Abstract
The paper describes three Penicillium verruculosum 28K mutants with about threefold enhanced production of five industrially important carbohydrases. The two-stage fermentation process that we developed provided a further two- to threefold increase in the production of carbohydrases. Physiological and biochemical studies showed that the synthesis of all five carbohydrases is inducible. Carboxymethylcellulase, xylanase, and β-glucanase are synthesized under a common regulatory control, as is evident from the concurrent increase in the synthesis of these enzymes in the presence of microcrystalline cellulose. The synthesis of avicelase and β-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
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REFERENCES
Bhat, M.K. and Bhat, S., Cellulose-Degrading Enzymes and Their Potential Industrial Applications, Biotechnol. Adv., 1997, vol. 15, pp. 583–620.
Jain, S., Parriche, M., Durand, H., and Tiraby, G., Production of Polysaccharidases by a Cellulase-Pectinase Hyperproducing Mutant (Pol 6) of Penicillium occitanis, Enzyme Microb. Technol., 1990, vol. 12, pp. 691–695.
Chaabouni, S.E., Belguith, H., Hassairi, I., Rad, M., and Ellouz, R., Optimization of Cellulase Production by Penicillium occitanis, Appl. Microb. Biotechnol., 1995, vol. 43, pp. 267–269.
Brown, J.A., Collin, S.A., and Wood, T.M., Enhanced Enzyme Production by the Cellulolytic Fungus Penicillium pinophilum, Mutant Strain NTG III/6, Enzyme Microb. Technol., 1987, vol. 9, pp. 176–179.
Lachke, A.H., Bastawde, K.B., Powar, V.K., and Srinivasant, M.C., Isolation of Hypercellulolytic Mutant (Cu-1) of Penicillium funiculosum, Enzyme Microb. Technol., 1988, vol. 8, pp. 105–108.
Gomarteli, M., Kvesitadze, E., Bezborodov, A.M., Shkol’nyi, A.T., Dzhobava, M., and Adeishvili, E., Mutants of Penicillium canescens with Enhanced Xylanase Activity, Prikl. Biokhim. Mikrobiol., 1998, vol. 34, pp. 650–654.
Kastel’yanos, O.F., Ermolova, O.V., Sinitsyn, A.P., Popova, N.N., Okunev, O.N., Kerns, G., and Kude, E., Purification of Enzymes of the Cellulase Complex of Penicillium verruculosum and the Study of Their Biochemical Properties and Specificity, Biokhimiya, 1995, vol. 60, pp. 925–943.
Berlin, A.G., Gusakov, A.V., Sinitsyna, O.A., and Sinitsin, A.P., Detection of Major Xylanase-Containing Cellulose-Binding Domain from Penicillium verruculosum by Combination of Chromatofocusing and Limited Proteolysis, Appl. Biochem. Biotechnol., 2000, vol. 88, pp. 345–352.
Teather, R.M. and Wood, P.J., Use of Congo-Red Polysaccharide Interaction in Enumeration and Characterization of Cellulolytic Bacteria from the Bovine Rumen, Appl. Environ. Microbiol., 1982, vol. 43, pp. 777–780.
Sinitsyn, A.P., Chernoglazov, V.M., and Gusakov, A.V., Metody opredeleniya aktivnosti tsellyulaz, Itogi Nauki Tekh., Ser. Biotekhnol., 1993, vol. 25, pp. 38–64.
Nelson, A., A Photometric Adaptation of the Determination of Reducing Sugars, J. Biol. Chem., 1944, vol. 153, pp. 375–380.
Somogy, M., A New Reagent for the Determination of Sugars, J. Biol. Chem., 1945, vol. 160, pp. 61–68.
Bailey, M.J. and Poutanen, K., Production of Xylanolytic Enzymes by Strains of Aspergillus, Appl. Microbiol. Biotechnol., 1989, vol. 30, pp. 5–10.
Rao, M., Gaikwad, S., Mishra, C., and Deshpande, V., Induction and Catabolite Repression of Cellulase in Penicillium funiculosum, Appl. Biochem. Biotechnol., 1988, vol. 19, pp. 129–137.
Senior, D.J., Mayers, P.R., and Saddler, J.N., Xylanase Production by Trichoderma harzianum E58, Appl. Microbiol. Biotechnol., 1989, vol. 32, pp. 137–142.
Hrmova, M., Biely, P., and Vrsanska, M., Cellulose-and Xylan-Degrading Enzymes of Aspergillus terreus and Aspergillus niger, Enzyme Microb. Technol., 1989, vol. 11, pp. 610–616.
Nevalainen, H. and Pentilla, M., Molecular Biology of Cellulolytic Fungi, The Mycota: II. Genetic and Biotechnology, Esser, K. and Lemke, P.A., Eds., Berlin: Springer, 1995.
Kurasawa, T., Yachi, M., Suto, M., Kamagata, Y., Takao, S., and Tomita, F., Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum, Appl. Environ. Microbiol., 1992, vol. 58, pp. 106–110.
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Translated from Mikrobiologiya, Vol. 74, No. 2, 2005, pp. 172–178.
Original Russian Text Copyright © 2005 by Solov’eva, Okunev, Vel’kov, Koshelev, Bubnova, Kondrat’eva, Skomarovskii, Sinitsyn.
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Solov’eva, I.V., Okunev, O.N., Vel’kov, V.V. et al. The selection and properties of Penicillium verruculosum mutants with enhanced production of cellulases and xylanases. Microbiology 74, 141–146 (2005). https://doi.org/10.1007/s11021-005-0043-6
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DOI: https://doi.org/10.1007/s11021-005-0043-6