Biomacromolecules. 2010;11:2440–5.
Kim M, Son J, Lee H, Hwang H, Choi CH, Kim G. Highly porous 3D nanofibrous scaffolds processed with an electrospinning/laser process. Curr Appl Phys. 2014;14:1–7.
Cipitria A, Skelton A, Dargaville TR, Dalton PD, Hutmacher DW. Design, fabrication and characterization of PCL electrospun scaffolds—a review. J Mater Chem. 2011;93:1539–50.
Pişkin E, İşoğlu IA, Bölgen N, Vargel I, Griffiths S, Çavuşoğlu T, Korkusuz P, Guzel E, Cartmell S. In vivo performance of simvastatin-loaded electrospun spiral-wound polycaprolactone scaffolds in reconstruction of cranial bone defects in the rat model. J Biomed Mater Res Part A. 2009;90A:1137–51.
Díaz E, Sandonis I, Valle MB. In vitro degradation of poly(caprolactone)/nHA composites. J Nanomater. 2014;2014:1–8.
Shin S-H, Purevdorj O, Castano O, Planell JA, Kim H-W. A short review: recent advances in electrospinning for bone tissue regeneration. J Tissue Eng. 2012;3:2041731412443530.
Hutmacher D. Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21:2529–43.
Polini A, Pisignano D, Parodi M, Quarto R, Scaglione S. Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. PLoS One. 2011;6:1–8.
Kostakova E, Seps M, Pokorny P, Lukas D. Study of polycaprolactone wet electrospinning process. Express Polym Lett. 2014;8:554–64.
Bordes C, Fréville V, Ruffin E, Marote P, Gauvrit JY, Briançon S, et al. Determination of poly(ε-caprolactone) solubility parameters: application to solvent substitution in a microencapsulation process. Int J Pharm. 2010;383:236–43.
Catledge S, Clem WC, Shrikishen N, Chowdhury S, Stanishevsky V, Koopman M, Vohra YK. An electrospun triphasic nanofibrous scaffold for bone tissue engineering. Biomed Mater. 2007;2:142–50.
Liu JY, Reni L, Wei Q, Wu JL, Liu S, Wang YJ, et al. Fabrication and characterization of polycaprolactone/calcium sulfate whisker composites. Express Polym Lett. 2011;5:742–52.
Rameshbabu N, Rao KP, Kumar TSS. Acclerated microwave processing of nanocrystalline hydroxyapatite. J Mater Sci. 2005;40:6319–23.
Wehrhan F, Amann K, Molenberg A, Lutz R, Neukam FW, Schlegel KA. PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration. Clin Oral Implants Res. 2012;23:805–13.
Hong S, Kim G. Fabrication of size-controlled three-dimensional structures consisting of electrohydrodynamically produced polycaprolactone micro/nanofibers. Appl Phys A Mater Sci Process. 2011;103:1009–14.
Eva K, Michal Š, Pavel P, Jana H, David L. Wet electrospun polycaprolactone fibrous materials. Proc. NANOCON 2013. 2013. Article ID: 1968.
Wang X, Zhao H, Turng L, Li Q. Crystalline morphology of electrospun poly(ε-caprolactone) (PCL) nanofibers. Ind Eng Chem Res. 2013;52:4939–49.
Lee KH, Kim HY, Khil MS, Ra YM, Lee DR. Characterization of nano-structured poly(ε-caprolactone) nonwoven mats via electrospinning. Polymer. 2003;44:1287–94.
Wong SC, Baji A, Leng S. Effect of fiber diameter on tensile properties of electrospun poly(ε-caprolactone). Polymer. 2008;49:4713–22.
Jin L, Wang T, Feng Z-Q, Zhu M, Leach MK, Naim YI, et al. Fabrication and characterization of a novel fluffy polypyrrole fibrous scaffold designed for 3D cell culture. J Mater Chem. 2012;22:18321.
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The authors thank Prof. Seeram Ramakrishna, National University of Singapore, Singapore for his constant encouragement and support.
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Chakrapani, V.Y., Kumar, T.S.S., Raj, D.K. et al. Electrospun 3D composite scaffolds for craniofacial critical size defects. J Mater Sci: Mater Med 28, 119 (2017). https://doi.org/10.1007/s10856-017-5933-4
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DOI: https://doi.org/10.1007/s10856-017-5933-4