Abstract
Chitosan nano-composite film crosslinked by citric acid and with glycerol as plasticizer and MgO as antibacterial agent was prepared by casting method. MgO nanoparticles were synthesized via calcination method in furnace at 500 °C for 4 h and characterized by X-ray diffraction and transmission electron microscope. The chitosan nano-composite film with composition chitosan/citric/glycerol/magnesium oxide (1 wt%:1 wt%:75 vol%:10 wt%) has high mechanical properties than other films. The effects of different irradiation doses on the mechanical, thermal and antibacterial activity were investigated. The tensile strength enhanced by increasing irradiation dose up to 10 kGy and the elongation negligible changed as irradiation dose increased. The thermal stability slightly increased up to dose 2.5 kGy then decreased with dose increment. The antimicrobial activity film was studied against white mulberry-borne bacterial pathogens either Gram positive or Gram negative bacteria and has positive impact of gamma irradiation on the antimicrobial activity. The use of the selected chitosan nano-composite film which irradiated by dose of 2.5 kGy and has magnesium oxide of average particle size 54.3 nm as new packaging materials found to improve storage quality and shelf-life of mulberry fruit.
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Vasile C, Kulshreshtha AK (2003) Handbook of polymer blends and composites, vol 4. Rapra Technology Ltd., Shrewsbury
Husseinsyah S, Azmin AN, Ismail H (2013) Effect of maleic anhydride-grafted-polyethylene (MAPE) and silane on properties of recycled polyethylene/chitosan biocomposites. Polym-Plast Technol Eng 52:168–174
Cui Z, Beach ES, Anastas PT (2011) Modification of chitosan films with environmentally benign reagents for increased water resistance. Green Chem Lett Rev 4:35–40
Shu XZ, Zhu KJ, Weihong S (2001) Novel pH-sensitive citrate cross-linked chitosan film for drug controlled release. Int J Pharm 212:19–28
Gawish SM, Abo El-Ola SM, Ramadan AM, Abou El-Kheir AA (2012) Used as a crosslinking agent for the grafting of chitosan onto woolen fabric. J Appl Polym Sci 123:3345–3353
Sundrarajan M, Suresh J, Rajiv Gandhi R (2012) A comparative study on antibacterial properties of MgO nanoparticles prepared under different calcination temperature. Dig J Nanomater Biostruct 7:983–989
Awwad AM, Ahmad AL (2014) Biosynthesis, characterization, and optical properties of magnesium hydroxide and oxide nanoflakes using citrus limon leaf extract. Arab J Phys Chem 1:65–70
Abdel Moez A, Aly SS, Elshaer YH (2012) Effect of gamma radiation on low density polyethylene (LDPE) films: optical, dielectric and FTIR studies. Spectrochim Acta A 93:203–207
Muzzarelli RAA (1996) Chitosan-based dietary foods. Carbohydr Polym 29:309–316
Rosiak J, Ulanski P, Kucharska M, Dutkiewicz J, Judkiewicz L (1992) Radiation sterilization of chitosan sealant for vascular prostheses. J Radioanal Nucl Chem 1:87–96
Desai KG, Jin H (2006) Study of gamma-irradiation effects on chitosan microparticles. Drug Deliv 13:39–50
Haldorai Y, Shim J-J (2014) An efficient removal of methyl orange dye from aqueous solution by adsorption onto chitosan/MgO composite: a novel reusable adsorbent. Appl Surf Sci 292:447–453
Sibi S, Pragathiswaran C, Venkatesan S, Anthuvan BS, Kullagounder S (2016) Chitosan and reinforced chitosan films for the removal of Cr(VI) heavy metal from synthetic aqueous solution. Orient J Chem 32:671–680
Patel MK, Ali MA, Agrawal MZafaryab,VV, Rizvi MMA, Ansari ZA, Ansari SG, Malhotra BD (2013) Biocompatible nanostructured magnesium oxide-chitosan platform for genosensing application. Biosens Bioelectron 45:181–188
Sanuja S, Agalya A, Umapathy MJ (2014) Studies on magnesium oxide reinforced chitosan bionanocomposite incorporated with clove oil for active food packaging application. Int J Polym Mater Polym Biomater 63:733–740
Kojima Y, Kimura T, Nakagawa K, Asai A, Hasumi K, Oikawa S, Miyazawa T (2010) Effect of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humans. J Clin Biochem Nutr 47:155–161
Lee SH, Jeong E, Paik S,-S, Jeon JH, Jung SW, Kim H-B, Kim M, M-H Chun, I-B Kim (2014). Cyanidin-3-glucoside extracted from mulberry fruit can reduce N-methyl-N-nitrosourea-induced retinal degeneration in rats. Curr Eye Res 39:79–87
Kim SB, Chang BY, Jo YH, Lee SH, Han SB, Hwang BY, Kim SY, MK Lee (2013) Macrophage activating activity of pyrrole alkaloids from Morus alba fruits. J Ethnopharmacol 145:393–396
Niidome T, Takahashi K, Goto Y, Goh S, Tanaka N, Kamei K, Ichida M, Hara S, Akaike A, Kihara T, Sugimoto H (2007) Mulberry leaf extract prevents amyloid beta-peptide fibril formation and neurotoxicity. Neuroreport Neurophysiol Basic Clin 18:813–816
Rashmi MM, Kiran P, Anuradar V (2012) Synthesis and characterization of oxazolidones with improved thermal stability. Adv Appl Sci Res 3:2553–2560
Oxoid manual (1982) The Oxoid manual of culture media, ingredients and other laboratory services, 5th edn. Oxoid limited, Hampshire
El-Bialy HA, Abou El-Nour SA (2015) Physical and chemical stress on Serratia marcescens and studies on prodigiosin pigment production. Ann Microbiol 65:59–68
Vattem DA, Lin YT, Labbe RG, Shetty LK (2004) Phenolic antioxidant mobilization in cranberry pomace by solid-state bioprocessing using food grade fungus Lentinus edodes and effect on antimicrobial activity against selected food borne pathogens. Innov Food Sci Emerg Technol 5:81–86
Chen Z, Zhu C, Han Z (2011) Effects of aqueous chlorine dioxide treatment on nutritional components and shelf-life of mulberry fruit (Morus alba L.). J Biosci Bioeng 111:675–681
Vatsha B, Tetyana P, Shumbula PM, Ngila JC, Sikhwivhilu LM (2013) Effects of precipitation temperature on nanoparticle surface area and antibacterial behaviour of Mg(OH)2 and MgO nanoparticles. J Biomater Nanobiotechnol 4:365–373
Epure V, Griffon M, Pollet E, Avérous L (2011) Structure and properties of glycerol-plasticized chitosan obtained by mechanical kneading. Carbohydr Polym 83:947–952
Cissé M, Montet D, Loiseau G, Ducamp-Collin M-N (2012) Influence of the concentrations of chitosan and glycerol on edible film properties showed by response surface methodology. J Polym Environ 20:830–837
Othman SH (2014) Bio-nanocomposite materials for food packaging applications: types of biopolymer and nano-sized filler. Agric Agric Sci Procedia 2:296–303
Yang F, Li X, Cheng M, Gong Y, Zhao N, Zhang X, Yang Y (2002) Performance modification of chitosan membranes induced by gamma irradiation. J Biomater Appl 16:216–226
García MA, de la Paz N, Castro C, Rodríguez JL, Rapado M, Zuluaga R, Gañán P, Casariego A (2015) Effect of molecular weight reduction by gamma irradiation on the antioxidant capacity of chitosan. J Radiat Res Appl Sci 8:190–200
Sánchez-González L, González-Martínez C, Chiralt A, Cháfer M (2010) Physical and antimicrobial properties of chitosan–tea tree essential oil composite films. J Food Eng 98:443–452
Muzzarelli R, Filippini O, Tarsi R, Giovanetti E, Biagini G, Varaldo PE (1990) Antimicrobial properties of N-carboxybutyl chitosan. Antimicrob Agents Chemother 34:2019–2023
Liu H, Du YM, Wang XH, Sun LP (2004) Chitosan kills bacteria through cell membrane damage. Int J Food Microbiol 95:147–155
Raafat D, Sahl HG (2009) Chitosan and its antimicrobial potential—a critical literature survey. Microb Biotechnol 2:186–201
Yamamoto O, Ohira T, Alvarez K, Fukuda M (2010) Antibacterial characteristics of CaCO3–MgO composites. Mater Sci Eng B 173:208–212
Tang Z-X, Lv B-F (2014) MgO nanoparticles as antibacterial agent: preparation and activity. Braz J Chem Eng 31:591–601
Leung YH, Ng AMC, Xu X, Shen Z, Gethings LA, Wong MT, Chan C et al (2014) Mechanisms of antibacterial activity of MgO: non-ROS mediated toxicity of MgO nanoparticles towards Escherichia coli. Small 10:1171–1183
Falguera V, Quintero JP, Jiménez A, Muñoz JA, Ibarz A (2011) Edible films and coatings: Structures, active functions and trends in their use. Trends Food Sci Technol 22:292–303
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Abdel Ghaffar, A.M., Ali, H.E., Nasef, S.M. et al. Effect of Gamma Radiation on the Properties of Crosslinked Chitosan Nano-composite Film. J Polym Environ 26, 3226–3236 (2018). https://doi.org/10.1007/s10924-018-1208-5
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DOI: https://doi.org/10.1007/s10924-018-1208-5