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Comparative proteomic and radiobiological analyses in human lung adenocarcinoma cells

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Abstract

In clinic, many non-small cell lung cancer (NSCLC) patients receive radiation therapy after chemotherapy failure. However, whether the multidrug resistance (MDR) can elevate the radioresistance (RDR) remains unclear. To evaluate the MDR’s effect on the RDR, screen MDR- and RDR-related proteins in human lung adenocarcinoma (HLA) cells and tissues A549, and A549/DDP cells after irradiation were analyzed by colony-forming assay and flow cytometry. Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF–MS) were utilized to identify differentially expressed proteins (DEPs) between them. The value of D0, Dq, and SF2 increased, the mean percentage in G2 phase and apoptosis rate significantly decreased in A549/DDP cells compared with A549 cells. 40 DEP points were found, and among them 27 were identified through proteomics. Four up-regulated proteins (HSPB1, Vimentin, Cofilin-1, and Annexin A4) in MDR cells compared with non-MDR cells, were confirmed by Western blot. Immuno-histochemistry showed that they were also over-expressed in MDR tissues compared with non-MDR counterparts of HLA. These results proved that the MDR in HLA cells and tissues increased the RDR. HSPB1, Vimentin, Cofilin-1, and Annexin A4 are potential biomarkers for predicting HLA response to MDR and RDR, and novel treatment targets of HLA.

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Acknowledgments

This study was supported by a grant (No.2010FJ3039) from the Department of Science and Technology of Hunan province of China, and Build-up of Key Disciplines of Education Department of Hunan province of China (No.2009).

Competing interests

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. Department of Oncology, Xingya Hospital, Central South University, Changsha, 410008, China

    Rui Wei, Liangfang Shen, Wuzhong Jiang, Meizuo Zhong, Yun Xie, Dingyi Yang, Lili He & Qing Zhou

  2. Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China

    Yingjin Zhang

  3. Key Laboratory of Cancer Proteomics, Ministry of Health of China, Xingya Hospital, Central South University, Changsha, 410008, China

    Cui Li

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  1. Rui Wei
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  10. Qing Zhou
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Correspondence to Yingjin Zhang.

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Wei, R., Zhang, Y., Shen, L. et al. Comparative proteomic and radiobiological analyses in human lung adenocarcinoma cells. Mol Cell Biochem 359, 151–159 (2012). https://doi.org/10.1007/s11010-011-1008-x

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  • DOI: https://doi.org/10.1007/s11010-011-1008-x

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