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FITC Labeled Silica Nanoparticles as Efficient Cell Tags: Uptake and Photostability Study in Endothelial Cells

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Abstract

The use of fluorescent nanomaterials has gained great importance in the field of medical imaging. Many traditional imaging technologies have been reported utilizing dyes in the past. These methods face drawbacks due to non-specific accumulation and photobleaching of dyes. We studied the uptake and internalization of two different sized (30 nm and 100 nm) FITC labeled silica nanoparticles in Human umbilical vein endothelial cell line. These nanomaterials show high biocompatability and are highly photostable inside live cells for increased period of time in comparison to the dye alone. To our knowledge, we report for the first time the use of 30 nm fluorescent silica nanoparticles as efficient endothelial tags along with the well studied 100 nm particles. We also have emphasized the good photostability of these materials in live cells.

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Acknowledgement

Srivani Veeranarayanan, Aby Cheruvathoor Poulose, Sheikh Mohamed and Athulya Aravind thank Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan for providing financial support, the Monbukagakusho fellowship. Authors thank Prof. Fukushima for Photoluminescence measurement and Dr. Iwai for guiding in Flow cytometry analysis.

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Authors and Affiliations

  1. Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan

    Srivani Veeranarayanan, Aby Cheruvathoor Poulose, Sheikh Mohamed, Athulya Aravind, Yutaka Nagaoka, Yasuhiko Yoshida, Toru Maekawa & D. Sakthi Kumar

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  1. Srivani Veeranarayanan
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  2. Aby Cheruvathoor Poulose
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  3. Sheikh Mohamed
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  4. Athulya Aravind
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  5. Yutaka Nagaoka
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  6. Yasuhiko Yoshida
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  7. Toru Maekawa
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  8. D. Sakthi Kumar
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Correspondence to D. Sakthi Kumar.

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Veeranarayanan, S., Cheruvathoor Poulose, A., Mohamed, S. et al. FITC Labeled Silica Nanoparticles as Efficient Cell Tags: Uptake and Photostability Study in Endothelial Cells. J Fluoresc 22, 537–548 (2012). https://doi.org/10.1007/s10895-011-0991-3

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  • DOI: https://doi.org/10.1007/s10895-011-0991-3

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