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Erschienen in: Journal of Nanoparticle Research 6/2008

01.08.2008 | FOCUS ON NANOMANUFACTURING

Preparation of nanoparticles by continuous-flow microfluidics

verfasst von: Andreas Jahn, Joseph E. Reiner, Wyatt N. Vreeland, Don L. DeVoe, Laurie E. Locascio, Michael Gaitan

Erschienen in: Journal of Nanoparticle Research | Ausgabe 6/2008

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Abstract

We review a variety of micro- and nanoparticle formulations produced with microfluidic methods. A diverse variety of approaches to generate microscale and nanoscale particles has been reported. Here we emphasize the use of microfluidics, specifically microfluidic systems that operate in a continuous flow mode, thereby allowing continuous generation of desired particle formulations. The generation of semiconductor quantum dots, metal colloids, emulsions, and liposomes is considered. To emphasize the potential benefits of the continuous-flow microfluidic methodology for nanoparticle generation, preliminary data on the size distribution of liposomes formed using the microfluidic approach is compared to the traditional bulk alcohol injection method.

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Literatur
Zurück zum Zitat Abraham SA et al (2005) The liposomal formulation of doxorubicin. Methods Enzymol 391:71–97CrossRef Abraham SA et al (2005) The liposomal formulation of doxorubicin. Methods Enzymol 391:71–97CrossRef
Zurück zum Zitat Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271(5251):933–937CrossRef Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271(5251):933–937CrossRef
Zurück zum Zitat Andresen TL, Jensen SS, Jorgensen K (2005) Advanced strategies in liposomal cancer therapy: problems and prospects of active and tumor specific drug release. Prog Lipid Res 44(1):68–97CrossRef Andresen TL, Jensen SS, Jorgensen K (2005) Advanced strategies in liposomal cancer therapy: problems and prospects of active and tumor specific drug release. Prog Lipid Res 44(1):68–97CrossRef
Zurück zum Zitat Anna SL, Bontoux N, Stone HA (2003) Formation of dispersions using flow focusing in microchannels. Appl Phys Lett 82(3):364–366CrossRef Anna SL, Bontoux N, Stone HA (2003) Formation of dispersions using flow focusing in microchannels. Appl Phys Lett 82(3):364–366CrossRef
Zurück zum Zitat Ayyagari AL et al (2006) Long-circulating liposomal contrast agents for magnetic resonance imaging. Mag Reson Med 55(5):1023–1029CrossRef Ayyagari AL et al (2006) Long-circulating liposomal contrast agents for magnetic resonance imaging. Mag Reson Med 55(5):1023–1029CrossRef
Zurück zum Zitat Batzri S, Korn ED (1973) Single bilayer liposomes prepared without sonication. Biochim Biophys Acta 298(4):1015–1019CrossRef Batzri S, Korn ED (1973) Single bilayer liposomes prepared without sonication. Biochim Biophys Acta 298(4):1015–1019CrossRef
Zurück zum Zitat Bessoth FG, deMello AJ, Manz A (1999) Microstructure for efficient continuous flow mixing. Anal Commun 36(6):213–215CrossRef Bessoth FG, deMello AJ, Manz A (1999) Microstructure for efficient continuous flow mixing. Anal Commun 36(6):213–215CrossRef
Zurück zum Zitat Brazhnik KP et al (2005) Directed growth of pure phosphatidylcholine nanotubes in microfluidic channels. Langmuir 21(23):10814–10817CrossRef Brazhnik KP et al (2005) Directed growth of pure phosphatidylcholine nanotubes in microfluidic channels. Langmuir 21(23):10814–10817CrossRef
Zurück zum Zitat Chan EM, Alivisatos AP, Mathies RA (2005) High-temperature microfluidic synthesis of CdSe nanocrystals in nanoliter droplets. J Am Chem Soc 127(40):13854–13861CrossRef Chan EM, Alivisatos AP, Mathies RA (2005) High-temperature microfluidic synthesis of CdSe nanocrystals in nanoliter droplets. J Am Chem Soc 127(40):13854–13861CrossRef
Zurück zum Zitat Chan EM, Mathies RA, Alivisatos AP (2003) Size-controlled growth of CdSe nanocrystals in microfluidic reactors. Nano Lett 3(2):199–201CrossRef Chan EM, Mathies RA, Alivisatos AP (2003) Size-controlled growth of CdSe nanocrystals in microfluidic reactors. Nano Lett 3(2):199–201CrossRef
Zurück zum Zitat Christopher GF et al (2007) Microfluidic methods for generating continuous droplet streams. J Phys D Appl Phys 40:R319–R336CrossRef Christopher GF et al (2007) Microfluidic methods for generating continuous droplet streams. J Phys D Appl Phys 40:R319–R336CrossRef
Zurück zum Zitat Cottam BF et al (2007) Accelerated synthesis of titanium oxide nanostructures using microfluidic chips. Lab Chip 7(2):167–169CrossRef Cottam BF et al (2007) Accelerated synthesis of titanium oxide nanostructures using microfluidic chips. Lab Chip 7(2):167–169CrossRef
Zurück zum Zitat Crosasso P et al (2000) Preparation, characterization and properties of sterically stabilized paclitaxel-containing liposomes. J Control Release 63(1–2):19–30CrossRef Crosasso P et al (2000) Preparation, characterization and properties of sterically stabilized paclitaxel-containing liposomes. J Control Release 63(1–2):19–30CrossRef
Zurück zum Zitat Dittrich PS et al (2006) On-chip extrusion of lipid vesicles and tubes through microsized apertures. Lab Chip 6(4):488–493CrossRef Dittrich PS et al (2006) On-chip extrusion of lipid vesicles and tubes through microsized apertures. Lab Chip 6(4):488–493CrossRef
Zurück zum Zitat Edel JB et al (2002) Microfluidic routes to the controlled production of nanoparticles. Chem Commun 10:1136–1137 Edel JB et al (2002) Microfluidic routes to the controlled production of nanoparticles. Chem Commun 10:1136–1137
Zurück zum Zitat Garstecki P et al (2006) Formation of droplets and bubbles in a microfluidic T-junction - scaling and mechanism of break-up. Lab Chip 6(3):437–446CrossRef Garstecki P et al (2006) Formation of droplets and bubbles in a microfluidic T-junction - scaling and mechanism of break-up. Lab Chip 6(3):437–446CrossRef
Zurück zum Zitat Gulsen D, Li CC, Chauhan A (2005) Dispersion of DMPC liposomes in contact lenses for ophthalmic drug delivery. Curr Eye Res 30(12):1071–1080CrossRef Gulsen D, Li CC, Chauhan A (2005) Dispersion of DMPC liposomes in contact lenses for ophthalmic drug delivery. Curr Eye Res 30(12):1071–1080CrossRef
Zurück zum Zitat Hung LH et al (2006) Alternating droplet generation and controlled dynamic droplet fusion in microfluidic device for CdS nanoparticle synthesis. Lab Chip 6(2):174–178CrossRef Hung LH et al (2006) Alternating droplet generation and controlled dynamic droplet fusion in microfluidic device for CdS nanoparticle synthesis. Lab Chip 6(2):174–178CrossRef
Zurück zum Zitat Ishida T, Harashima H, Kiwada H (2002) Liposome clearance. Biosci Rep 22(2):197–224CrossRef Ishida T, Harashima H, Kiwada H (2002) Liposome clearance. Biosci Rep 22(2):197–224CrossRef
Zurück zum Zitat Jahn A et al (2007) Microfluidic directed formation of liposomes of controlled size. Langmuir 23(11):6289–6293CrossRef Jahn A et al (2007) Microfluidic directed formation of liposomes of controlled size. Langmuir 23(11):6289–6293CrossRef
Zurück zum Zitat Jahn A et al (2004) Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing. J Am Chem Soc 126(9):2674–2675CrossRef Jahn A et al (2004) Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing. J Am Chem Soc 126(9):2674–2675CrossRef
Zurück zum Zitat Jensen KF (2001) Microreaction engineering - is small better? Chem Eng Sci 56(2):293–303CrossRef Jensen KF (2001) Microreaction engineering - is small better? Chem Eng Sci 56(2):293–303CrossRef
Zurück zum Zitat Johnson TJ, Ross D, Locascio LE (2002) Rapid microfluidic mixing. Anal Chem 74(1):45–51CrossRef Johnson TJ, Ross D, Locascio LE (2002) Rapid microfluidic mixing. Anal Chem 74(1):45–51CrossRef
Zurück zum Zitat Kelly BT et al (2007) Miniaturizing chemistry and biology in microdroplets. Chem Commun (18):1773–1788 Kelly BT et al (2007) Miniaturizing chemistry and biology in microdroplets. Chem Commun (18):1773–1788
Zurück zum Zitat Khan SA et al (2004) Microfluidic synthesis of colloidal silica. Langmuir 20(20):8604–8611CrossRef Khan SA et al (2004) Microfluidic synthesis of colloidal silica. Langmuir 20(20):8604–8611CrossRef
Zurück zum Zitat Kikuchi H et al (1999) Gene delivery using liposome technology. J Control Release 62(1–2):269–277CrossRef Kikuchi H et al (1999) Gene delivery using liposome technology. J Control Release 62(1–2):269–277CrossRef
Zurück zum Zitat Knight JB et al (1998) Hydrodynamic focusing on a silicon chip: mixing nanoliters in microseconds. Phys Rev Lett 80(17):3863–3866CrossRef Knight JB et al (1998) Hydrodynamic focusing on a silicon chip: mixing nanoliters in microseconds. Phys Rev Lett 80(17):3863–3866CrossRef
Zurück zum Zitat Kremer JMH et al (1977) Vesicles of variable diameter prepared by a modified injection method. Biochemistry 16(17):3932–3935CrossRef Kremer JMH et al (1977) Vesicles of variable diameter prepared by a modified injection method. Biochemistry 16(17):3932–3935CrossRef
Zurück zum Zitat Kuribayashi K et al (2006) Electroformation of giant liposomes in microfluidic channels. Meas Sci Technol 17(12):3121–3126CrossRef Kuribayashi K et al (2006) Electroformation of giant liposomes in microfluidic channels. Meas Sci Technol 17(12):3121–3126CrossRef
Zurück zum Zitat LaMer VK, Dinegar RH (1950) Theory, production and mechanism of formation of monodispersed hydrosols. J Am Chem Soc 72(11):4847–4854CrossRef LaMer VK, Dinegar RH (1950) Theory, production and mechanism of formation of monodispersed hydrosols. J Am Chem Soc 72(11):4847–4854CrossRef
Zurück zum Zitat Lasic DD (1988) The mechanism of vesicle formation. Biochem J 256(1):1–11 Lasic DD (1988) The mechanism of vesicle formation. Biochem J 256(1):1–11
Zurück zum Zitat Lin XZ, Terepka AD, Hong Y (2004) Synthesis of silver nanoparticles in a continuous flow tubular microreactor. Nano Lett 4(11):2227–2232CrossRef Lin XZ, Terepka AD, Hong Y (2004) Synthesis of silver nanoparticles in a continuous flow tubular microreactor. Nano Lett 4(11):2227–2232CrossRef
Zurück zum Zitat Lin Y-C, Li M, Wang Y-T, Lai T-H, Chaing J-T, Huang K-S (2005) A new method for the preparation of self-assembled phospholipid microtubes using microfluidic technology. Seoul, Korea, pp 1592–1595 Lin Y-C, Li M, Wang Y-T, Lai T-H, Chaing J-T, Huang K-S (2005) A new method for the preparation of self-assembled phospholipid microtubes using microfluidic technology. Seoul, Korea, pp 1592–1595
Zurück zum Zitat Link DR et al (2004) Geometrically mediated breakup of drops in microfluidic devices. Phys Rev Lett 92(5):Art. No. 054503 Link DR et al (2004) Geometrically mediated breakup of drops in microfluidic devices. Phys Rev Lett 92(5):Art. No. 054503
Zurück zum Zitat Litzinger DC et al (1994) Effect of liposome size on the circulation time and intraorgan distribution of amphipathic poly(ethylene glycol)-containing liposomes. Biochim Biophys Acta Biomembr 1190(1):99–107CrossRef Litzinger DC et al (1994) Effect of liposome size on the circulation time and intraorgan distribution of amphipathic poly(ethylene glycol)-containing liposomes. Biochim Biophys Acta Biomembr 1190(1):99–107CrossRef
Zurück zum Zitat Luan WL et al (2007) Open-to-air synthesis of monodisperse CdSe nanocrystals via microfluidic reaction and its kinetics. Nanotechnology 18(17):175603 (6 pp) Luan WL et al (2007) Open-to-air synthesis of monodisperse CdSe nanocrystals via microfluidic reaction and its kinetics. Nanotechnology 18(17):175603 (6 pp)
Zurück zum Zitat Mamot C et al (2003) Liposome-based approaches to overcome anticancer drug resistance. Drug Resist Updat 6(5):271–279CrossRef Mamot C et al (2003) Liposome-based approaches to overcome anticancer drug resistance. Drug Resist Updat 6(5):271–279CrossRef
Zurück zum Zitat Martina MS et al (2005) Generation of superparamagnetic liposomes revealed as highly efficient MRI contrast agents for in vivo imaging. J Am Chem Soc 127(30):10676–10685CrossRef Martina MS et al (2005) Generation of superparamagnetic liposomes revealed as highly efficient MRI contrast agents for in vivo imaging. J Am Chem Soc 127(30):10676–10685CrossRef
Zurück zum Zitat Maulucci G et al (2005) Particle size distribution in DMPC vesicles solutions undergoing different sonication times. Biophys J 88(5):3545–3550CrossRef Maulucci G et al (2005) Particle size distribution in DMPC vesicles solutions undergoing different sonication times. Biophys J 88(5):3545–3550CrossRef
Zurück zum Zitat Mayer LD et al (2000) Designing liposomal anticancer drug formulations for specific therapeutic applications. J Liposome Res 10(2–3):99–115CrossRef Mayer LD et al (2000) Designing liposomal anticancer drug formulations for specific therapeutic applications. J Liposome Res 10(2–3):99–115CrossRef
Zurück zum Zitat Medintz IL et al (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4(6):435–446CrossRef Medintz IL et al (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4(6):435–446CrossRef
Zurück zum Zitat Michalet X et al (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307(5709):538–544CrossRef Michalet X et al (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307(5709):538–544CrossRef
Zurück zum Zitat Mulder WJM et al (2006) Lipid-based nanoparticles for contrast-enhanced MRI and molecular imaging. NMR Biomed 19(1):142–164CrossRef Mulder WJM et al (2006) Lipid-based nanoparticles for contrast-enhanced MRI and molecular imaging. NMR Biomed 19(1):142–164CrossRef
Zurück zum Zitat Nakamura H et al (2004) Application of a microfluidic reaction system for CdSe nanocrystal preparation: their growth kinetics and photoluminescence analysis. Lab Chip 4(3):237–240CrossRef Nakamura H et al (2004) Application of a microfluidic reaction system for CdSe nanocrystal preparation: their growth kinetics and photoluminescence analysis. Lab Chip 4(3):237–240CrossRef
Zurück zum Zitat Pavelic Z et al (2005) Development and in vitro evaluation of a liposomal vaginal delivery system for acyclovir. J Control Release 106(1–2):34–43CrossRef Pavelic Z et al (2005) Development and in vitro evaluation of a liposomal vaginal delivery system for acyclovir. J Control Release 106(1–2):34–43CrossRef
Zurück zum Zitat Puntes VF, Krishnan KM, Alivisatos AP (2001) Colloidal nanocrystal shape and size control: the case of cobalt. Science 291(5511):2115–2117CrossRef Puntes VF, Krishnan KM, Alivisatos AP (2001) Colloidal nanocrystal shape and size control: the case of cobalt. Science 291(5511):2115–2117CrossRef
Zurück zum Zitat Ramachandran S et al (2006) Cisplatin nanoliposomes for cancer therapy: AFM and fluorescence Imaging of cisplatin encapsulation, stability, cellular uptake, and toxicity. Langmuir 22(19):8156–8162CrossRef Ramachandran S et al (2006) Cisplatin nanoliposomes for cancer therapy: AFM and fluorescence Imaging of cisplatin encapsulation, stability, cellular uptake, and toxicity. Langmuir 22(19):8156–8162CrossRef
Zurück zum Zitat Reiss P, Bleuse J, Pron A (2002) Highly luminescent CdSe/ZnSe core/shell nanocrystals of low size dispersion. Nano Lett 2(7):781–784CrossRef Reiss P, Bleuse J, Pron A (2002) Highly luminescent CdSe/ZnSe core/shell nanocrystals of low size dispersion. Nano Lett 2(7):781–784CrossRef
Zurück zum Zitat Sadava D, Coleman A, Kane SF (2002) Liposomal daunorubicin overcomes drug resistance in human breast, ovarian and lung carcinoma cells. J Liposome Res 12(4):301–309CrossRef Sadava D, Coleman A, Kane SF (2002) Liposomal daunorubicin overcomes drug resistance in human breast, ovarian and lung carcinoma cells. J Liposome Res 12(4):301–309CrossRef
Zurück zum Zitat Saito R et al (2005) Gadolinium-loaded liposomes allow for real-time magnetic resonance imaging of convection-enhanced delivery in the primate brain. Exp Neurol 196(2):381–389CrossRef Saito R et al (2005) Gadolinium-loaded liposomes allow for real-time magnetic resonance imaging of convection-enhanced delivery in the primate brain. Exp Neurol 196(2):381–389CrossRef
Zurück zum Zitat Schmid MH, Korting HC (1994) Liposomes - a drug carrier system for topical treatment in dermatology. Crit Rev Ther Drug Carrier Syst 11(2–3):97–118 Schmid MH, Korting HC (1994) Liposomes - a drug carrier system for topical treatment in dermatology. Crit Rev Ther Drug Carrier Syst 11(2–3):97–118
Zurück zum Zitat Shestopalov I, Tice JD, Ismagilov RF (2004) Multi-step synthesis of nanoparticles performed on millisecond time scale in a microfluidic droplet-based system. Lab Chip 4(4):316–321CrossRef Shestopalov I, Tice JD, Ismagilov RF (2004) Multi-step synthesis of nanoparticles performed on millisecond time scale in a microfluidic droplet-based system. Lab Chip 4(4):316–321CrossRef
Zurück zum Zitat Sounart TL et al (2007) Spatially-resolved analysis of nanoparticle nucleation and growth in a microfluidic reactor. Lab Chip 7(7):908–915CrossRef Sounart TL et al (2007) Spatially-resolved analysis of nanoparticle nucleation and growth in a microfluidic reactor. Lab Chip 7(7):908–915CrossRef
Zurück zum Zitat Stroock AD et al (2002) Chaotic mixer for microchannels. Science 295(5555):647–651CrossRef Stroock AD et al (2002) Chaotic mixer for microchannels. Science 295(5555):647–651CrossRef
Zurück zum Zitat Sugiura S et al (2001a) Interfacial tension driven monodispersed droplet formation from microfabricated channel array. Langmuir 17(18):5562–5566CrossRef Sugiura S et al (2001a) Interfacial tension driven monodispersed droplet formation from microfabricated channel array. Langmuir 17(18):5562–5566CrossRef
Zurück zum Zitat Sugiura S et al (2001b) Preparation characteristics of monodispersed water-in-oil emulsions using microchannel emulsification. J Chem Eng Japan 34(6):757–765CrossRef Sugiura S et al (2001b) Preparation characteristics of monodispersed water-in-oil emulsions using microchannel emulsification. J Chem Eng Japan 34(6):757–765CrossRef
Zurück zum Zitat Szoka F, Papahadjopoulos D (1980) Comparative properties and methods of preparation of lipid vesicles (liposomes). Annu Rev Biophys Bioeng 9:467–508CrossRef Szoka F, Papahadjopoulos D (1980) Comparative properties and methods of preparation of lipid vesicles (liposomes). Annu Rev Biophys Bioeng 9:467–508CrossRef
Zurück zum Zitat Templeton NS et al (1997) Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol 15(7):647–652CrossRef Templeton NS et al (1997) Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol 15(7):647–652CrossRef
Zurück zum Zitat Thorsen T et al (2001) Dynamic pattern formation in a vesicle-generating microfluidic device. Phys Rev Lett 86(18):4163–4166CrossRef Thorsen T et al (2001) Dynamic pattern formation in a vesicle-generating microfluidic device. Phys Rev Lett 86(18):4163–4166CrossRef
Zurück zum Zitat Trindade T, O’Brien P, Pickett NL (2001) Nanocrystalline semiconductors: Synthesis, properties, and perspectives. Chem Mater 13(11):3843–3858CrossRef Trindade T, O’Brien P, Pickett NL (2001) Nanocrystalline semiconductors: Synthesis, properties, and perspectives. Chem Mater 13(11):3843–3858CrossRef
Zurück zum Zitat Wagner A et al (2002) The crossflow injection technique: an improvement of the ethanol injection method. J Liposome Res 12(3):259–270CrossRef Wagner A et al (2002) The crossflow injection technique: an improvement of the ethanol injection method. J Liposome Res 12(3):259–270CrossRef
Zurück zum Zitat Wang HZ et al (2004) Continuous synthesis of CdSe-ZnS composite nanoparticles in a microfluidic reactor. Chem Commun (1):48–49 Wang HZ et al (2004) Continuous synthesis of CdSe-ZnS composite nanoparticles in a microfluidic reactor. Chem Commun (1):48–49
Zurück zum Zitat Wang HZ et al (2002) Preparation of titania particles utilizing the insoluble phase interface in a microchannel reactor. Chem Commun (14):1462–1463 Wang HZ et al (2002) Preparation of titania particles utilizing the insoluble phase interface in a microchannel reactor. Chem Commun (14):1462–1463
Zurück zum Zitat Wu L et al (2006) Droplet formation in microchannels under static conditions. Appl Phys Lett 89(14):Art. No. 144106 Wu L et al (2006) Droplet formation in microchannels under static conditions. Appl Phys Lett 89(14):Art. No. 144106
Metadaten
Titel
Preparation of nanoparticles by continuous-flow microfluidics
verfasst von
Andreas Jahn
Joseph E. Reiner
Wyatt N. Vreeland
Don L. DeVoe
Laurie E. Locascio
Michael Gaitan
Publikationsdatum
01.08.2008
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 6/2008
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-007-9340-5

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