Abstract
In this short paper, we reported the enhanced fluorescence from a single fluorophore bound to a 50nm silver nanoparticle. We found that on average the Cy5 molecules bound to metal nanoparticles are approximately 15-fold brighter than that of free dyes, and that single molecule lifetimes are shorter as compared to free fluorophores. The increased emission rate is primarily the result of local plasmon enhancement. These results demonstrate that the use of fluorophore-metal interactions can increase the brightness and photostability of fluorophores for single molecule detection.
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Peterman EJG, Brasselet S, Moerner WE (1999) The fluorescence dynamics of single molecules of green fluorescent protein. J Phy Chem A 103:10553–10560
Weiss S (1999) Fluorescence spectroscopy of single biomolecules. Science 283:1676–1683
Garcia-Parajo MF, Veerman J, Bouwhuis R, Vallee R, van Hulst NF (2001) Optical probing of single fluorescent molecules and proteins. Chem Phys Che 2:347–360
Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanaocrystas as fluorescent biological labels. Science 281:2013–2016
Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR (2003) Fluroescence spectral properties of cyanine dye-labeled DNA oliogmers on surfaces coated with silver particles anal. Biochem 317:136–146
Malicka J, Gryczynski I, Fang J, Kusba J, Lakowciz JR (2002) Photostability of Cy3 and Cy5-labeled DNA in the presence of metallic silver particles. J Fluoresc 12:439–447
Lakowicz JR, Shen Y, D’Auria S, Malicka J, Fang J, Gryczynski Z, Gryczynski I (2002) Radiative decay engineering. 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer. Anal Biochem 301:111–116
Xie XS, Trautman JK (1998) Optical studies of single molecules at room temperature. Annu Rev Phys Chem 49:441–480
Thomas M, Greffet J-J, Carminati R, Arias-Gonzalez JR (2004) Single-molecule spontaneous emission close to absorbing nanostructures. Appl Phys Lett 85:3863–3865
Chen S, Wang Z, Ballato J, Foulger S, Carroll D (2003) Monopod, bipod, tripod, and tetrapod gold nanocrystals. J Am Chem Soc 125:16186–16187
Orendorff CJ, Murphy CJ (2006) Quantitation of metal content in the silver-assisted growth of gold nanorods. J Phys Chem B 110:3990–3994
Feldheim D, Foss C (2002) Metal nanoparticles: synthesis, characterization and applications. Marcel Dekker, New York
Zhang J, Fu Y, Lakowicz JR (2007) Enhanced Förster Resonance Energy Transfer (FRET) on a single metal particle. J Phys Chem C 111:50–56
Weston KD, Carson PJ, Metiu H, Buratto SK (1998) Room-temperature fluorescence characteristics of single dye molecules adsorbed on a glass surface. J Chem Phys 109(17):7474
Kramer A, Trabesinger W, Hecht B, Wild UP (2002) Optical near-field enhancement at a metal tip probed by a single fluorophores. Appl Phys Lett 80(9):1652
Kühn S, Håkanson U, Rogobete L, Sandoghdar V (2006) Enhancement of single-molecule fluorescence using a gold nanoparticle as an optical nanoantenna. Phys Rev Lett 97:017402
Sokolov K, Chumanov G, Cotton TM (1998) Enhancement of molecular fluorescence near the surface of colloidal metal films anal. Chem 70:3898–3905
Enderlein J (1999) Single-molecule fluorescence near a metal layer. Chem Phys 24:71–79
Enderlein J (2000) A theoretical investigation of single–molecule fluorescence detection on thin metallic layers. Biophys J 78:2151–2158
Bharadwaj P, Anger P, Novotny L (2007) Nanoplasmonic enhancement of single-molecule fluorescence. Nanotechnology 18:044017
Anger P, Bharadwaj P, Novotny L (2006) Enhancement and quenching of single-molecule fluorescence. Phys Rev Lett 96:11302
Lakowicz JR, Malika J, Gryczynski I, Gryczynski Z, Geddes C (2003) Radiative decay engineering: the role of photonic mode density in biotechnology. J Phys D: Appl Phys 36:R240–R249
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This research was supported by a grant from NIH, HG-02655.
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Fu, Y., Zhang, J. & Lakowicz, J.R. Plasmonic Enhancement of Single-Molecule Fluorescence Near a Silver Nanoparticle. J Fluoresc 17, 811–816 (2007). https://doi.org/10.1007/s10895-007-0259-0
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DOI: https://doi.org/10.1007/s10895-007-0259-0