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Sciatic nerve regeneration by transplantation of menstrual blood-derived stem cells

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Abstract

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via a neural guidance conduit, for peripheral nerve regeneration. The synthesized poly (ɛ-caprolactone)/Gelatin conduit, filled with collagen type I and seeded with 3 × 104 MenSCs, was implanted into a rat’s 10 mm sciatic nerve defect. The results of hot plate latency, sciatic functional index and weight-loss percentage of wet gastrocnemius muscle demonstrated that the MenSC transplantation had comparable nerve regeneration outcome to autograft, as the gold standard of nerve bridging. The transplantation of MenSCs via a synthetic conduit could ameliorate the functional recovery of sciatic nerve-injured rats which make them a potential candidate for cell therapy of peripheral nervous system disorders.

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Author notes

  1. Saeed Farzamfar and Mahdi Naseri-Nosar are co-first authors who contributed equally to this work.

Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran

    Saeed Farzamfar

  2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran

    Mahdi Naseri-Nosar & Ahmad Vaez

  3. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, 141556447, Iran

    Alireza Ghanavatinejad

  4. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, 117719615, Iran

    Amir Hassan Zarnani

  5. Immunology Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran

    Amir Hassan Zarnani

  6. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, 3613773955, Iran

    Majid Salehi

Authors
  1. Saeed Farzamfar
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  2. Mahdi Naseri-Nosar
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  3. Alireza Ghanavatinejad
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  4. Ahmad Vaez
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  5. Amir Hassan Zarnani
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  6. Majid Salehi
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Corresponding authors

Correspondence to Amir Hassan Zarnani or Majid Salehi.

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Farzamfar, S., Naseri-Nosar, M., Ghanavatinejad, A. et al. Sciatic nerve regeneration by transplantation of menstrual blood-derived stem cells. Mol Biol Rep 44, 407–412 (2017). https://doi.org/10.1007/s11033-017-4124-1

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  • DOI: https://doi.org/10.1007/s11033-017-4124-1

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